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ACTA HORTICULTURAE SINICA ›› 2008, Vol. 35 ›› Issue (12): 1773-1780.

• 蔬菜 • Previous Articles     Next Articles

Genetic analysis and Specific Fragments Linked to Powdery Mildew Resistant Gene Pm-2F in Melon

Zhang Hai-ying1,Su Fang1,Guo Shao-gui1,Gong Guo-yi1,Yi Hong-ping2,Wu Ming-zhu2,and Xu Yong1*   

  1. (1National Engineering Research Center for Vegetables, Beijing 100097, China; 2Hamigua Research Center, Xinjiang Academy of Agricultural Sciences, Urumqi 830000, China)
  • Received:2008-07-08 Revised:2008-11-06 Online:2008-12-25 Published:2008-12-25
  • Contact: Xu Yong

Abstract:

Podosphaera xanthii (DC.)VP Gelyuta race 2F caused melon (Cucumis melo L.) powdery mildew was the prevailing race in Beijing. The segregation of resistance had been evaluated in F2S6 population of the cross between the resistant Japanese cantaloupe(Cucumis melo L. ssp. melo.convar. cantalupa (Pang.) Greb.)line K7-1 and the susceptible Xinjiang Hami melon(Cucumis melo L. ssp. melo. convar. ameri (Pang.) Greb.)line K7-2. The resistance to P. xanthii race 2F in K7-1 was controlled by one single dominant Pm-2F gene. SSR technique had been used for the identification of markers linked to powdery mildew resistance. Two specific fragments CMBR120-172 and CMBR8-98 were closely linked to Pm-2F gene at a distance of 1 cM and 3 cM, respectively. The efficiency of CMBR120-172 was about 87.5% in 120 melon germplasms. The markers identified in this experminent were closest to Pm-2F gene to date, and the research, therefore, can be used in melon molecular breeding.

Key words: melon, Cucumis melo L., powdery mildew, resistant gene, specific fragments

CLC Number: