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园艺学报 ›› 2019, Vol. 46 ›› Issue (6): 1093-1111.doi: 10.16420/j.issn.0513-353x.2018-0873

• 研究论文 • 上一篇    下一篇

黄瓜SAUR基因家族的鉴定与表达分析

王红飞,尚庆茂*   

  1. 中国农业科学院蔬菜花卉研究所,农业部园艺作物生物学与种质创制重点实验室,北京 100081
  • 出版日期:2019-06-25 发布日期:2019-06-25
  • 基金资助:
    国家自然科学基金项目(31172001);国家现代农业产业技术体系建设专项资金项目(CARS-25);公益性行业(农业)科研专项(201303014);中国农业科学院科技创新工程项目(CAAS-ASTIP IVFCAAS)

Genome-wide Identification and Expression Analysis of the SAUR Gene Family in Cucumis sativus

WANG Hongfei and SHANG Qingmao*   

  1. Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Key Laboratory of Biology and Genetic Improvement of Horticultural Crops,Ministry of Agriculture,Beijing 100081,China
  • Online:2019-06-25 Published:2019-06-25

摘要: SAUR(Small auxin-up RNA)是生长素早期响应基因。基于黄瓜(Cucumis sativus L.)基因组数据,对CsaSAUR进行鉴定以及染色体定位、基因结构、启动子顺式作用元件分析,并通过qRT-PCR技术检测基因组织表达模式和对弱光、高温及高水势的响应。结果表明,在黄瓜基因组中共鉴定到73个SAUR,这些基因非随机地分布在7条染色体上,60%成簇分布;系统进化分析显示,CsaSAURs可分为8个进化群,各群基因数量不等;进一步分析家族成员的启动子序列,鉴定到IAA、ABA、GA、JA、SA及干旱、低温和机械损伤等信号响应的顺式作用元件;该家族成员具有不同的表达模式,弱光、高温和高水势可激活或抑制部分家族成员的表达,在下胚轴特异表达的18个CsaSAUR基因中,仅CsaSAUR15同时受弱光、高温、高水势3种信号诱导,表达上调。

关键词: 黄瓜, SAUR, 基因表达, 进化分析

Abstract: SAURs(Small auxin-up RNAs)are the early auxin-responsive genes,which play critical roles in regulating plant growth and development. In the present study,we identified the members of SAUR gene family in cucumber(Cucumis sativus)and analyzed their evolution,gene structure,tissue-specific transcription patterns,and responses to light intensity,temperature,and water potential. A total of 73 CasSAUR genes were identified and they were distributed onto the 7 chromosomes with 60% genes appearing in 2 clusters. The phylogenetic tree indicated that CsaSAURs could be further divided into 8 subfamilies and each of subfamily included a range of genes. The cis-acting elements,responding to IAA,ABA,GA,JA,SA,drought,low temperature,and mechanical damage,were identified by analyzing the promoter sequence of CsaSAURs. The members of CsaSAUR gene family exhibited different expression patterns and some of them can be activated or repressed by low light,high temperature,and high water potential. Among the 18 CsaSAURs highly expressed in hypocotyl,only CsaSAUR15 is up-regulated by the three environmental factors:low light,high temperature and high water potential.

Key words: cucumber, SAUR, gene expression, phylogeny analysis

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