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园艺学报 ›› 2015, Vol. 42 ›› Issue (10): 1974-1982.doi: 10.16420/j.issn.0513-353x.2015-0213

• 蔬菜 • 上一篇    下一篇

不同施氮水平下秋马铃薯的生长以及StNR和StAT的表达

胡新喜1,冯艳青1,雷 艳1,刘明月1,熊兴耀1,2,*   

  1. 1湖南农业大学园艺园林学院,湖南省马铃薯工程技术研究中心,长沙 410128;2中国农业科学院蔬菜花卉研究所,北京 100081
  • 出版日期:2015-10-25 发布日期:2015-10-25
  • 基金资助:

    公益性行业科研专项经费项目(201203096);国家现代农业产业技术体系建设专项资金项目(CARS-10-P19)

Effects of Nitrogen Rate on Plant Growth of Autumn Potato Crop and Gene Expression of Nitrate Reductase and Ammonium Transporter

HU Xin-xi1,FENG Yan-qing1,LEI Yan1,LIU Ming-yue1,and XIONG Xing-yao1,2,*   

  1. 1Hunan Provincial Engineering Research Center for Potatoes,College of Horticulture and Landscape,Hunan Agricultural University,Changsha 410128,China;2The Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China
  • Online:2015-10-25 Published:2015-10-25

摘要:

通过对不同氮素水平下秋马铃薯生长及叶片氮素转运与吸收相关基因表达的研究,结果表明,块茎形成期和块茎膨大期叶片叶绿素含量(SPAD值)随施氮量的增加而增加;硝酸还原酶基因(StNR)和铵转运蛋白基因(StAT)的表达量随施氮量的增加而降低,StNR和StAT的表达量与施氮量、叶绿素含量呈极显著负相关(P < 0.01)。随着施氮量的增加,马铃薯株高和茎粗呈上升趋势,但单株结薯数、单薯质量和产量均呈先上升后下降的趋势,块茎淀粉含量呈降低趋势;秋马铃薯的施氮水平在90 ~ 135 kg · hm-2为宜。土壤和块茎硝态氮含量均随着施氮量的增加而增加,0 ~ 20 cm深土壤硝态氮含量在1.55 ~ 9.34 mg · kg-1;块茎硝态氮含量在20.58 ~ 49.81 mg · kg-1,各处理间差异极显著(P < 0.01)。研究结果可为大田马铃薯植株氮素营养分子诊断技术的建立及大田氮素运筹提供理论依据。

关键词: 马铃薯, 硝酸还原酶, 铵转运蛋白, 施氮水平, 光合作用

Abstract:

Effects of nitrogen(N)rate on the growth of autumn field-grown potato plants and the expression of nitrate reductase gene(StNR)and ammonium transporter gene(StAT)were studied. SPAD reading increased as fertilizer N rate increased from 0 to 360 kg · hm-2 at the tuber initiation and the tuber bulking periods;Interestingly,the expression of StNR and StAT in leaf tissue decreased as fertilizer N rate increased,exhibiting a significant(P < 0.01)negative correlation with nitrate application rate and SPAD reading at the tuber initiation and the tuber bulking periods. Plant height and stem diameter increased with increasing fertilizer N rate. For the total tuber number per plant, the mean tuber weight and the total yield,they increased to the maximum values,then decreased while fertilizer N rate increased. Forstarch content,it decreased with increasing fertilizer N rate. Taken together,fertilizer N rate at 90–135 kg · hm-2 can be recommended for the autumn potato crop. Additional analysis demonstrated that as fertilizer N application increased,soil and tuber ammonium-N concentration increased. In soil of 0–20 cm depth in the potato root zone,ammonium-N level was between 1.55 to 9.34 mg · kg-1;And no significant increase was observed when N fertilizer N rate was below 360 kg · hm-2. In tubers,ammonium-N concentration was between 20.58 to 49.81 mg · kg-1;And as fertilizer N rate increased,a significant increase(P < 0.01)of tuber ammonium-N was observed. The results would provide theoretical basis for establishment of the molecular detection method for nitrogen sufficiency in field-grown potato plants and field nitrogen fertilizer managements.

Key words: Solanum tuberosum, nitrate reductase, ammonium transporter, nitrogen rate, photosynthesis

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