园艺学报 ›› 2023, Vol. 50 ›› Issue (3): 495-507.doi: 10.16420/j.issn.0513-353x.2021-1282
刘语诺*, 曹亚*, 王帅, 杜美霞, 郑林, 陈善春, 邹修平**()
收稿日期:
2022-08-23
修回日期:
2022-12-21
出版日期:
2023-03-25
发布日期:
2023-04-03
通讯作者:
**(E-mail:zouxiuping@cric.cn)
作者简介:
*共同第一作者
基金资助:
LIU Yunuo*, CAO Ya*, WANG Shuai, DU Meixia, ZHENG Lin, CHEN Shanchun, ZOU Xiuping**()
Received:
2022-08-23
Revised:
2022-12-21
Online:
2023-03-25
Published:
2023-04-03
Contact:
**(E-mail:zouxiuping@cric.cn)
摘要:
MYB转录因子不仅在植物生长发育中起着重要作用,且在植物抗病反应中承担着重要的调节功能。本研究中以溃疡病感病品种纽荷尔脐橙(Citrus sinensis Osbeck)和抗病品种四季橘(C. madurensis)为材料,基于前期构建的溃疡病诱导柑橘转录组数据库,筛选获得2个受柑橘溃疡病菌Xanthomonas citri subsp. citri(Xcc)显著诱导的MYB基因:CsMYB41(Cs1g06220)和CsMYB63(Cs4g12760)。利用PCR技术克隆了纽荷尔脐橙的CsMYB41和CsMYB63,并对其结构特征及表达特性进行分析。PCR克隆测序分析发现,这2个基因的全长分别为1 304 bp、1 398 bp,开放阅读框(ORF)为1 047 bp、1 170 bp,各编码349、390个氨基酸。蛋白质同源序列比对和结构分析表明,这2个基因属于MYB类转录因子家族中的R2R3-MYB亚家族;进化树分析表明,CsMYB41和CsMYB63蛋白分别与可可、蓖麻等MYB41和MYB63蛋白高度同源。亚细胞定位结果显示,CsMYB41和CsMYB63定位在细胞核中。实时荧光定量PCR(qRT-PCR)分析表明,CsMYB41和CsMYB63均受Xcc诱导显著上调表达。激素诱导试验表明,在纽荷尔脐橙中,乙烯利(ETH)、水杨酸(SA)、脱落酸(ABA)诱导CsMYB41上调表达,而ETH、SA、茉莉酸(JA)、ABA诱导CsMYB63上调表达。在四季橘中,CsMYB41受ETH、SA诱导上调表达,CsMYB63仅受IAA诱导上调表达。以上结果表明,这2个MYB基因可能在应答柑橘溃疡病菌侵染中起重要作用。
中图分类号:
刘语诺, 曹亚, 王帅, 杜美霞, 郑林, 陈善春, 邹修平. 柑橘CsMYB41和CsMYB63响应溃疡病菌侵染的表达[J]. 园艺学报, 2023, 50(3): 495-507.
LIU Yunuo, CAO Ya, WANG Shuai, DU Meixia, ZHENG Lin, CHEN Shanchun, ZOU Xiuping. Expression Analysis of CsMYB41 and CsMYB63 Genes in Response to Citrus Canker[J]. Acta Horticulturae Sinica, 2023, 50(3): 495-507.
引物名称 Primer | 序列(5′-3′) Sequences | 引物名称 Primer | 序列(5′-3′) Sequences |
---|---|---|---|
qCs1g06220-F | AAGCTTGTCGAGTAATTGAAATGGG | qCs1g06220-R | TTATTATACAAGTCCAATCGCATCC |
qCs4g12760-F | ATTATCTCAAATGGGAAAAGGGAGA | qCs4g12760-R | AATTATTTGATCACTCGCAAACGGA |
Cs1g06220-F | CGGGGTACC ATGGGAAGGCCACCAAGCTC | Cs1g06220-R | CGCGGATCC TACAAGTCCAATCGCATCCA |
Cs4g12760-F | CGGGGTACC ATGGGAAAAGGGAGAGCTCC | Cs4g12760-R | CGCGGATCC CTCGCAAACGGAACTTTGTA |
Actin-F | CATCCCTCAGCACCTTCC | Actin-R | CCAACCTTAGCACTTCTCC |
表1 本研究中所用的引物
Table 1 Primers used in this study
引物名称 Primer | 序列(5′-3′) Sequences | 引物名称 Primer | 序列(5′-3′) Sequences |
---|---|---|---|
qCs1g06220-F | AAGCTTGTCGAGTAATTGAAATGGG | qCs1g06220-R | TTATTATACAAGTCCAATCGCATCC |
qCs4g12760-F | ATTATCTCAAATGGGAAAAGGGAGA | qCs4g12760-R | AATTATTTGATCACTCGCAAACGGA |
Cs1g06220-F | CGGGGTACC ATGGGAAGGCCACCAAGCTC | Cs1g06220-R | CGCGGATCC TACAAGTCCAATCGCATCCA |
Cs4g12760-F | CGGGGTACC ATGGGAAAAGGGAGAGCTCC | Cs4g12760-R | CGCGGATCC CTCGCAAACGGAACTTTGTA |
Actin-F | CATCCCTCAGCACCTTCC | Actin-R | CCAACCTTAGCACTTCTCC |
基因ID Gene ID | 基因描述 Gene description | 表达差异倍数 log2 fold change | |||||
---|---|---|---|---|---|---|---|
纽荷尔脐橙 Newhall Navel Orange | 四季橘 Calamondin | ||||||
1 d | 3 d | 5 d | 1 d | 3 d | 5 d | ||
Cs1g01670 | MYB93一种新的侧根发育负调控因子 MYB93 a novel negative regulator of lateral root development | 0.0 | 0.0 | 0.0 | 0.0 | 3.6 | 4.7 |
Cs1g06220 | MYB41参与创伤和渗透应激反应 MYB41 involved in wounding and osmotic stress response | 0.0 | 4.5 | 4.7 | 0.0 | 0.0 | 0.0 |
Cs1g19400 | MYB7参与原花青素的合成 MYB7 involved in proanthocyanidins synthesis | 2.5 | 4.0 | 6.1 | 1.1 | 0.4 | 3.3 |
Cs1g19970 | MYB78响应脱落酸和盐胁迫 MYB78 responses to abscisic acid and salt stress | 4.3 | 4.4 | 5.5 | 3.0 | 3.9 | 4.7 |
Cs2g06950 | MYB样蛋白102参与创伤和渗透应激 MYB-like 102 involved in wounding and osmotic stress | 3.4 | 5.1 | 7.1 | 3.1 | 2.3 | 2.8 |
Cs3g02020 | MYB同源结构域样超家族蛋白 MYB Homeodomain-like superfamily protein | 2.2 | 3.4 | 5.2 | 1.0 | 1.0 | 1.8 |
Cs3g22220 | MYB6参与调节花青素生物合成 MYB6 involved in regulating anthocyanin biosynthesis | 3.2 | 4.0 | 5.0 | 2.5 | 3.0 | 4.3 |
Cs3g23070 | MYBR1参与多种非生物刺激反应 MYBR1 involved in a variety of abiotic stimiuli response | 1.9 | 3.3 | 3.8 | 1.3 | 1.9 | 2.5 |
Cs4g07780 | MYB103是花药发育所必需的 MYB103 is required for anther development | 4.0 | 3.4 | 5.4 | 2.6 | 3.7 | 4.9 |
Cs4g12760 | MYB63参与细胞壁发育 MYB63 involved in cell wall development | 8.5 | 10.3 | 11.2 | 6.2 | 5.9 | 7.5 |
Cs4g18910 | MYB36参与侧根边界的发育 MYB36 involved in develompent of lateral root boundaries | 1.0 | -0.5 | -0.8 | 1.2 | 2.0 | 2.4 |
Cs5g10670 | MYB96在干旱胁迫期间介导脱落酸信号传导 MYB96 mediates abscisic acid signaling during drought stress | -1.5 | -1.4 | -8.1 | -0.9 | -1.2 | -3.4 |
