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园艺学报 ›› 2015, Vol. 42 ›› Issue (10): 2049-2058.doi: 10.16420/j.issn.0513-353x.2015-0338

• 研究报告 • 上一篇    下一篇

茄子3个Aux/IAA基因的克隆及表达分析

张立慧1,*,田时炳2,*,王志敏1,**,安礼渝1,汤青林1,王永清2,杨 洋2,宋 明1,**   

  1. 1西南大学园艺园林学院,南方山地园艺学教育部重点实验室,重庆市蔬菜学重点实验室,重庆 400715;2重庆市农业科学院蔬菜花卉研究所,重庆 400055
  • 出版日期:2015-10-25 发布日期:2015-10-25
  • 基金资助:

    国家自然科学基金项目(31501756);国家现代农业产业技术体系建设专项资金项目(CARS-25-A-06);中央高校基本科研
    业务费专项(XDJK2014C092)

Gene Cloning and Expression Analysis of Three Aux/IAA Genes in Eggplant

ZHANG Li-hui1,*,TIAN Shi-bing2,*,WANG Zhi-min1,**,AN Li-yu1,TANG Qing-lin1,WANG Yong-qing2,YANG Yang2,and SONG Ming1,**   

  1. 1College of Horticulture and Landscape Architecture,Southwest University,Key Laboratory of Horticulture Science for Southern Mountainous Regions,Ministry of Education,Chongqing Key Laboratory of Olericulture,Chongqing 400715,China;2The Institute of Vegetables and Flowers,Chongqing Academy of Agricultural Sciences,Chongqing 400055,China
  • Online:2015-10-25 Published:2015-10-25

摘要:

以茄子单性结实品系‘D-7-1’为材料,克隆到3个新的Aux/IAA基因,分别命名为SmIAA3、SmIAA4和SmIAA9,其CDS长度分别为573、564和915 bp,分别编码190、187和304个氨基酸;系统进化树分析表明,3个蛋白均属于Aux/IAA家族蛋白,与番茄亲缘关系较近,且均具有Aux/IAA家族蛋白的DomainⅠ、Ⅱ、Ⅲ 和 Ⅳ 保守结构域;其分子量分别为21.50、20.95和32.67 kD,理论等电点分别为7.62、6.02和8.57。实时定量PCR分析表明:SmIAA9基因在单性结实和非单性结实品系茄子中的表达量差异明显,开花前单性结实品系的表达量显著高于非单性结实品系,推测其与茄子单性结实相关。

关键词: 茄子, 单性结实, Aux/IAA, 克隆, 生物信息学, 表达分析

Abstract:

Three Aux/IAA genes SmIAA3,SmIAA4 and SmIAA9 in eggplant were cloned from parthenocarpic eggplant‘D-7-1’. The full CDS length of SmIAA3,SmIAA4 and SmIAA9 was 573,564 and 915 bp,which encoded three polypeptides of 190,187 and 304 amino acids. The phylogenetic analysis showed that the three proteins belonged to Aux/IAA family proteins and they had the highest similarity with Aux/IAA proteins of tomato. And the three novel proteins contained conserved domainⅠ,Ⅱ,Ⅲ and Ⅳ of Aux/IAA family proteins. The molecular weight of the three proteins were 21.50,20.95 and 32.67 kD with isoelectric point 7.62,6.02 and 8.57 respectively. The RT-PCR analysis showed that the expression of SmIAA9 gene was different between parthenocarpic and nonparthenocarpic eggplant. Evidently the parthenocarpic was higher than the nonparthenocarpic before flowering,which indicated thatSmIAA9 would be a gene related with parthenocarpy.

Key words: eggplant, parthenocarpy, Aux/IAA, cloning, bioinformaticanalysis, expression analysis

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