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园艺学报 ›› 2014, Vol. 41 ›› Issue (12): 2419-2426.

• 蔬菜 • 上一篇    下一篇

硅对干旱胁迫下番茄根系细胞超微结构及线粒体活性氧代谢的影响

曹逼力,刘灿玉,徐 坤   

  1. 山东农业大学园艺科学与工程学院,作物生物学国家重点实验室/农业部黄淮地区园艺作物生物学与种质创制重点实验室,山东泰安 271018
  • 出版日期:2014-12-25 发布日期:2014-12-25
  • 基金资助:
    山东省现代农业产业技术体系专项资金项目(SDAIT-02-022-05)

Effects of Silicon on Ultrastructure and Active Oxygen Metabolism in Mitochondria of Tomato Roots Under Drought Stress

CAO Bi-Li, LIU Can-Yu, XU Kun   

  1. College of Horticulture Science and Engineering,Shandong Agricultural University,State Key Laboratory of Crop Biology,Key Laboratory of Biology and Genetic Improvement of Horticultural Crops in Huang-Huai Region,Ministry of Agriculture,Tai’an,Shandong 271018,China
  • Online:2014-12-25 Published:2014-12-25

摘要: 以番茄品种‘金棚1号’为试材,采用水培方法,研究了PEG-6000模拟干旱胁迫条件下,硅对根系细胞超微结构及线粒体活性氧代谢的影响。结果表明,随干旱胁迫时间的延长,番茄植株根系活力逐渐降低,丙二醛(MDA)含量逐渐升高,细胞线粒体超氧阴离子生成速率和过氧化氢(H2O2)含量亦逐渐升高,而超氧化物歧化酶(SOD)、过氧化物酶(POD)及过氧化氢酶(CAT)活性在胁迫初期迅速增强,达峰值后又快速降低;施硅处理显著抑制了干旱胁迫引发的上述变化。干旱胁迫处理12 d时,施硅较不施硅处理番茄根系的MDA含量、超氧阴离子生成速率和H2O2含量分别降低了25.14%、8.34%和20.25%,而根系活力、SOD活性、POD活性和CAT活性分别提高了139.62%、243.19%、65.53%和125.55%。施硅处理还减轻了干旱胁迫导致的根系细胞质壁分离程度,缓解了线粒体、细胞核、生物膜等的损伤。

关键词: 番茄, 硅, 干旱, 细胞超微结构, 线粒体, 活性氧代谢

Abstract: The effects of silicon on ultrastructure and active oxygen metabolism in root mitochondria were studied with a tomato(Solanum lycopersicum)cultivar‘Jinpeng 1’in cultured hydroponics under PEG-6000 simulated drought. The results showed that with the extension of drought stress,the root activity of tomato plants gradually decreased. Malondialdehyde(MDA)content,mitochondrial superoxide anion()productivity rate and hydrogen peroxide(H2O2)content gradually increased,but the activities of superoxide dismutase(SOD),peroxidase(POD)and catalase(CAT)rapidly increased in the early stage of drought stress,and then rapidly decreased after peak. The application of silicon significantly effected the change of the related indicators caused by drought stress. After 12 days of drought stress,the content ofMDA,and H2O2 in roots of tomato with silicon were lower than that of tomato without silicon by 25.14%,8.34%,20.25%,respectively. But root activity,SOD activity,POD activity and CAT activity were higher by 139.62%,243.19%,65.53%,125.55%,respectively. In addition,the application of silicon not only reduced plasmolysis of tomato root cells,but also relieved the damage of mitochondria,nuclei and biofilms.

Key words: tomato, silicon, drought, cell ultrastructure, mitochondria, active oxygen metabolism

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