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园艺学报 ›› 2001, Vol. 28 ›› Issue (2): 161-163.

• 研究报告 • 上一篇    下一篇

脐橙果实发育过程中有机酸合成代谢酶活性的变化

文 涛1;熊庆娥1;曾伟光2;刘远鹏2
  

  1. (1 四川农业大学农学院, 雅安625014 ; 2 四川农业大学林学园艺学院, 雅安625014)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2001-04-25 发布日期:2001-04-25

Changes of Organic Acid Synthetase Activity during Fruit Development of Navel Orange (Citrus sinesis Osbeck)  

Wen Tao1, Xiong Qing’e1, Zeng Weiguang2, and Liu Yuanpeng2
  

  1. (1Agriculture College , Sichuan Agricultural University , Ya’an 625014;2 Forestry Horticulture College , Sichuan Agricultural University , Ya’an 625014)
  • Received:1900-01-01 Revised:1900-01-01 Online:2001-04-25 Published:2001-04-25

摘要: 以红桔砧10 年生罗伯逊脐橙为试材, 研究了果实发育过程中有机酸代谢相关酶活性的变化及其与有机酸积累的关系。结果表明: 在整个果实发育过程中, 柠檬酸合成酶(CS) 和磷酸烯醇式丙酮酸羧化酶(PEPC) 活性与有机酸含量呈极显著正相关。这不仅证明脐橙果实中存在通过CO2 固定合成有机酸的途径, 还说明CS、PEPC 是有机酸合成的关键酶, 抑制CS 的活性能降低果实中有机酸的生成量。

关键词: 脐橙, 果实, 有机酸, 柠檬酸合成酶, 磷酸烯醇式丙酮酸羧化酶

Abstract: Using Robertson Navel (madarin rootstock ten years) as a testing material , the activity variance of enzyme related to organic acid metabolism and its relationship with the organic acid accumulation were studied. The result shows : There is a significantly positive correlation between the activity of Citrate synthase ( CS) or Phosphoenolpyruvate carboxylase (PEPC) and organic acid , Their correlation coefficient ( r) are equal to 0. 916 0 and
0. 877 9 respectively. This not only proves that CO2 fixation is a main pathway of organic acid synthesis in Navel orange fruit ; but also proves that CS and PEPC are the key enzymes of organic acid synthesis , and efficiently inhibiting CS and PEPC activity could reduce the organic acid content of orange fruit.

Key words: Navel orange (Citrus sinesis Osbeck), Fruit, Organic acid, Citrate synthase (CS), Phosphoenolpyruvate carboxylase (PEPC)

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