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园艺学报 ›› 2017, Vol. 44 ›› Issue (4): 644-652.doi: 10.16420/j.issn.0513-353x.2016-0639

• 研究论文 • 上一篇    下一篇

桃PpSnRK1α在番茄中过表达对营养胁迫下植株生长的影响

罗静静1,张亚飞1,彭 妍2,彭福田1,*,赵永飞1,于 雯1   

  1. (1山东农业大学园艺科学与工程学院,作物生物学国家重点实验室,山东泰安 271018;2华中农业大学植物科学技术学院,武汉 430070)
  • 出版日期:2017-04-25 发布日期:2017-04-25

Effects of Overexpressing PpSnRK1α on the Growth of Tomato Plants Under Nutrient Stress

LUO Jingjing1,ZHANG Yafei1,PENG Yan2,PENG Futian1,*,ZHAO Yongfei1,and YU Wen1   

  1. (1College of Horticulture Science and Engineering,Shandong Agricultural University,State Key Laboratory of Crop Biology,Tai’an,Shandong 271018,China;2College of Plant Science,Huazhong Agricultural University,Wuhan 430070,China)
  • Online:2017-04-25 Published:2017-04-25

摘要:

以番茄(Solanum lycopericum)超表达桃SnRK1(蔗糖非发酵蛋白激酶–1)基因PpSnRK1α的株系及野生型为试材,研究在养分供应不足时SnRK1对植株生长的影响。结果表明,低营养条件下,转基因番茄叶片和根系中的SnRK1酶活性比野生型高41.55%和39.46%;功能叶片的净光合速率平均比野生型高18.98%;低营养胁迫12 d的叶片SOD、POD、CAT活性比野生型高35.56%、28.85%和14.90%;根系活力比野生型高26.39%;茎和叶中氮磷含量显著高于野生型,钾含量两者差别不大,在根系中氮磷含量差别不大,而钾含量显著高于野生型,且氮素向地上部茎和叶中的分配比率增加。上述结果说明,在营养缺乏条件下,超表达PpSnRK1α可以提高番茄功能叶净光合速率,促进植株对氮素的吸收利用,从而延缓叶片衰老。

关键词: 桃, SnRK1, 营养胁迫, 番茄, 净光合速率, 养分含量, 抗氧化酶

Abstract:

PpSnRK1α-overexpressing tomato(T2-3)and wild type tomato were used to study the effects of SnRK1 on the growth of tomato plants under nutrient stress. The results shows that:compared with wild type tomato,SnRK1 activity in leaves and roots increased by 41.55% and 39.46% of PpSnRK1α-overexpressing tomato under nutrient stress. The average photosynthetic rates increased by 18.98% in mature leaves of T2-3 compared with wild type. After 12 days under low nutrient stress,the transgenic lines overexpressing PpSnRK1α showedbetter antioxidant enzymes and root activity. The SOD,POD,CAT activities and root activity were increased 35.56% 8.85% 14.90% and 26.39% compared with wild type. The content of N,P in the stem and leaf of T2-3 was higher than that of wild type plants,while there was no obvious difference in the K content. In the roots,there were no significant difference in  

the N,P content,while the content of K of transgenic line is obviously higher than that of wild type. In addition,more N was distributed to aboveground parts. These results above show that overexpressing PpSnRK1α can improve the photosynthetic rates and promote the absorption of nitrogen,and then delay the leaf senescence.

Key words: peach, SnRK1, nutrient stress, tomato, photosynthetic rate, nutrient content, antioxidant enzyme

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