A Highly Efficient System Establishment of Transient Expression in Lettuce

ACTA HORTICULTURAE SINICA ›› 2006, Vol. 33 ›› Issue (2): 405-407.

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A Highly Efficient System Establishment of Transient Expression in Lettuce

Li Jing¹;Chen Min^2*;Liu Xianwei²; Shen Fafu¹;Wang Peng^2*

(¹The Key Laboratory of Crop Biology of Shandong, School of Agronomy, Shandong Agricultural University, Tai'an 271018,China; 2 The State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, Ji'nan 250100, China)

Received:2005-07-27 Revised:2005-12-09 Online:2006-04-25 Published:2006-04-25

Abstract

Abstract: Using Agrobacterium vacuum infiltration method the plant bivalent expression vector pBI121 containing GUS as a report gene was transformed into lettuce (Lactuca sativa L. sp). Through orthogonal experiment, a highly efficient system of transient expression was observed in plant intact leaves when they were
treated with 200 μmol/L acetosyringone, 0.8 OD₆₀₀ bacterium density, vacuum 30 min and co-culture 6 days. Transgenic expression level of the optimal condition is improved 16.3 times than that of the control.

Key words: Lactuca sativa, Agrobacteriumtum efaciens, Vacuum infiltration, GUS, Transient expression

Li Jing;Chen Min;Liu Xianwei;Shen Fafu;Wang Peng. A Highly Efficient System Establishment of Transient Expression in Lettuce[J]. ACTA HORTICULTURAE SINICA, 2006, 33(2): 405-407.

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A Highly Efficient System Establishment of Transient Expression in Lettuce

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