关键词: 柑橘, 原生质体融合, 胞质杂种, 雄性不育

Abstract: ‘Huayou 2’is a male sterile somatic cybrid produced by protoplast fusion with‘Guoqing 1’Satsuma mandarin as callus parent and‘Huayou 1’（‘Hirado Buntan’pummelo）as leaf parent. It grows vigorously but with aberrant petals and stamens in flowers，resulting in pollen sterility relative to that of ‘Huayou 1’. The fruit of‘Huayou 2’is oblate in shape and seedless when cultivated separately from other cultivars. The average fruit weight is 1 232.47 g and the peel thickness is medium with the edible rate of 57.03%. The flesh is pink in color，goluptious，succulent and fibreless，with the total soluble solids（TSS）content，total acid（TA）content and vitamin C contents accounting for 12.37%–13.33%，0.90%–1.20% and 24.26 mL · L-1，respectively. In Wuhan，the fruit ripening period is during November and December. The yield per 5-year grafted tree can reach 50 kg.

Key words: citrus, protoplast fusion, somatic cybrid, male sterility