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园艺学报 ›› 2021, Vol. 48 ›› Issue (5): 987-1001.doi: 10.16420/j.issn.0513-353x.2020-0600

• 研究论文 • 上一篇    下一篇

辣椒B-box转录因子家族的鉴定及表达分析

胡景涛*(), 阮宇, 甘丽萍   

  1. 重庆三峡学院生物与食品工程学院,重庆万州 404100
  • 收稿日期:2021-02-23 修回日期:2021-03-19 出版日期:2021-05-25 发布日期:2021-06-07
  • 作者简介:* E-mail: 20180016@sanxiau.edu.cn
  • 基金资助:
    重庆市自然科学基金面上项目(cstc2020jcyj-msxmX0073);重庆三峡学院博士引进项目(2014/09925401);重庆市渝东北特色生物资源开发利用工程技术研究中心项目(1209/061800406)

Identification and Expression Analysis of the B-box Transcription Factor Family in Pepper

HU Jingtao*(), RUAN Yu, GAN Liping   

  1. College of Biology and Food Engineering,Chongqing Three Gorges University,Wanzhou,Chongqing 404100,China
  • Received:2021-02-23 Revised:2021-03-19 Online:2021-05-25 Published:2021-06-07

摘要:

以辣椒基因组数据为基础,利用生物信息学方法,对辣椒B-box家族基因进行了染色体定位、基因结构、系统进化及组织表达模式等分析,并通过qRT-PCR方法对该家族基因在激素及非生物胁迫下的转录水平进行了检测。辣椒全基因组中共鉴定出26个BBX家族成员,分布于辣椒12条染色体上。系统进化分析分析发现,辣椒BBX蛋白成员可进一步分为5类:groupⅠ~Ⅴ。其中,groupⅠ包含两个B-box结构域;group Ⅱ和Ⅲ中的蛋白除CaBBX25外,都包含B-box1、B-box2和CCT结构域;group Ⅳ仅包含1个B-box结构域;groupⅤ含有1个B-box和1个CCT结构域。同一进化分类中的成员通常具有相同或者相似的外显子数,其蛋白中保守基序的构成也具有较高的相似性。组织特异性表达分析发现,除D类基因外,其他17个基因在不同器官或者果实的不同发育时期都有着较高的表达水平。qRT-PCR分析发现,10个CaBBX基因受到激素和非生物胁迫不同程度的诱导表达。

关键词: 辣椒, B-box转录因子, 鉴定, 表达分析

Abstract:

Based on the genome data of pepper,the chromosomal localization,gene structure,phylogenetic relationships and tissue-specific expression of B-box genes in pepper were analyzed by bioinformatics method. Moreover,the transcription level of the family genes under hormone and abiotic stress was detected by qRT-PCR. A total of 26 BBX members,which were distributed on 12 chromosomes,have been identified in pepper genome. Phylogenetic analysis showed that the CaBBX proteins could further be divided into five groups:groupⅠ-Ⅴ. Among them,members in groupⅠcontains two B-box domains. All the proteins in groupⅡandⅢcontained B-box1,B-box2 and CCT domains,except for CaBBX25. The proteins in group Ⅳ only had a B-box domain while members in group Ⅴ contained a B-box and a CCT domain. A same or similar number of exons has been found in members of the same clade usually. At the same time,a higher similar of the composition of motifs was also observed. Tissue specific expression analysis showed that the other 17 genes were highly expressed in different organs or at different devolpment stages of fruits,except for the class D genes. The result of qRT-PCR showed that 10 CaBBX genes could be promoted or repressed by the hormones and abiotic stresses.

Key words: pepper, B-box transcription factor, identification, expression analysis

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