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园艺学报 ›› 2020, Vol. 47 ›› Issue (2): 381-389.doi: 10.16420/j.issn.0513-353x.2019-0769

• 研究报告 • 上一篇    下一篇

过表达 SbRFP1 基因提高马铃薯组培苗的耐盐性

赵喜娟 1,2,张会灵 1,*,侯 娟 2,3,张菊平 1,陈苏丹 1,宋波涛 2   

  1. 1 河南科技大学林学院,河南洛阳 471000;2 华中农业大学园艺林学学院,武汉 430070;3 河南农业大学园艺学院,郑州 450002
  • 出版日期:2020-02-25 发布日期:2020-02-25
  • 基金资助:
    国家自然科学基金项目(31401437);河南省高等学校重点科研项目(20A210009);河南省科技攻关项目(182102110401);河南科技大学青年学术带头人培育基金项目(4026-13490004)

Overexpression of SbRFP1 Gene Improves Salt Tolerance of in Vitro Potato Plantlets

ZHAO Xijuan1,2,ZHANG Huiling1,*,HOU Juan2,3,ZHANG Juping1,CHEN Sudan1,and SONG Botao2   

  1. 1College of Forestry,Henan University of Science and Technology,Luoyang,Henan 471000,China;2 College of Horticulture and Forestry Sciences,Huazhong Agricultural University,Wuhan 430070,China;3 College of Horticulture,Henan Agricultural University,Zhengzhou 450002,China
  • Online:2020-02-25 Published:2020-02-25

摘要: 利用不同浓度 NaCl 处理过表达 SbRFP1 的转基因株系和对照组培苗,结果显示 5 g · L-1 和8 g · L-1 的 NaCl 胁迫时,转基因株系的株高、根长和鲜质量均显著超过非转基因对照。转录组测序发现F-box 基因(PGSC0003DMG400013116)和 GDSL(Gly-Asp-Ser-Leu)酯酶基因(PGSC0003DMG400007815)等在转基因株系中的表达量显著高于非转基因对照,且经 NaCl 诱导上调表达。用外源物质处理非转基因组培苗并检测差异基因的表达量,结果显示差异表达基因受水杨酸(SA)等多种激素和 NaCl 诱导表达。说明 SbRFP1 可能是通过调控 F-box 和 GDSL 酯酶等胁迫相关基因的表达提高马铃薯组培苗的耐盐性。

关键词: 马铃薯, SbRFP1, NaCl 胁迫, 表达分析

Abstract: The in vitro plantlets of transgenic lines overexpressed SbRFP1 and control were treated with NaCl at different concentrations. Results showed that the plant height,root length and fresh weight of transgenic lines were significantly higher than those of control under 5 g · L-1 or 8 g · L-1 NaCl stress. Transcriptome sequencing of transgenic lines and non-transgenic control tubers displayed that the expression levels of F-box gene(PGSC0003DMG400013116)and GDSL(Gly-Asp-Ser-Leu)gene(PGSC0003DMG400007815)in transgenic lines were significantly higher than those in control,as well as up-regulated by NaCl. The in vitro plantlets of E3(non-transgenic)were treated with exogenous substances and the expression of differentially expressed genes were detected. Results showed that the differentially expressed genes were induced by SA and other hormones,as well as NaCl. It is suggested that SbRFP1 may improve the salt tolerance of potato by regulating the expression of stress-related genes such as F-box gene and GDSL gene.

Key words: potato, SbRFP1, NaCl stress, expression analysis

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