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园艺学报 ›› 2012, Vol. 39 ›› Issue (11): 2151-2158.doi:

• 蔬菜 • 上一篇    下一篇

契斯曼尼番茄果实中可溶性固形物和β–胡萝卜素含量相关基因QTL 分析

 鲁亚辉, 杜永臣, 王孝宣, 高建昌, 国艳梅   

  1. (中国农业科学院蔬菜花卉研究所,北京100081)
  • 出版日期:2012-11-25 发布日期:2012-11-25

QTL Mapping of Soluble Solids Content and Beta-carotene in Solanum cheesmanii

LU  Ya-Hui, DU  Yong-Chen, WANG  Xiao-Xuan, GAO  Jian-Chang, GUO  Yan-Mei   

  1. (Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China)
  • Online:2012-11-25 Published:2012-11-25

摘要: 以野生番茄契斯曼尼LA0317与栽培番茄自交系9706为亲本,构建了包括152个株系的BC2S6群体。利用155个SSR标记、21个CAPS标记和88个Indel标记构建了契斯曼尼番茄的遗传连锁图谱,总图距1 134.0 cM,标记间平均距离4.29 cM。采用复合QTL区间作图法对番茄果实中的可溶性固形物含量和β–胡萝卜素含量进行QTL分析。定位到10个与可溶性固形物含量相关的QTLs,分别位于第3、4、5、6、9、10、11号染色体上,其中有5个QTL效应值在12%以上。定位到6个与β–胡萝卜素含量相关的QTLs,分别位于第1、2、6、9号染色体上。其中,位于6号染色体上的qBC6b效应值达到54.7%,为一主效QTL。此外,还获得了可用作育种材料的5个高可溶性固形物含量株系和8个高β–胡萝卜素含量的株系。

关键词: 番茄, 契斯曼尼, 可溶性固形物, β–胡萝卜素, QTL

Abstract: A population BC2S6 with 152 lines was developed from the cross between Solanum lycopersicum 9706 and Solanum cheesmanii LA0317. A linkage map was constructed with 155 SSR marker,21 CAPS marker and 88 Indel marker. The map covered 1 134.0 cM,and the average distance between adjacent loci was 4.29 cM.Using MQM-mapping method,QTL of soluble solids content(SSC)and Beta-carotene content were detected. Ten QTL of SSC were mapped to chromosome 3,4,5,6,9,10 and 11. Respectively,five of which explained more than 12% of the phenotypic variation. Six QTL of Beta-carotene content were mapped to chromosome 1,2,6,9. The major QTL of Beta-carotene content on chromosome 6 could explain 54.7% of the phenotypic variation.Moreover,5 lines with high SSC and 8 lines with high Beta-carotene content were obtained,and could be used as breeding materials.

Key words: tomato, Solanum cheesmanii, soluble solids content, Beta-carotene content, QTL

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