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园艺学报 ›› 2019, Vol. 46 ›› Issue (7): 1359-1372.doi: 10.16420/j.issn.0513-353x.2018-0753

• 研究报告 • 上一篇    下一篇

龙眼EST-SSR标记开发及无患子科5个属种质遗传多样性分析

胡文舜,陈秀萍,郑少泉*   

  1. 福建省农业科学院果树研究所,福建省龙眼枇杷育种工程技术研究中心,福州 350013
  • 出版日期:2019-07-25 发布日期:2019-07-25
  • 基金资助:
    国家自然科学基金项目(31701884);省属公益类科研院所基本科研专项(2018R1013-5);国家现代农业产业技术体系建设专项资金项目(CARS-32-06);福建省农业科学院科技创新团队项目(STIT2017-1-4);农业农村部物种品种资源保护(热带作物)项目(151821301354052701);国家万人计划——百千万领军人才项目(2016-)

EST-SSR Markers Developed from Dimocarpus longan and Their Application in Genetic Diversity Analysis of Five Genera of Sapindaceae

HU Wenshun,CHEN Xiuping,and ZHENG Shaoquan*   

  1. Fruit Research Institute,Fujian Academy of Agricultrual Sciences,Fujian Breeding Engineering Technology Research Center for Longan & Loquat,Fuzhou 350013,China
  • Online:2019-07-25 Published:2019-07-25

摘要: 基于龙眼品种‘香脆’果实的转录组测序数据库,开发龙眼EST-SSR引物,评价其在无患子、红毛丹、荔枝、龙荔等4个近缘属植物上的通用性,并进行无患子科5个属55份种质的遗传多样性分析。在13 934条龙眼Unigene中搜索到SSR位点6 683个,SSR发生频率为47.96%,平均分布距离为4.48 kb;单、二和三核苷酸重复是主要的SSR类型,分别占52.78%、26.25%和20.19%;设计出4 670对SSR引物,随机合成185对不同重复类型的SSR引物进行验证,有效扩增率为78.92%(146对),其中多态性引物占34.25%(50对),条带多态率50% ~ 100%;龙眼多态性引物在无患子属、韶子属、荔枝属、龙荔属等4个近缘属上的转移率分别为38.30%、38.30%、68.09%和74.47%。UPGMA聚类分析显示,55份材料在遗传相似系数0.561处明显地划分为龙眼、龙荔、荔枝、无患子和红毛丹5个大类。

关键词: 无患子科, 龙眼, SSR, 转录组

Abstract: Based on the transcriptome sequencing data of flesh of a new longan cultivar‘Xiangcui’,this study developed the novel EST-derived SSR markers to access their applicability on the 4 relative plants (Sapindus mukorossi,Rambutan,Litchi chinensis and Dimocarpus confinis)and then explored the genetic relationship among the 55 germplasms of 5 genera of Sapindaceae family. The results showed that a total of 6 683 SSR loci were mined from 13 934 unigenes with a frequence of 47.96% and mean distance of 4.48 kb. The most abundant type of repeat motif was single nucleotide(52.78%),followed by dinucleotide(26.25%)and trinucleotide(20.19%). 4 670 pairs of SSR primers were designed and 185 pairs of primers with different repeat motifs were randomly selected for further verification. Of all,the effective PCR success rate shared 78.92%(146 pairs),polymorphism rate shared 34.25%(50 pairs),polymorphic band rate ranged from 50% to 100%. And the rate of longan polymorphic primers transferring to S. mukorossi Gaertn.,N. lappaceum Linn.,L. chinensis Sonn. and D. confinis H.S.Lo were 38.30%,38.30%,68.09% and 74.47%,respectively. Furthermore,with the genetic similarity coefficient of 0.561,UPGMA clustering analysis divided the 55 germplasms into 5 groups,including longan,longli,litchi,Sapindus,and Nephelium.

Key words: Sapindaceae, Dimocarpus longan, SSR, transcriptome

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