园艺学报 ›› 2012, Vol. 39 ›› Issue (7): 1313-.doi:

• 蔬菜 • 上一篇    下一篇

芥菜Fosmid 文库构建及B 基因组细胞学标记的筛选利用

 彭元凤, 孟德璇, 黄玉碧, 王桂香, 刘 凡   

  1. 四川农业大学玉米研究所,四川温江 611130; 北京市农林科学院蔬菜研究中心,北京 100097;中国农业大学国家玉米改良中心,北京 100193
  • 出版日期:2012-07-25 发布日期:2012-07-25

Construction of Genomic Fosmid Library of Brassica juncea and Screening of Cytological Markers for B-genome Chromosomes

PENG  Yuan-Feng, MENG  De-Xuan, HUANG  Yu-Bi, WANG  Gui-Xiang, LIU   Fan   

  1. Maize Research Institute of Sichuan Agricultural University,Wenjiang,Sichuan 611130,China;Beijing VegetableResearch Center,Beijing 100097,China;China National Maize Improvement Center of China Agricultural University,Beijing 100193,Chinan
  • Online:2012-07-25 Published:2012-07-25

摘要: 构建了由60 000 个克隆组成的芥菜无偏倚Fosmid 文库,该文库外源片段插入率为100%,外源DNA 平均插入长度为32 kb,文库覆盖率约为芥菜基因组的1.8 倍。利用不同来源的分子标记筛选文库,得到的阳性单克隆经荧光原位杂交(FISH)鉴定后,获得两类B 基因组细胞学标记,一类在所有染色体上都有信号,另一类仅在一对染色体上有信号。

关键词: 芥菜, Fosmid 文库, 细胞学标记, 分子标记, FISH

Abstract: In this study,an unbiased Fosmid library of Brassica juncea was established,which consisted of 60 000 clones with 100% inserting frequency. In the constructed library,the size of average inserts was approximately 32 kb,corresponding to 1.8 genome equivalents. Subsequently,two types of
B-genome cytological markers were identified by means of library screening and chromosome fluorescence in situ hybridization(FISH). One type was that the signal located on all chromosomes,and the other one was that the signal was detected only on one pair of chromosomes.

Key words: Brassica juncea, fosmid library, cytological marker, molecular marker, FISH