Differences in the Pericarp Structure and the Expression of Expansin Genes After Irrigation Between Easily Cracked and Resistant Jujube

doi:10.16420/j.issn.0513-353x.2020-0903

Acta Horticulturae Sinica ›› 2021, Vol. 48 ›› Issue (9): 1785-1793.doi: 10.16420/j.issn.0513-353x.2020-0903

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Differences in the Pericarp Structure and the Expression of Expansin Genes After Irrigation Between Easily Cracked and Resistant Jujube

XIN Haiqing¹^,², ZHOU Junyong¹^,², SUN Yaoxing¹^,², MU Wenlei², YANG Jian², MA Fuli², SUN Jun², XUE Zhengrong², LU Lijuan¹, SUN Qibao¹^,^**()

¹Horticulture Research Institute,Anhui Academy of Agricultural Sciences,Hefei 230031,China
²Horticulture College,Anhui Agricultural University,Hefei 230036,China

Received:2021-06-01 Revised:2021-08-17 Online:2021-09-25 Published:2021-09-30
Contact: SUN Qibao E-mail:anhuisqb@163.com

Abstract

Abstract:

To understand the cracking-resistant mechanism of Ziziphus jujuba,the crisp mature fruits of the cracking-resistant‘Wanzao 3’and cracking-susceptible‘Lifu Gongzao’were used as materials to measure fruit firmness and pericarp microstructure in the shoulder,equator and top,and to detect the expression levels of expansin genes in shoulder pericarp of the two cultivars under irrigation using real time PCR,respectively. The results showed that the shoulder of ‘Lifu Gongzao’was cracked after irrigation 30 h. The fruit firmness of the shoulder,equator,and top of‘Wanzao 3’was significantly higher than that of‘Lifu Gongzao’respectively,and the firmness of the equator and top of‘Lifu Gongzao’was significantly higher than that of the shoulder under irrigation. Pericarp cells of‘Wanzao 3’were arranged more tightly and regularly than those of‘Lifu Gongzao’,and pericarp cells of the equator and top of‘Lifu Gongzao’were arranged more tightly and regularly than the shoulder under irrigation. Pericarp thickness of the equator and top of‘Lifu Gongzao’was more than that in the shoulder,however,the increased thickness of the equator and top was less than that of the shoulder under irrigation. The expression levels of EXPA genes ZjEXPA4,ZjEXPA5-like,ZjEXPA6,ZjEXPA14,and ZjEXPA17 in the shoulder pericarp of‘Wanzao 3’were higher than those of‘Lifu Gongzao’under irrigation.

Key words: Ziziphus jujuba, cracking, pericarp structure, expansin

CLC Number:

S665.1

XIN Haiqing, ZHOU Junyong, SUN Yaoxing, MU Wenlei, YANG Jian, MA Fuli, SUN Jun, XUE Zhengrong, LU Lijuan, SUN Qibao. Differences in the Pericarp Structure and the Expression of Expansin Genes After Irrigation Between Easily Cracked and Resistant Jujube[J]. Acta Horticulturae Sinica, 2021, 48(9): 1785-1793.

Figures/Tables 6

References 23

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基因 Gene	上游引物（5′-3′） Forward primer	下游引物（5′-3′） Reverse primer
ZjEXPA4	GGCTATTTTGCCACCACAG	GCCAGAGCTTGTCTTTCCA
ZjEXPA5-like	CAACAACAGCAAATGGAAA	CAGACATACACTGGAAGCC
ZjEXPA6	TCTCACCCCACAAACAACG	GGACAGAACTGGCAGAGCA
ZjEXPA14	GTCGCTGGTAGTGACCTTGA	GGGAGAAGAGGAGGCATAAG
ZjEXPA17	GTGGCAGTGACGAGAATGGA	GCCTCTGGAACTATGGGTGG
ZjEXLB7	CAGATGAAGAATAACGGGAGC	TATGGTGCAGTATGGACAACG
ZjACTIN1	AGCCTTCCTGCCAACGAG	TTGCTTCTCACCCTTGATGC

基因 Gene	上游引物（5′-3′） Forward primer	下游引物（5′-3′） Reverse primer
ZjEXPA4	GGCTATTTTGCCACCACAG	GCCAGAGCTTGTCTTTCCA
ZjEXPA5-like	CAACAACAGCAAATGGAAA	CAGACATACACTGGAAGCC
ZjEXPA6	TCTCACCCCACAAACAACG	GGACAGAACTGGCAGAGCA
ZjEXPA14	GTCGCTGGTAGTGACCTTGA	GGGAGAAGAGGAGGCATAAG
ZjEXPA17	GTGGCAGTGACGAGAATGGA	GCCTCTGGAACTATGGGTGG
ZjEXLB7	CAGATGAAGAATAACGGGAGC	TATGGTGCAGTATGGACAACG
ZjACTIN1	AGCCTTCCTGCCAACGAG	TTGCTTCTCACCCTTGATGC