Cs5g26420 | 同源结构域样超家族蛋白 Homeodomain-like superfamily protein | -1.1 | -1.9 | -3.8 | -0.5 | -0.8 | -2.1 |
Cs5g27440 | MYB117在器官模式中发挥功能 MYB117 functions in organ patterning | 2.0 | 1.1 | -0.8 | 1.7 | 1.1 | 0.7 |
Cs5g29830 | MYB14 | 2.1 | 0.6 | 1.1 | 3.6 | 2.1 | 1.5 |
Cs6g17340 | MYB20 | 4.2 | 2.4 | 0.6 | 3.8 | 2.8 | 1.4 |
Cs7g26930 | MYB62参与赤霉素生物合成的调节 MYB62 involved in regulation of gibberellic acid biosynthesis | 3.0 | 3.7 | 4.0 | 1.9 | 1.6 | 2.0 |
Cs7g32400 | TBP1具有单个MYB端粒DNA结合域 TBP1 with a single MYB telomeric DNA-binding domain | 0.2 | 0.8 | 3.2 | 0.0 | 0.0 | 1.0 |
Cs8g12680 | MYB4相关蛋白 MYB-related protein MYB4 | 1.5 | 2.1 | 2.6 | 2.5 | 2.5 | 2.2 |
Cs9g01750 | 推测转录因子MYB108 putative transcription factor MYB108 | 2.1 | 5.0 | 6.1 | 1.9 | 2.5 | 4.7 |
Orange1.1t00897 | MYB38调节腋生分生组织的形成 MYB38 regulates axillary meristem formation | 2.9 | 4.2 | 5.6 | 2.4 | 3.9 | 3.7 |
表2 柑橘响应溃疡病菌侵染的MYB家族差异表达基因
Table 2 Differentially expressed MYB genes responding to Xcc infection
基因ID Gene ID | 基因描述 Gene description | 表达差异倍数 log2 fold change | |||||
---|---|---|---|---|---|---|---|
纽荷尔脐橙 Newhall Navel Orange | 四季橘 Calamondin | ||||||
1 d | 3 d | 5 d | 1 d | 3 d | 5 d | ||
Cs1g01670 | MYB93一种新的侧根发育负调控因子 MYB93 a novel negative regulator of lateral root development | 0.0 | 0.0 | 0.0 | 0.0 | 3.6 | 4.7 |
Cs1g06220 | MYB41参与创伤和渗透应激反应 MYB41 involved in wounding and osmotic stress response | 0.0 | 4.5 | 4.7 | 0.0 | 0.0 | 0.0 |
Cs1g19400 | MYB7参与原花青素的合成 MYB7 involved in proanthocyanidins synthesis | 2.5 | 4.0 | 6.1 | 1.1 | 0.4 | 3.3 |
Cs1g19970 | MYB78响应脱落酸和盐胁迫 MYB78 responses to abscisic acid and salt stress | 4.3 | 4.4 | 5.5 | 3.0 | 3.9 | 4.7 |
Cs2g06950 | MYB样蛋白102参与创伤和渗透应激 MYB-like 102 involved in wounding and osmotic stress | 3.4 | 5.1 | 7.1 | 3.1 | 2.3 | 2.8 |
Cs3g02020 | MYB同源结构域样超家族蛋白 MYB Homeodomain-like superfamily protein | 2.2 | 3.4 | 5.2 | 1.0 | 1.0 | 1.8 |
Cs3g22220 | MYB6参与调节花青素生物合成 MYB6 involved in regulating anthocyanin biosynthesis | 3.2 | 4.0 | 5.0 | 2.5 | 3.0 | 4.3 |
Cs3g23070 | MYBR1参与多种非生物刺激反应 MYBR1 involved in a variety of abiotic stimiuli response | 1.9 | 3.3 | 3.8 | 1.3 | 1.9 | 2.5 |
Cs4g07780 | MYB103是花药发育所必需的 MYB103 is required for anther development | 4.0 | 3.4 | 5.4 | 2.6 | 3.7 | 4.9 |
Cs4g12760 | MYB63参与细胞壁发育 MYB63 involved in cell wall development | 8.5 | 10.3 | 11.2 | 6.2 | 5.9 | 7.5 |
Cs4g18910 | MYB36参与侧根边界的发育 MYB36 involved in develompent of lateral root boundaries | 1.0 | -0.5 | -0.8 | 1.2 | 2.0 | 2.4 |
Cs5g10670 | MYB96在干旱胁迫期间介导脱落酸信号传导 MYB96 mediates abscisic acid signaling during drought stress | -1.5 | -1.4 | -8.1 | -0.9 | -1.2 | -3.4 |
Cs5g26420 | 同源结构域样超家族蛋白 Homeodomain-like superfamily protein | -1.1 | -1.9 | -3.8 | -0.5 | -0.8 | -2.1 |
