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园艺学报 ›› 2015, Vol. 42 ›› Issue (10): 1869-1878.doi: 10.16420/j.issn.0513-353x.2015-0126

• 果树 •    下一篇

不同溶质桃果实的软化与乙烯合成相关基因的差异表达

杨 勇1,2,马瑞娟1,张斌斌1,宋志忠1,张春华1,郭绍雷1,俞明亮1,2,*   

  1. 1江苏省农业科学院园艺研究所,江苏省高效园艺作物遗传改良重点实验室,南京 210014;2南京农业大学园艺学院,南京 210095
  • 出版日期:2015-10-25 发布日期:2015-10-25
  • 基金资助:

    江苏省农业科技自主创新资金项目[CX(14)2015];国家现代农业产业技术体系建设专项资金项目(CARS-31)

Differential Expression Analysis in Fruit Softening and Ethylene Biosynthetic Pathways in Peaches of Different Flesh Textures

YANG Yong1,2,MA Rui-juan1,ZHANG Bin-bin1,SONG Zhi-zhong1,ZHANG Chun-hua1,Guo Shao-lei1,and YU Ming-liang1,2,*   

  1. 1Institute of Horticulture,Jiangsu Academy of Agricultural Sciences,Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement,Nanjing 210014,China;2College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China
  • Online:2015-10-25 Published:2015-10-25

摘要:

以八成熟Stonyhard型桃‘霞脆’和‘有名白桃’、软溶质桃‘银花露’、硬溶质桃‘湖景蜜露’、不溶质桃‘金童6号’的果实为试材,研究了25 ℃和4 ℃贮藏条件下果实软化及乙烯生物合成途径相关基因表达水平的差异。结果显示:两种贮藏温度下,Stonyhard型桃‘有名白桃’和‘霞脆’果实释放极少量乙烯;25 ℃常温条件下,Stonyhard型桃果实硬度保持较高水平,但4 ℃低温诱导了‘有名白桃’果实软化。实时荧光定量PCR表明,软溶质、硬溶质和不溶质桃的PpACS1基因均具有高表达丰度,而Stonyhard型‘霞脆’和‘有名白桃’的表达水平极低;两种贮藏温度下,5个桃品种果实在整个贮藏期间均未检测到PpACS2和PpACS3基因的表达。此外,低温诱导了PpACO1基因在Stonyhard型桃‘霞脆’和‘有名白桃’中的表达,‘有名白桃’果实endo-PG基因受低温刺激表达也上调。说明不同肉质类型的桃贮藏期间果实软化与乙烯的生物合成关系密切,不同温度下通过调节相关基因的表达水平进而调控果实软化进程。

关键词: 桃, 乙烯, 生物合成途径, 软化, 基因表达

Abstract:

To study the expression difference of genes involved in fruit softening and ethylene biosynthetic pathways under different temperature storage,different peach cultivars of‘Xiacui’and‘Yumyeong’with stonyhard,‘Yinhualu’with soft-melting,‘Hujing Milu’with hard-melting,and‘Babygold 6’with non-melting in 8 tenth maturity were collected as test materials. The results showed that only a little ethylene production were detected after harvest with fruits of‘Yumyeong’and‘Xiacui’,under either room temperature(25 ℃)or low temperature(4 ℃). The fruit firmness of stonyhard cultivars retain at high level under room temperature with time going on,whereas low temperature induced‘Yumyeong’fruit to soften. Real-time quantitative PCR results indicated that PpACS1 gene was highly expressed in soft-melting,hard-melting and non-melting cultivars,but extremely low in stonyhard peaches. However,PpACS2 or PpACS3 could not be detected in all five cultivars. Moreover,cold treatment induced the expression level of PpACO1 gene in‘Xiacui’and‘Yumyeong’peaches,and significantly enhanced endo-PG expression in‘Yumyeong’peaches. In conclusion,this study revealed that fruit softening of peaches with different flesh textures were closely related to ethylene biosynthesis during the storage period,which were controlled via regulating relevant gene expression levels under different storage temperatures.

Key words: Prunus persica, ethylene, biosynthetic pathway, soften, gene expression

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