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园艺学报 ›› 2022, Vol. 49 ›› Issue (5): 1023-1030.doi: 10.16420/j.issn.0513-353x.2021-0732

• 研究论文 • 上一篇    下一篇

甜樱桃PavMYB10.1促进PavRiant表达和花青苷积累

李丽仙1, 王烁1, 陈莹1, 邬滢涛1, 王雅倩1, 房月1, 陈学森1, 田长平2, 冯守千1,*()   

  1. 1山东农业大学园艺科学与工程学院,山东农业大学作物生物学国家重点实验室,山东泰安 271018
    2烟台市农业科学研究院,山东烟台 265599
  • 收稿日期:2022-01-04 修回日期:2022-03-28 出版日期:2022-05-25 发布日期:2022-05-25
  • 通讯作者: 冯守千 E-mail:shouqianlove@sdau.edu.cn
  • 基金资助:
    国家重点研发计划项目(2018YFD1000105);山东省良种工程项目(2019LZGC008);国家自然科学基金项目(31201593);国家自然科学基金项目(31872940)

PavMYB10.1 Promotes Anthocyanin Accumulation by Positively Regulating PavRiant in Sweet Cherry

LI Lixian1, WANG Shuo1, CHEN Ying1, WU Yingtao1, WANG Yaqian1, FANG Yue1, CHEN Xuesen1, TIAN Changping2, FENG Shouqian1,*()   

  1. 1State Key Laboratory of Crop Biology,College of Horticulture Science and Engineering,Shandong Agricultural University,Tai’an,Shandong 271018,China
    2Yantai Academy of Agricultural Sciences,Yantai,Shandong 265599,China
  • Received:2022-01-04 Revised:2022-03-28 Online:2022-05-25 Published:2022-05-25
  • Contact: FENG Shouqian E-mail:shouqianlove@sdau.edu.cn

摘要:

为了解谷胱甘肽S-转移酶(GST)参与甜樱桃花青苷积累的机制,以甜樱桃‘美早’为试材,克隆1个phi类型GST基因PavRiant(XM_021968160)。通过系统进化树分析、qRT-PCR、瞬时表达沉默、酵母单杂交、电泳迁移率试验和荧光素酶报告试验,探究PavRiant调控甜樱桃花青苷积累的作用。系统进化树分析表明,PavRiant与桃PpRiant蛋白序列相似度最高。果实发育期,PavRiant表达量与花青苷含量和花青苷调控基因PavMYB10.1表达量变化趋势一致。瞬时沉默试验证实PavRiant在甜樱桃花青苷积累调控中发挥重要作用。酵母单杂交和EMSA试验发现,PavMYB10.1结合PavRiant启动子的MRE序列。荧光素酶试验表明PavRiant启动子活性受PavMYB10.1正调控。因此,PavMYB10.1可能通过促进PavRiant的表达促进花青苷的积累。

关键词: 甜樱桃, 花青苷, PavMYB10.1, PavRiant, 谷胱甘肽S-转移酶

Abstract:

To understand how GST regulates anthocyanin accumulation in sweet cherry,a phi-type GST gene PavRiant(XM_021968160)was cloned from the sweet cherry cultivar‘Tieton’. The effects of PavRiant on anthocyanin accumulation in sweet cherry were investigated using phylogenetic tree analysis,real-time reverse transcription-polymerase chain reaction,transient silencing assay,yeast one-hybrid(Y1H),electrophoretic mobility shift assay(EMSA),and luciferase reporter assay. Phylogenetic tree analysis showed that PavRiant has the highest similarity to the PpRiant protein sequence of peach. During sweet cherry fruit development,the changing trends of PavRiant expressions were consistent with anthocyanin contents and PavMYB10.1 expressions. Further,the transient silencing assay showed that PavRiant plays an essential role in regulating anthocyanin accumulation. Y1H and EMSA experiments showed that PavMYB10.1 binds to the MRE sequence of the PavRiant promoter. Finally,the luciferase assay showed that PavMYB10.1 positively regulates the activity of the PavRiant promoter. Therefore,PavMYB10.1 can promote anthocyanin accumulation by positively regulating PavRiant expression in sweet cherry.

Key words: sweet cherry, anthocyanin, PavMYB10.1, PavRiant, GST

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