Cs5g27440 | MYB117在器官模式中发挥功能 MYB117 functions in organ patterning | 2.0 | 1.1 | -0.8 | 1.7 | 1.1 | 0.7 |
Cs5g29830 | MYB14 | 2.1 | 0.6 | 1.1 | 3.6 | 2.1 | 1.5 |
Cs6g17340 | MYB20 | 4.2 | 2.4 | 0.6 | 3.8 | 2.8 | 1.4 |
Cs7g26930 | MYB62参与赤霉素生物合成的调节 MYB62 involved in regulation of gibberellic acid biosynthesis | 3.0 | 3.7 | 4.0 | 1.9 | 1.6 | 2.0 |
Cs7g32400 | TBP1具有单个MYB端粒DNA结合域 TBP1 with a single MYB telomeric DNA-binding domain | 0.2 | 0.8 | 3.2 | 0.0 | 0.0 | 1.0 |
Cs8g12680 | MYB4相关蛋白 MYB-related protein MYB4 | 1.5 | 2.1 | 2.6 | 2.5 | 2.5 | 2.2 |
Cs9g01750 | 推测转录因子MYB108 putative transcription factor MYB108 | 2.1 | 5.0 | 6.1 | 1.9 | 2.5 | 4.7 |
Orange1.1t00897 | MYB38调节腋生分生组织的形成 MYB38 regulates axillary meristem formation | 2.9 | 4.2 | 5.6 | 2.4 | 3.9 | 3.7 |
图2 柑橘CsMYB41和CsMYB63与其他植物MYB蛋白的多序列比对 下划线示核定位信号序列。
Fig. 2 Alignment of the CsMYB41 and CsMYB63 amino acid sequences with MYB transcription factors from other species Underlines indicate nuclear localization signal sequence.
图3 不同植物MYB基因编码氨基酸序列系统发育树分析 标尺表示不同植物序列间差异数值的单位长度;数字表示进化分支长度。
Fig. 3 Phylogenetic analysis of deduced amino acid sequences of MYB from different plants The ruler represents the unit length of the difference value between different plant sequences;The number represents the length of the evolutionary branch.
图5 CsMYB41和CsMYB63在柑橘叶片接种溃疡病原菌后的表达特征 图柱上不同字母表示与0 d处理比较的差异显著性(Tukey’s检测,P < 0.05)。
Fig. 5 The expression characteristics of CsMYB41 and CsMYB63 genes in citrus leaves infected by Xanthomonas citri subsp. citri(Xcc) Different letters on the top of bar represented significant differences compared to the 0 d treatment(Tukey’s test,P < 0.05).
图6 柑橘CsMYB41受植物生长调节剂诱导表达 图柱上不同字母表示与0 h处理比较的差异显著性(Tukey’s检测,P < 0.05)。下同。
Fig. 6 CsMYB41 expression induced by exogenous plant growth regulators Different letters on the top of bar represented significant differences compared to the 0 h treatment(Tukey’s test,P < 0.05). The same below.
[1] |
Chen Y, Yang X, He K, Liu M, Li J, Gao Z, Lin Z, Zhang Y, Wang X, Qiu X, Shen Y, Zhang L, Deng X, Luo J, Deng X, Chen Z, Gu H, Qu L. 2006. The MYB transcription factor superfamily of Arabidopsis:expression analysis and phylogenetic comparison with the rice MYB family. Plant Molecular Biology, 60:107-124.
doi: 10.1007/s11103-005-2910-y URL |
[2] | Dai Wen Shan, Wu Yue, Wang Min. 2022. Preliminary study on mechanisms of resistance to citrus canker of kumquat FcRGA1. Acta Horticulturae Sinica, 49 (11):2325-2335. (in Chinese) |
戴文珊, 吴玥, 王敏. 2022. 金柑FcRGA1抗溃疡病机制初探. 园艺学报, 49 (11):2325-2335.
doi: 10.16420/j.issn.0513-353x.2021-0940 URL |
|
[3] |
Elizabeth S H, Thomas E C, Hank W B. 2012. Maize histone H2B-mCherry:a new fluorescent chromatin marker for somatic and meiotic chromosome research. DNA and Cell Biology, 31:925-938.
