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园艺学报 ›› 2019, Vol. 46 ›› Issue (7): 1238-1248.doi: 10.16420/j.issn.0513-353x.2018-0688

• 研究论文 • 上一篇    下一篇

两株植物内生拮抗菌对连作土盆栽平邑甜茶幼苗生长及土壤酶活性的影响

刘丽英1,*,许 超1,*,刘珂欣1,迟晓丽1,朱 浩1,张 潇1,金 晓1,刘维维1,孙中涛1,**,毛志泉2,**   

  1. 1山东农业大学生命科学学院,山东泰安 271018;2山东农业大学园艺科学与工程学院,作物生物学国家重点试验室,山东泰安 271018
  • 出版日期:2019-07-25 发布日期:2019-07-25
  • 基金资助:
    国家现代农业产业技术体系建设专项资金项目(CARS-28);山东省重点研发计划项目(2017CXGC0306);济南市科学技术发展项目(201704026);济南市西部经济隆起带人才项目

Effects of Two Endophytic Antagonistic Bacteria on Growth of Pot Experiment Malus hupehensis Seedlings and the Soil Enzymes Activity Under Continuous Cropping Conditions

LIU Liying1,*,XU Chao1,*,LIU Kexin1,CHI Xiaoli1,ZHU Hao1,ZHANG Xiao1,JIN Xiao1,LIU Weiwei1,SUN Zhongtao1,**,and MAO Zhiquan2,**   

  1. 1College of Life Science,Shandong Agricultural University,Tai’an,Shandong 271018,China;2College of Horticulture Science and Engineering,State Key Laboratory of Crop Biology,Shandong Agricultural University,Tai’an,Shandong 271018,China
  • Online:2019-07-25 Published:2019-07-25

摘要: 为研发对苹果连作障碍有防治作用的微生物菌剂,以6种常见病原菌为供试病原菌,以连作苹果园中健康苹果树根部为材料,采用对峙法对内生拮抗菌进行筛选,获得了两株对病原菌有明显拮抗效果的内生拮抗细菌SRB-1和SRB-11。鉴定发现,SRB-1和SRB-11分别为枯草芽孢杆菌Bacillus subtilis和漠海威芽孢杆菌Bacillus mojavensis。通过苹果连作土盆栽平邑甜茶试验对菌株SRB-1和SRB-11菌肥防治连作障碍的效果进行验证,结果表明,连作土中施加菌肥对平邑甜茶幼苗生长有明显促生作用。与对照相比,株高、地径、鲜样质量和干样质量显著增加,根系超氧化物歧化酶(SOD)、过氧化氢酶(CAT)以及过氧化物酶(POD)活性分别增加了65%、51%和230%;同时土壤中的蔗糖酶、脲酶、磷酸酶和过氧化氢酶的活性分别提高了132%、57%、82%和146%。表明本研究中筛选的内生拮抗菌SRB-1和SRB-11所制成的微生物菌剂对苹果连作障碍有一定的防治作用。

关键词: 平邑甜茶, 连作障碍, 内生拮抗菌, 促生作用, 土壤酶活性, 根系酶活性

Abstract: In order to develop a microbial agent that can prevent and cure continuous cropping obstacles in apple,six common pathogens were tested,and the roots of healthy apple in continuous cropping orchard were used as materials to screen the antagonistic endophytes. Two strains SRB-1 andSRB-11 with significant antagonism were identified as Bacillus subtilis and Bacillus mojavensis,respectively. The pot experiment was carried out to verify the effect of SRB-1 and SRB-11 on the continuous cropping obstacle of apple. The results of pot experiment showed that the application of bacterium fertilizer in soil significantly promoted the growth of Malus hupehensis seedlings under continuous cropping conditions and had a good effect on the soil. Compared with control treatment,the application of bacterium fertilizer significantly increased plant height,basal diameter,fresh weight and dry weight,increased the activity of superoxide dismutase(SOD),catalase(CAT)and peroxidase(POD)in the roots of Malus hupehensis by 65%,51% and 230%,respectively. Meanwhile,the activities of invertase,urease,phosphatase and catalase in soil were significantly increased by 132%,57%,82% and 146%,respectively. The above results indicate that the microbial agents prepared by the endogenous antagonistic SRB-1 and SRB-11 screened in this study have a certain preventive effect on apple continuous cropping disorders.

Key words: Malus hupehensis, continuous cropping obstacle, endophyte antagonism, promoting effect, soil enzyme activity, root enzyme activity

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