园艺学报 ›› 2018, Vol. 45 ›› Issue (3): 571-578.doi: 10.16420/j.issn.0513-353x.2017-0830

• 研究报告 • 上一篇    下一篇


申 琪1,欧承刚1,孙婷婷1,刘 波1,曹琼文1,赵志伟1,孔小平2,*,庄飞云1,2,*   

  1. (1中国农业科学院蔬菜花卉研究所,农业部园艺作物生物学与种质创制重点实验室,北京 100081;2西宁市蔬菜研究所,西宁 810000)
  • 出版日期:2018-03-25 发布日期:2018-03-25

QTL Analysis of Date of Initial Flowering,Main Stalk Length and Seed Weight per Plant in Carrot

SHEN Qi1,OU Chenggang1,SUN Tingting1,LIU Bo1,CAO Qiongwen1,ZHAO Zhiwei1,KONG Xiaoping2,*,and ZHUANG Feiyun1,2,*   

  1. Xiaoping2,*,and ZHUANG Feiyun1,2,* (1The Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Key Laboratory of Horticultural Crop Biology and Germplasm Innovation,Ministry of Agriculture,Beijing 100081,China;2Institue of Xining Vegetables,Xining 810000,China)
  • Online:2018-03-25 Published:2018-03-25

摘要: 以‘松滋野生’胡萝卜和橘色栽培品种‘Amsterdam’构建的回交重组自交系为试材,深入分析‘松滋野生’染色体片段对后代植株始花期、主薹高及单株种子量的遗传影响。试验结果表明,始花期在两年间均为偏正态分布,而主薹高均呈正态分布,单株种子量仅在2015年表现为正态分布。方差分析表明,3个性状在基因型间、年份间、基因型 × 年份间存在极显著差异。基于最新胡萝卜基因组数据构建了一张含有2 027个Bin(共154 776个SNP标记),总图距为1 436.433 cM,平均图距0.709 cM的遗传图谱。QTL定位分别获得了2、4、2个与始花期、主薹高和单株种子量相关位点,表型贡献率在14.6% ~ 23.8%之间,其中始花期位点DIF5-1和主薹高位点MSL5-1位置重叠。

关键词: 胡萝卜, 回交重组自交系, 始花期, 主薹高, 种子量, QTL

Abstract: A set of backcross inbred lines(BILs)crossed by a wild carrot species‘Songzi’with an orange cultivar‘Amsterdam’were used to deeply analyze the genetic influence of‘Songzi’chromosome fragments on date of initial flowering(DIF),main stalk length(MSL),and seed weight per plant(SWP). The experimental results suggested that MSL was normal distributed in 2014 and 2015,but DIF was on the contrary,and that SWP was only normal distributed in 2015. ANOVA analysis indicated that there was significant difference among BILs genotypes,years,and genotypes × years. Based on the last carrot genome resequencing data,a genetic map consisted of 2 027 Bins and 154 776 SNPs was developed,and the total genetic distance was 1 436.433 cM with an average interval of 0.709 cM between Bin markers. Two,four and two QTLs associated with DIF,MSL and SWP were discovered,respectively,with 14.6%–23.8% phenotypic variance. DIF5-1 locus was overlapped with MSL5-1 locus.

Key words: carrot, BIL, date of initial flowering, main stalk length, seed weight, QTL