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园艺学报 ›› 2019, Vol. 46 ›› Issue (6): 1079-1092.doi: 10.16420/j.issn.0513-353x.2018-1040

• 研究论文 • 上一篇    下一篇

基于叶球转录组数据比较的甘蓝杂种优势分析

李升娟,许忠民*,郭 佳,张恩慧,姜 娇,石汶汶   

  1. 西北农林科技大学园艺学院,陕西杨凌 712100
  • 出版日期:2019-06-25 发布日期:2019-06-25
  • 基金资助:
    国家重点研发计划项目(2016YFD0101702);国家现代农业产业技术体系建设专项资金项目(CARS-25);陕西省重点研发计划项目(2018NY-059);西安市科技计划项目(201806113YF01NC09)

Comparative Heterosis Analysis of Cabbage Based on Transcriptome Data of Cabbage Heads Leaves

LI Shengjuan,XU Zhongmin*,GUO Jia,ZHANG Enhui,JIANG Jiao,and SHI Wenwen   

  1. College of Horticulture,Northwest A & F University,Yangling,Shaanxi 712100,China
  • Online:2019-06-25 Published:2019-06-25

摘要: 对甘蓝两个杂交组合F1的杂种优势进行了田间性状统计分析以及叶球转录组测序分析。所调查的9个园艺学性状中,单球质量和球主叶柄质量在F1代及其亲本之间差异显著,其中单球质量的中亲优势和超亲优势在两个组合F1中表现突出,即产量杂种优势较为明显。通过叶球转录组分析,分别筛选获得了4组差异表达基因,在相同标准下,两个杂交组合中父本与F1代的差异表达基因明显多于母本与F1代的差异表达基因,表明母本的表达谱与F1更相似,即母本在F1叶球杂种优势形成中的贡献较大,而且上调差异表达基因的差异倍数明显高于下调差异表达基因,表明上调基因在F1代甘蓝叶球杂种优势建成中有重要作用。进一步将差异表达基因进行GO(Gene ontology)分类、COG(Cluster of orthologous groups of proteins)分类、KEGG(Kyoto encyclopedia of genes and genomes)通路富集分析以及可变剪接分析,发现差异表达基因显著富集到生长发育、碳水化合物转运和代谢、信号转导以及氨基酸的合成、转运和代谢等途径。随机选取7个差异表达基因进行实时荧光定量PCR验证,结果与RNA-seq数据基本一致,证明转录组数据的可靠性。本研究中获得的与甘蓝叶球杂种优势形成相关的差异表达基因,为后续甘蓝杂种优势分子机制研究提供数据支持。

关键词: 甘蓝, 杂种优势, 转录组分析, 差异表达基因

Abstract: In two F1 hybrids,we performed heterosis analysis through field traits survey and transcriptome sequencing of cabbage heads leaves. The result found that among the nine horticultural traits,head weight and main petiole weight showed significant differences between F1 hybrids and their parents,among which the head weight exhibited a high value of MPH(mid-parent heterosis)and HPH(high-parent heterosis)in the two F1 hybrids,which means the yield heterosis is obvious. Four groups of differentially expressed genes(DEGs)were compared through transcriptome analysis of cabbage heads leaves. We found that the number of DEGs between F1 hybrids and paternal lines were significantly higher than that between F1 hybrids and their maternal lines in the same criteria. It means similar gene expression profile between maternal lines and their F1 hybrids or a bigger contribution of maternal lines than paternal lines in the formation process of cabbage heads leaves of F1 hybrids. Besides,the fold change of up-regulated genes were higher than that of down-regulated genes,which implied an important role of up-regulated genes in the formation process of cabbage heads leaves of F1 hybrids. The DEGs were further conducted GO(Gene Ontology),COG(Cluster of Orthologous Groups of proteins),KEGG(Kyoto Encyclopedia of Genes and Genomes)pathway enrichment and alternative splicing analysis. The result found that DEGs are highly involved in growth and development,carbohydrate transport and metabolism,signal transduction and amino acid biosynthesis,transport and metabolism pathways. Seven DEGs were randomly selected to perform qRT-PCR and the result was basically consistent with RNA-seq,demonstrating the reliability of transcriptome data. The DEGs that we screened associated with the formation process of heterosis of cabbage heads leaves,able to provide data support for the subsequent research on the molecular mechanism of cabbage heterosis.

Key words: Brassica oleracea L. var. capitata, heterosis, transcriptome analysis, differentially expressed genes(DEGs)

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