园艺学报 ›› 2018, Vol. 45 ›› Issue (4): 717-724.doi: 10.16420/j.issn.0513-353x.2017-0375

• 研究论文 • 上一篇    下一篇


马 杰,徐婷婷,苏江硕,杨信程,房伟民,陈发棣,张 飞*   

  1. 南京农业大学园艺学院,南京 210095
  • 出版日期:2018-04-25 发布日期:2018-04-25
  • 基金资助:


Genetic Variation and QTL Mapping for Cold Tolerance of Ray Florets in an F1 Population of Chrysanthemum morifolium

MA Jie,XU Tingting,SU Jiangshuo,YANG Xincheng,FANG Weimin,CHEN Fadi,and ZHANG Fei*   

  1. College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China
  • Online:2018-04-25 Published:2018-04-25


以菊花‘南农雪峰’ב蒙白’的F1代为材料,利用电导率结合Logistic方程计算盛花期舌状花的低温半致死温度(LT50),分析其耐寒性的遗传变异,在此基础上开展QTL定位研究。结果表明,该F1群体舌状花的LT50在﹣8.92 ~ 1.31 ℃之间,变异系数为43.60%,近似正态分布,为多基因控制的数量性状,且存在一定程度的偏母性遗传和超亲分离现象。主基因 + 多基因混合遗传模型分析表明,该F1群体舌状花耐寒性无主基因控制。复合区间作图法共检测到6个QTL与菊花舌状花的耐寒性显著相关,分布在‘南农雪峰’遗传图的X2、X4连锁群和‘蒙白’遗传图的M2、M11、M33连锁群上,LOD值介于2.61 ~ 3.29之间,加性效应为﹣1.67 ~ 1.78 ℃,单个QTL可以解释耐寒性变异的贡献率为6.01% ~ 9.94%,均为微效QTL。

关键词: 菊花, 舌状花, 耐寒性, 遗传变异, QTL定位


In the present study,the relative electric conductivity and logistic equation were used to determine the low semi-lethal temperature(LT50)of ray floret in a segregating chrysanthemum‘Nannong Xuefeng’בMonalisa White’F1 population at full flowering stage,with the intention of investigating genetic variation and QTL for ray florets’cold tolerance. The results showed that LT50 ranged between–8.92 and 1.31 ℃ and distributed normally with a coefficient of variation of 43.60%,indicative of the quantitative nature;in addition,partial maternal inheritance to some extent and transgressive segregations were observed for the cold tolerance of ray florets in the F1 population. Major gene plus polygene inheritance model analysis figured out no major gene for the ray florets’cold tolerance. QTL analysis by the method of composite interval mapping identified six QTLs for ray florets’cold tolerance,harboring on the linkage group X2 and X4 of‘Nannong Xuefeng’map and M2,M11,and M33 of‘Monolisa White’map,with a LOD value of 2.61–3.29. The additive effect of the six QTLs varied in a range of–1.67–1.78 ℃,and the individually explained phenotypic variation ranged from 6.01% to 9.94%,therefore the identified QTLs should be minor genes,confirming the results of major gene plus polygene inheritance model analysis.

Key words: chrysanthemum, ray floret;cold tolerance, genetic variation, QTL mapping