doi: 10.1089/dna.2011.1514 pmid: 22662764 |
[4] |
Han Jinheng, Wang Lixia, Gao Hongbo, Lü Guiyun. 2016. Cloning and expression analysis of Fusarium wilt resistance related gene CIMYB transcription factor from Citrullus lanatus. Scientia Agricultura Sinica, 49 (17):3359-3369. (in Chinese)
doi: 10.3864/j.issn.0578-1752.2016.17.010 |
韩金桓, 王丽霞, 高洪波, 吕桂云. 2016. 西瓜抗枯萎病相关基因CIMYB转录因子的克隆及表达分析. 中国农业科学, 49 (17):3359-3369.
doi: 10.3864/j.issn.0578-1752.2016.17.010 |
|
[5] | He Xiuling, Yuan Hongxu. 2007. Research advancesonthe occurrence and resistance of citrus bacterial canker disease. Chinese Agricultural Science Bulletin,(8):409-412. (in Chinese) |
何秀玲, 袁红旭. 2007. 柑橘溃疡病发生与抗性研究进展. 中国农学通报,(8):409-412. | |
[6] |
Ji Miaomiao, Wan Ye, Zhang Yiping, Ma Hai, Wang Xiping, Gao Hua. 2021. Studies on the resistance of apple MdMYB116 to powdery mildew. Acta Horticulturae Sinica, 48 (11):2133-2145. (in Chinese)
doi: 10.16420/j.issn.0513-353x.2021-0060 URL |
吉苗苗, 万叶, 张一平, 马海, 王西平, 高华. 2021. 苹果MdMYB116对白粉病的抗性研究. 园艺学报, 48 (11):2133-2145.
doi: 10.16420/j.issn.0513-353x.2021-0060 URL |
|
[7] | Ju Hongping. 2012. Functional characterization of herbivore resistance-related gene OsJAMYB in rice[M. D. Dissertation]. Hangzhou: Zhejiang University. (in Chinese) |
鞠红平. 2012. 水稻抗虫相关基因OsJAMYB功能解析[硕士论文]. 杭州: 浙江大学. | |
[8] |
Ke Yujie, Chen Mingkun, Ma Shanhu, Ou Yue, Wang Yi, Zheng Qingdong, Liu Zhongjian, Ai Ye. 2021. Research progress of MYB transcription factors in Orchidaceae. Acta Horticulturae Sinica, 48 (11):2311-2320. (in Chinese)
doi: 10.16420/j.issn.0513-353x.2020-0843 URL |
柯玉洁, 陈明堃, 马山虎, 欧悦, 王艺, 郑清冬, 刘仲健, 艾叶. 2021. 兰科植物MYB转录因子研究进展. 园艺学报, 48 (11):2311-2320.
doi: 10.16420/j.issn.0513-353x.2020-0843 URL |
|
[9] |
Kosma D K, Murmu J, Razeq F M, Santos P, Bourgault R, Molina I, Rowland O. 2014. AtMYB 41 activates ectopic suberin synthesis and assembly in multiple plant species and cell types. Plant Journal, 80 (2):216-229.
doi: 10.1111/tpj.2014.80.issue-2 URL |
[10] |
Li W, Zhu Z, Chern M, Yin J, Yang C, Ran L, Cheng M, He M, Wang K, Wang J, Zhou X, Zhu X, Chen Z, Wang J, Zhao W, Ma B, Qin P, Chen W, Wang Y, Liu J, Wang W, Wu X, Li P, Wang J, Zhu L, Li S, Chen X. 2017. A natural allele of a transcription factor in rice confers broad-spectrum blast resistance. Cell, 170 (1):114-126.
doi: S0092-8674(17)30649-9 pmid: 28666113 |
[11] |
Lipsick J S. 1996. One billion years of Myb. Oncogene, 13 (2):223-235.
pmid: 8710361 |
[12] |
Long Q, Xie Y, He Y, Li Q, Zou X P, Chen S C. 2019. Abscisic acid promotes jasmonic acid accumulation and plays a key role in citrus canker development. Frontiers in Plant Science, 10:1634.
