https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2013, Vol. 40 ›› Issue (5): 896-.

• 蔬菜 • 上一篇    下一篇

黄瓜果实弯曲相关基因Cs14-3-3 的克隆及表达分析

徐 圆, 秦智伟, 周秀艳   

  1. 东北农业大学园艺学院,农业部东北地区园艺作物生物学与种质创制重点实验室,黑龙江省寒地蔬菜生物学重点
    实验室,哈尔滨150030
  • 出版日期:2013-05-25 发布日期:2013-05-25

Cloning and Expression Analysis of Fruit Bending Related Gene Cs14-3-3
in Cucumber

XU   Yuan, QIN  Zhi-Wei, ZHOU  Xiu-Yan   

  • Online:2013-05-25 Published:2013-05-25

摘要: 以黄瓜(Cucumis sativus L.)果实易发生弯曲的品种‘长春密刺’为试验材料,采用RT-PCR
技术从果皮中分离并克隆了14-3-3 蛋白基因,并将此基因命名为Cs14-3-3Cs14-3-3 基因cDNA 全
长792 bp,编码263 个氨基酸,分子量29 589.287 Da,等电点为4.585。生物信息学分析表明此基因含有
14-3-3 蛋白基因的典型结构域,与其他物种14-3-3 蛋白基因核苷酸序列同源性达到75%以上,编码的氨
基酸序列同源性达到85%以上,属于14-3-3 蛋白基因家族。同时,采用实时荧光定量PCR 方法对顺直果
实和弯曲果实腹部及脊部在果实开花的2、4、6、8、10、12 d 的基因表达量进行了研究。结果显示,在
果实发育各个时期,Cs14-3-3 的表达量均为在弯曲果实腹部 > 顺直果实 > 弯曲果实脊部的表达量;在
各个部位,Cs14-3-3 在果实开花2 d 的表达量明显高于开花后其他时期。试验结果表明,Cs14-3-3 基因为
黄瓜果实弯曲相关基因,在黄瓜果实开花早期发育过程中起重要作用。

关键词: 黄瓜, 果实弯曲, Cs14-3-3 基因, 表达分析

Abstract: Abstract:By RT-PCR technology,we separated and cloned the 14-3-3 protein gene from the peel of
the cucumber(Cucumis sativus L.)bending variety‘Changchunmici’,named Cs14-3-3.The full length
cDNA of Cs14-3-3 is 792 bp,encoding a protein of 263 amino acids.Its molecular mass is 29 589.287 Da,
and pI is 4.585. Bioinformatic analysis showed that the protein of Cs14-3-3 possesses the basic structure of
14-3-3 proteins,and shares high homology with the known 14-3-3 proteins,which is belonging to the
14-3-3 protein family. At the same time,by using real-time fluorescence quantitative PCR(RT-qPCR),
we studied the expression of mRNA in straight fruit and the abdomen and the ridge of the bending fruit in
the periods of 2、4、6、8、10、12 days after flowering. The result showed that in each period,the expression
level of Cs14-3-3 from high to low order is bending fruit abdomen,straight fruit,bending fruit ridge,and
in each part,the expression level of Cs14-3-3 in 2 days after flowering obviously higher than that in the
other periods. In conclusion,it was preliminarily revealed that Cs14-3-3 gene is related to fruit bending in
cucumber,and plays an important role in the period of early fruit development after flowering.

Key words: cucumber, fruit bending, Cs14-3-3 gene, expression analysis

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