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园艺学报 ›› 2019, Vol. 46 ›› Issue (10): 1919-1935.doi: 10.16420/j.issn.0513-353x.2018-0743

• 研究论文 • 上一篇    下一篇

香蕉Aux/IAA基因家族的全基因组鉴定及表达分析

孙雪丽,刘 范,田 娜,项蕾蕾,郝向阳,王 云,彭丽云,王天池,程春振*,赖钟雄*   

  1. 福建农林大学园艺学院,园艺植物生物工程研究所,福州 350002
  • 出版日期:2019-10-25 发布日期:2019-10-25
  • 基金资助:
    国家自然科学基金项目(31601713);福建农林大学科技创新专项基金项目(CXZX2018082);国家现代农业产业技术体系建设专项资金项目(CARS-32-11);福建农林大学“校杰出青年科研人才”计划项目(xjq201721);福建省高原学科建设经费项目(102/71201801101)

Genome-wide Identification and Expression Analysis of Aux/IAA Gene Family in Banana

SUN Xueli,LIU Fan,TIAN Na,XIANG Leilei,HAO Xiangyang,WANG Yun,PENG Liyun,WANG Tianchi,CHENG Chunzhen*,and LAI Zhongxiong*   

  1. College of Horticulture,Institute of Horticultural Biotechnology,Fujian Agriculture and Forestry University,Fuzhou 350002,China
  • Online:2019-10-25 Published:2019-10-25

摘要: 对香蕉Aux/IAA基因家族进行了全基因组鉴定,获得分布于除1号染色体外的10条染色体上的44个家族成员,其CDS长度为378 ~ 1 062 bp,其中10个存在选择性剪切体。它们编码的蛋白包含125 ~ 353个氨基酸,均为不含信号肽的亲水蛋白。序列分析结果表明:该基因家族成员多为5个外显子和4个内含子的基因结构,编码蛋白多具有4个典型的保守基序。亚细胞定位预测结果显示,Aux/IAA成员多定位于细胞核、细胞质和叶绿体,定位情况与保守结构域有一定相关性。MaIAA31是该家族中唯一预测有跨膜区域且亚细胞观察定位在胞外的成员。进化树分析结果显示,香蕉Aux/IAA可分为2大类8个亚组。顺式作用元件分析结果显示该家族成员启动子包含激素、光、非生物以及生物胁迫响应相关元件。通过分析部分成员在不同激素和逆境处理下的表达情况发现:MaIAA基因的表达受多因素调控,其中MaIAA27变化显著,MaIAA15响应干旱和盐胁迫,MaIAA25在热、低温和机械损伤胁迫下显著上调,暗示它们在香蕉响应这些逆境的过程中发挥作用。

关键词: 香蕉, Aux/IAA基因家族, 生物信息学, 基因表达

Abstract: In this study,the banana Aux/IAA gene family members were screened and identified from genome level using bioinformatic methods. A total of 44 Aux/IAA family members distributed in all the banana chromosomes except chromosome 1 were identified,among which 10 members own alternative splicing variants. The coding sequence of these genes ranges from 378 to 1 062 bp,encoding proteins with 125–353 aa. All of their encoded protein were hydrophilic proteins without signal peptide. Gene structure analysis showed that most members of the family shared 5 exons and 4 introns,and most of their encoded proteins contained 4 typical conserved motifs. Subcellular location prediction results showed that the Aux/IAA members were mainly located in nucleus,cytoplasm and chloroplast. MaIAA31 is the only member of the family that predicted a transmembrane region and was located outside the extracellular. Phylogenetic tree analysis revealed that the family members could be divided into two major categories and eight subgroups. Promoter cis-acting elements prediction analysis identified several elements responsive to phytohormone and light,abiotic and biotic stresses. Gene expression analysis of several Aux/IAA members under different phytohormone and stress treatments revealed that the expression of Aux/IAAs were regulated by multiple factors. Among these investigated genes,the expression of MaIAA27 was found to be responsive to all the treatments,MaIAA15 was influenced by drought and salt,while MaIAA25 was greatly induced by heat,low-temperature and wound,indicating that they might contribute to the stress responses of banana.

Key words: banana, Aux/IAA gene family, bioinformatic analysis, gene expression

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