doi: 10.3389/fpls.2019.01634 pmid: 31921273 |
[13] | Mabuchi K, Maki H, Itaya T, Suzuki T, Nomoto M, Sakaoka S, Morikami A, Higashiyama T, Tada Y, Busch W, Tsukagoshi H. 2018. MYB30 links ROS signaling,root cell elongation,and plant immune responses. Proceedings of the National Academy of Sciences of the United States of America, 115 (20):E4710. |
[14] | Niu Yiling, Jiang Xiuming, Xu Xiangyang. 2016. Reaserch advances on transcription factor MYB gene family in plant. Molecular Plant Breeding, 14 (8):2050-2059. (in Chinese) |
牛义岭, 姜秀明, 许向阳. 2016. 植物转录因子MYB基因家族的研究进展. 分子植物育种, 14 (8):2050-2059. | |
[15] |
Pitino M, Armstrong C M, Duan Y. 2015. Rapid screening for citrus canker resistance employing pathogen-associated molecular pattern-triggered immunity responses. Horticulture Research, 2:15042.
doi: 10.1038/hortres.2015.42 pmid: 26504581 |
[16] | Qiao Meng, Xiang Fengning. 2009. The Arabidopsis AtMYB96 transcription factor in the regulation of ABA-dependent drought stress response// Cell,Life and Health—Proceedings of the 11th Chinese Cell Biology Conference and 2009 Xi’an International Conference on Cell Biology. Xi’an:Chinese Society for Cell Biology:187. (in Chinese) |
乔孟, 向凤宁. 2009. 拟南芥atMYB96转录因子参与调控依赖ABA的干旱胁迫响应//细胞 · 生命 · 健康——第十一届中国细胞生物学学术大会暨2009西安细胞生物学国际会议论文集. 西安:中国细胞生物学学会:187. | |
[17] | Qiao Meng, Yu Yanchong, Xiang Fengning. 2009. The roles of the Arabidopsis R2R3-MYB transcription factors in the stress responses. Chinese Bulletin of Life Sciences, 21 (1):145-150. (in Chinese) |
乔孟, 于延冲, 向凤宁. 2009. 拟南芥R2R3-MYB类转录因子在环境胁迫中的作用. 生命科学, 21 (1):145-150. | |
[18] |
Raffaele S, Vailleau F, Léger A, Joubès J, Miersch O, Huard C, Blée E, Mongrand S, Domergue F, Roby D. 2008. A MYB transcription factor regulates very-long-chain fatty acid biosynthesis for activation of the hypersensitive cell death response in Arabidopsis. Plant Cell, 20 (3):752-767.
doi: 10.1105/tpc.107.054858 pmid: 18326828 |
[19] |
Shen L, Li B, Ren Y, Li C, Zhong Y, Fang C, Lin W. 2015. Phenolic acid synthesis of allelopathic rice regulated by OsMYB and its weed inhibition. Acta Agronomica Sinica, 41 (4):531.
doi: 10.3724/SP.J.1006.2015.00531 URL |
[20] |
Stracke R, Werber M, Weisshaar B. 2001. The R2R3-MYB gene family in Arabidopsis thaliana. Current Opinion in Plant Biology, 4 (5):447-456.
doi: 10.1016/s1369-5266(00)00199-0 pmid: 11597504 |
[21] |
Van D E S, Verhagen B W, Van D R, Bakker D, Verlaan M G, Pel M J, Joosten R G, Proveniers M C, Van Loon L C, Ton J, Pieterse C M. 2008. MYB 72 is required in early signaling steps of rhizobacteria-induced systemic resistance in Arabidopsis. Plant Physiology, 146 (3):1293.
