关键词: 茄子, 单性结实, 基因克隆, 表达分析

Abstract: A full length cDNA（800 bp）is cloned by RT-PCR and RACE from parthenocarpic fruit of eggplant（Solanum melongena）line D-10，based on the EST Z732 from eggplant parthenocarpic suppression subtractive hybridization library. The cloned gene belongs to IAA family gene（SmIAA19 accession number KP114221），which contains an open reading frame（558 bp）and encodes a protein of 185 amino acids. The result of amino acid sequence alignment shows that the protein posses typical structure domain and conservative basic sequence of Aux/IAA gene families. The gene is much more close to potatoes in the evolutionary tree，and on the same branch with tomatoes. Real-time PCR was used to analyze the expression pattern of SmIAA19 in different varieties and growing period. Result of qRT-PCR indicates that SmIAA19 gene is expressed in ovary and fruit of parthenocarpic and non parthenocarpic eggplant. The expression in parthenocarpy is significantly higher than that of non-parthenocarpy. The highest expression is measured at anthesis day in parthenocarpic ovaries. SmIAA19 expression quantity keeps at low level in the non-parthenocarpy strain neither the condition of low temperature nor theoptimum temperature. The test shows that the change trend of IAA content is basically the same during the development of eggplant fruit in both parthenocarpic fruits and unparthenocarpic fruits，and IAA content is higher at the early stage. The expression of SmIAA19 gene in the different eggplant strain is consistent with the change of IAA content under the condition of low temperature（the first inflorescence fruit）. So this research suggested that SmIAA19 gene may be associated with the eggplant parthenocarpy.

Key words: eggplant, parthenocarpy, gene cloning, expression analysis