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园艺学报 ›› 2019, Vol. 46 ›› Issue (7): 1379-1387.doi: 10.16420/j.issn.0513-353x.2018-0518

• 研究报告 • 上一篇    下一篇

苦参碱对老龄苹果园土盆栽平邑甜茶和土壤微生物的影响

王 柯1,王艳芳1,陈学森1,公维松2,沈 向1,尹承苗1,*,毛志泉1,*   

  1. 1山东农业大学园艺科学与工程学院,作物生物学国家重点实验室,山东泰安 271018;2临沂蒙山旅游度假区农林水利局,山东临沂 273304
  • 出版日期:2019-07-25 发布日期:2019-07-25
  • 基金资助:
    现代农业产业技术体系建设专项资金项目(CARS-27);国家自然科学基金项目(31672104,31501720);国家重点研发计划项目(2016YFD0201114);山东省水果创新团队项目(SDAIT-06-07)

Effects of Matrine on Old Apple Orchard Soil Potted Malus hupehensis and Soil Microorganisms

WANG Ke1,WANG Yanfang1,CHEN Xuesen1,GONG Weisong2,SHEN Xiang1,YIN Chengmiao1,*,and MAO Zhiquan1,*   

  1. 1College of Horticultural Science and Engineering,Shandong Agricultural University,State Key Laboratory of Crop Biology,Tai’an,Shandong 271018,China;2Linyi Mengshan Tourism and Resort Bureau,Linyi,Shandong 273304,China
  • Online:2019-07-25 Published:2019-07-25

摘要: 以老龄苹果园土盆栽的平邑甜茶幼苗为试材,研究向土壤中添加不同量的苦参碱对幼苗生长以及土壤中微生物数量的影响。结果表明,9月时,与对照相比,向土壤中添加0.01%苦参碱处理可明显提高平邑甜茶幼苗株高、地径、干质量和根表面积、根体积以及根呼吸速率,根系SOD、POD和CAT活性也显著提高。经苦参碱处理后,土壤中细菌和真菌数量减少,真菌比细菌降幅更大,细菌/真菌比值增大。其中0.01%苦参碱处理的细菌和真菌分别比对照减少了58.5%和68.3%,细菌/真菌比值比对照增加30.6%,土壤中微生物多样性指数(Shannon index)和丰富度指数(Margalef index)降低显著,腐皮镰孢菌、层出镰孢菌、串珠镰孢菌和尖孢镰孢菌基因拷贝数分别比对照降低了51.2%、43.3%、40.0%和44.5%。综上,0.01%苦参碱处理促进了平邑甜茶幼苗的生长,改善了土壤环境,可有效减轻平邑甜茶的连作障碍。

关键词: 平邑甜茶, 老龄苹果园, 连作障碍, 土壤, 苦参碱

Abstract: Old apple orchard with soil potted Malus hupehensis Rehd. seedlings as test material,the effects of matrine on seedling growth and soil microbial population were studied. The results showed that in September,compared to the control group,adding 0.01% matrine to the soil can significantly improve plant height,ground diameter,dry weight,root surface area,root volume and root respiration rate of Malus hupehensis Rehd. seedlings,and the activities of root SOD,POD and CAT were also significantly improved. After matrine treatment,the number of bacteria and fungi in soil decreased,the decline of fungi was greater than that of bacteria,and the ratio of bacteria to fungi increased. The bacteria and fungi treated with 0.01% matrine decreased by 58.5% and 68.3%,respectively,and the ratio of bacteria to fungi increased by 30.6%. The Shannon index and Margalef index of microbial diversity in soil treated with matrine were significantly reduced,and the gene copy numbers of Fusarium solani,Fusarium proliferatum,Fusarium moniliforme and Fusarium oxysporum were reduced by 51.2%,43.3%,40.0% and 44.5%,respectively,compared with the control. In conclusion,0.01% matrine treatment promoted the growth of Malus hupehensis seedlings,improved the soil environment,and effectively alleviated the replant disease of Malus hupehensis.

Key words: Malus hupehensis, old apple orchard, replant disease, soil, matrine

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