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园艺学报 ›› 2020, Vol. 47 ›› Issue (2): 345-354.doi: 10.16420/j.issn.0513-353x.2019-0512

• 研究报告 • 上一篇    下一篇

无核酸橙资源‘抛橘’的遗传鉴定

韦壮敏 1,*,李蒙蒙 1,*,杨晓明 2,袁晓慧 3,叶俊丽 1,谢宗周 1,邓秀新 1,柴利军 1,**   

  1. 1 华中农业大学园艺植物生物学教育部重点实验室,武汉 430070;2 南京林业大学,南方现代林业协同创新中心,南京 210037;3 龙陵恒冠泰达农业发展有限公司,云南龙陵 678300
  • 出版日期:2020-02-25 发布日期:2020-02-25
  • 基金资助:
    国家现代农业产业技术体系建设专项资金项目(CARS-27);云南省科技厅科技入滇专项(2018IB013);云南省科技厅院士工作站(2017IC035)

Genetic Background Analysis of Seedless Sour Orange‘Paoju’

WEI Zhuangmin1,*,LI Mengmeng1,*,YANG Xiaoming2,YUAN Xiaohui3,YE Junli1,XIE Zongzhou1, DENG Xiuxin1,and CHAI Lijun1,**   

  1. 1Key Laboratory of Horticultural Plant Biology(Ministry of Education),Huazhong Agricultural University,Wuhan 430070,China;2Co-Innovation Center for the Sustainable Forestry in Southern China,Nanjing Forestry University,Nanjing 210037,China;3Longling Hengguan Taida Agricultural Development Co. LTD,Longling,Yunnan 678300,China
  • Online:2020-02-25 Published:2020-02-25

摘要: 以福建无核酸橙资源‘抛橘’为研究对象,运用 cpSSR(叶绿体 SSR)标记鉴定其母系亲本,nSSR(核 SSR)标记鉴定其父系亲本,并构建 UPGMA 聚类树。7 对 cpSSR 引物扩增出 22 个条带,平均每个位点 3.14 个条带,平均期望杂合度(He)和 Shannon 信息指数(I)分别为 0.47 和 0.81。13 对nSSR 引物扩增出 65 个条带,平均每个位点 5 个条带,He 和 I 分别为 0.66 和 1.27。根据 UPGMA 聚类分析结果推测‘抛橘’是以柚类(Citrus maxima)× 酸橙类(Citrus aurantium)杂交而来,并与代代酸橙有密切联系。以甜橙为参考基因组对‘抛橘’进行基因组重测序的分析结果显示:‘抛橘’与酸橙的基因组序列相似度在 85%以上,进一步证明‘抛橘’可能是酸橙的一种类型。

关键词: 柑橘, 遗传鉴定, SSR, 重测序

Abstract: The objective of this study was to analyze the genetic background of a seedless sour orange species called‘Paoju’that is cultivated in Fujian Province using cpSSR, nSSR and genomic resequencing. We found that seven pairs of cpSSR primers could amplify 22 bands with an average of 3.14 bands per locus. The average expected heterozygosity(He)and Shannon information index(I)were 0.47 and 0.81, respectively. Thirteen pairs of nSSR primers amplified 65 bands with an average of 5 bands per locus. The average He and I were 0.66 and 1.27,respectively. A UPGMA analysis that used the data from the cpSSR and nSSR experiments demonstrated that‘Paoju’is a hybrid derived from a Citrus maxima × C. aurantium and that‘Paoju’is closely related to Daidai Sour Orange. A resequencing analysis with sweet orange as reference genome showed that the similarity rate between‘Paoju’and sour orange was more than 85%. Based on these data,we speculate that‘Paoju’may be a type of sour orange.

Key words: citrus, genetic identification, SSR, resequencing

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