doi: 10.1104/pp.107.113829 URL |
[22] | Wang Xinyue. 2003. Preliminary studies on Agrobaacterium tumefaciens-mediated transformation of bush bean by floral spraying[M. D. Dissertation]. Beijing: China Agricultural University. (in Chinese) |
王欣月. 2003. 花器喷雾法接种根癌农杆菌转化矮生菜豆条件的初步探索[硕士论文]. 北京: 中国农业大学. | |
[23] | Wang Zhongkang, Sun Xianyun, Xia Yuxian, Zhou Changyong, Yin Youping. 2004. Rapid detection of citrus bacterial canker disease(Xanthomonas axonopodis pv. citri)with polymerase chain reaction(PCR). Acta Phytopathologica Sinica, 34 (1):14-20. (in Chinese) |
王中康, 孙宪昀, 夏玉先, 周常勇, 殷幼平. 2004. 柑桔溃疡病菌PCR快速检验检疫技术研究. 植物病理学报, 34 (1):14-20. | |
[24] | Wei X, Mao L, Wei X, Xia M, Xu C. 2020. MYB41,MYB107,and MYC 2 promote ABA-mediated primary fatty alcohol accumulation via activation of AchnFAR in wound suberization in kiwifruit. Horticulture Researsh,(7):86. |
[25] | Yao Lixiao, He Yongrui, Zou Xiuping, Lei Tiangang, Xu Lanzhen, Peng Aihong, Chen Shanchun. 2013. Advances and strategies in citrus genetic engineering and breeding. Journal of Fruit Science, 30 (6):1056-1064. (in Chinese) |
姚利晓, 何永睿, 邹修平, 雷天刚, 许兰珍, 彭爱红, 陈善春. 2013. 柑橘基因工程育种研究策略及其进展. 果树学报, 30 (6):1056-1064. | |
[26] | Yao Qintao, Zhang Wenwei, Liu Li, Fu Yueling, Han Rong, Duan Jiangyan, Qi Fangjun. 2008. Functional analysis of LIM transcription factor(OsLIM)involved in rice non-host disease resistance//Innovation and Development of Plant Protection Technology. Beijing: China Agricultural Science and Technology Press:483-490. (in Chinese) |
姚沁涛, 张文蔚, 刘莉, 付月灵, 韩榕, 段江燕, 齐放军. 2008. LIM转录因子(OsLIM)参与水稻非寄主抗病性的功能分析//植物保护科技创新与发展. 北京: 中国农业科学技术出版社:483-490. | |
[27] | Yuan Y, Qi L, Yang J, Wu C, Liu Y, Huang L. 2015. Erratum to:a Scutellaria baicalensis R2R3-MYB gene,SbMYB8,regulates flavonoid biosynthesis and improves drought stress tolerance in transgenic tobacco. Plant Cell,Tissue and Organ Culture(PCTOC), 120 (3):973. |
[28] |
Zamioudis C, Korteland J, Van Pelt J A, Hamersveld M, Dombrowski N, Bai Y, Hanson J, Verk V M C, Ling H, Schulze L P, Pirterse C M J. 2015. Rhizobacterial volatiles and photosynthesis-related signals coordinate MYB 72 expression in Arabidopsis roots during onset of induced systemic resistance and iron-deficiency responses. Plant Journal for Cell and Molecular Biology, 84 (2):309-322.
doi: 10.1111/tpj.2015.84.issue-2 URL |
[29] |
Zhang X D, Francis M I, Dawson W O, Graham J H, Orbović V, Triplett E W, Mou Z. 2010. Over-expression of the Arabidopsis NPR1 gene in citrus increases resistance to citrus canker. European Journal of Plant Pathology, 128 (1):91-100.
doi: 10.1007/s10658-010-9633-x URL |
[30] | Zhen Lin, Wang Shuai, Liu Yunuo, Du Meixia, Peng Aihong, He Yongrui, Chen Shanchun, Zou Xiuping. 2022. Gene cloning and expression analysis of NAC gene in citrus in response to Huanglongbing. Acta Horticulturae Sinica, 49 (7):1441-1457. (in Chinese) |
郑林, 王帅, 刘语诺, 杜美霞, 彭爱红, 何永睿, 陈善春, 邹修平. 2022. 柑橘响应黄龙病菌侵染的NAC基因的克隆及表达分析. 园艺学报, 49 (7):1441-1457.
doi: 10.16420/j.issn.0513-353x.2021-0553 |
|
[31] |
Zhou J, Lee C, Zhong R, Ye Z H. 2009. MYB58 and MYB 63 are transcriptional activators of the lignin biosynthetic pathway during secondary cell wall formation in Arabidopsis. Plant Cell, 21 (1):248-266.
doi: 10.1105/tpc.108.063321 URL |
[32] | Zou Xiuping, Peng Aihong, Liu Qiqi, He Yongrui, Wang Junzheng, Xu Lanzhen, Lei Tiangang, Yao Lixiao, Chen Shanchun. 2014. Secreted expression of cecropin B gene enhances resistance to Xanthomonas axonopodis pv. citri in transgenic Citrus sinensis‘Tarocco’. Acta Horticulturae Sinica, 41 (3):417-428. (in Chinese) |
邹修平, 彭爱红, 刘琦琦, 何永睿, 王军政, 许兰珍, 雷天刚, 姚利晓, 陈善春. 2014. 分泌型Cecropin B抗菌肽基因转化血橙提高其抗溃疡病水平. 园艺学报, 41 (3):417-428. |
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