关键词: 大白菜, 结球甘蓝, 二体异附加系, InDel标记, 染色体

Abstract:

Based on the identification of Chinese cabbage–head cabbage addition lines and the screening of InDel markers on the corresponding linkage groups，46 selfing offspring individuals of Chinese cabbage - head cabbage chromosome 1 disomic alien addition lines were identified and analyzed using InDel markers and cytological method in order to clarify their chromosomal organization and number. The results showed that the specific fragment of head cabbage C03 chromosome could be amplified by each of 67 InDel markers corresponding to C03 linkage group of head cabbage in one individual plant at least. Of 46 selfing offsprings，three individual plants with 22 chromosomes were Chinese cabbage–head cabbage chromosome 1 disomic alien addition lines，six individual plants with 21 chromosomes were Chinese cabbage–head cabbage monosomic addition lines，one individual plant with20 chromosomes was Chinese cabbage–head cabbage disomic substitution line，twenty-eight individual plants with 20 chromosomes were Chinese cabbage translocation lines adding chromosomal C03 fragments of head cabbage. In addition，there were three individual plants with 22 chromosomes and two individual plants with 21 chromosomes，which were inferred to be Chinese cabbage aneuploids with the translocation fragments of head cabbage C03 chromosome. And three individual plants were not detected the specific bands of cabbage. This study will offer useful information for further research and utility of these selfing offsprings.

Key words: Chinese cabbage, head cabbage, disomic alien addition line, InDel markers, chromosome