灌水后时间/h Time after irrigation	果肩 Shoulder		赤道 Equator		果顶 Top
灌水后时间/h Time after irrigation	李府贡枣 Lifu Gongzao	皖枣3号 Wanzao 3	李府贡枣 Lifu Gongzao	皖枣3号 Wanzao 3	李府贡枣 Lifu Gongzao	皖枣3号 Wanzao 3
0	6.81 ± 0.34 D	10.12 ± 0.15 AB	8.57 ± 0.36 D	11.93 ± 0.27 A	7.86 ± 0.47 B	10.76 ± 0.43 A
6	5.77 ± 0.28 DE	9.10 ± 0.37 BC	6.70 ± 0.24 E	10.52 ± 0.50 ABC	6.27 ± 0.19 D	9.64 ± 0.39 A
12	5.50 ± 0.28 E	8.39 ± 0.37 C	7.17 ± 0.27 E	9.93 ± 0.38 C	6.40 ± 0.23 CD	7.73 ± 0.38 BC
18	5.84 ± 0.17 DE	10.31 ± 0.22 A	7.34 ± 0.27 DE	11.47 ± 0.45 AB	6.81 ± 0.26 BCD	9.72 ± 0.37 A
24	5.51 ± 0.39 E	10.01 ± 0.20 AB	6.91 ± 0.38 E	10.79 ± 0.18 ABC	6.89 ± 0.24 BCD	9.87 ± 0.23 A
30	4.92 ± 0.27 E	10.67 ± 0.20 A	6.15 ± 0.34 E	10.15 ± 0.23 BC	6.58 ± 0.23 BCD	9.83 ± 0.24 A

灌水后时间/h Time after irrigation	果肩 Shoulder		赤道 Equator		果顶 Top
灌水后时间/h Time after irrigation	李府贡枣 Lifu Gongzao	皖枣3号 Wanzao 3	李府贡枣 Lifu Gongzao	皖枣3号 Wanzao 3	李府贡枣 Lifu Gongzao	皖枣3号 Wanzao 3
0	6.81 ± 0.34 D	10.12 ± 0.15 AB	8.57 ± 0.36 D	11.93 ± 0.27 A	7.86 ± 0.47 B	10.76 ± 0.43 A
6	5.77 ± 0.28 DE	9.10 ± 0.37 BC	6.70 ± 0.24 E	10.52 ± 0.50 ABC	6.27 ± 0.19 D	9.64 ± 0.39 A
12	5.50 ± 0.28 E	8.39 ± 0.37 C	7.17 ± 0.27 E	9.93 ± 0.38 C	6.40 ± 0.23 CD	7.73 ± 0.38 BC
18	5.84 ± 0.17 DE	10.31 ± 0.22 A	7.34 ± 0.27 DE	11.47 ± 0.45 AB	6.81 ± 0.26 BCD	9.72 ± 0.37 A
24	5.51 ± 0.39 E	10.01 ± 0.20 AB	6.91 ± 0.38 E	10.79 ± 0.18 ABC	6.89 ± 0.24 BCD	9.87 ± 0.23 A
30	4.92 ± 0.27 E	10.67 ± 0.20 A	6.15 ± 0.34 E	10.15 ± 0.23 BC	6.58 ± 0.23 BCD	9.83 ± 0.24 A

灌水后时间/h Time after irrigation	李府贡枣 Lifu Gongzao				皖枣3号 Wanzao 3
	李府贡枣 Lifu Gongzao				皖枣3号 Wanzao 3			果肩 Shoulder	赤道 Equater	果顶 Top	果肩 Shoulder	赤道 Equater	果顶 Top
	0	51.5 ± 0.8 C	61.5 ± 1.1 B	76.6 ± 2.1 A		75.3 ± 1.3 C	75.5 ± 1.4 B	73.9 ± 1.5 B
6	71.6 ± 3.5 B	75.7 ± 1.3 A	80.6 ± 1.8 A		79.3 ± 1.5 BC	76.0 ± 1.8 BC	78.6 ± 0.7 AB
12	75.2 ± 1.4 AB	81.5 ± 2.3 A	80.8 ± 2.5 A		84.3 ± 1.2 AB	85.2 ± 1.9 A	80.1 ± 1.4 A
18	82.3 ± 2.1 A	81.7 ± 2.3 A	82.6 ± 1.7 A		87.1 ± 0.7 A	84.6 ± 1.3 A	81.1 ± 1.1 A
24	82.8 ± 0.7 A	80.7 ± 1.2 A	80.3 ± 2.6 A		88.3 ± 1.1 A	85.1 ± 0.7 A	82.6 ± 0.8 A
30	56.0 ± 1.6 C	77.6 ± 1.6 A	80.4 ± 2.9 A		89.1 ± 1.0 A	82.3 ± 1.3 AB	81.7 ± 0.6 A

Differences in the Pericarp Structure and the Expression of Expansin Genes After Irrigation Between Easily Cracked and Resistant Jujube

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