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ACTA HORTICULTURAE SINICA ›› 2019, Vol. 46 ›› Issue (12): 2347-2358.doi: 10.16420/j.issn.0513-353x.2019-0118

• Research Papers • Previous Articles     Next Articles

Construction of Melon Genetic Linkage Map and QTL Analysis of Fruit Related Traits in Snake Melon × Wild Type Melon Genetic Background

WANG Chenhui,LUAN Feishi*,GAO Peng*,LIU Shi,and XIE Zhiqiang   

  1. College of Horticulture and Landscape Architecture,Northeast Agricultural University;Ministry of Agriculture Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in Northeast China,Harbin 150030,China
  • Online:2019-12-25 Published:2019-12-25

Abstract: Based on the importance on the fruits related traits on melon production,QTLs locus linked with the fruits related traits in melon was analyzed in this paper. F2 populations obtained from Snake melon(Cucumis melo L.)‘M4-75’and wild type melon(Cucumis melo L.)‘X207’as parents were used as materials. Cleaved amplified polymorphic sequence(CAPS)markers were developed to construct a genetic linkage map and detect the QTLs(quantitative trait locus)related with fruit traits in melon based on the parental genome re-sequencing data. As the results,totally 180 CAPS markers were screened which showed polymorphism between parents and for the genetic linkage map construction. Finally,the map contained 165 markers and grouped into 12 linkage groups corresponded to the 12 chromosomes in melon,spanning a total length of 1 019.81 cM,with an average of 6.18 cM between markers. Meanwhile,fruits related traits(fruit shape,flesh thickness and flesh pH)in melon were all quantitative traits. Totally 14 QTLs associated with the traits were detected which distributed among Chromosome 1,2,3,4,5,6,7,10,11. The LOD(log of odds)score ranged from 2.67 to 9.63,which could explain the phenotypic variation rate of 7.60%–35.18%. Nine QTLs related with fruit shape,3 QTLs for flesh thickness and 2 QTLs for flesh pH.

Key words: melon, fruit length, CAPS marker, genetic linkage map, QTL

CLC Number: