Studies on the Proteoglycans Isolated from the Wild Lactarius deliciosus and Lactarius hatsudak

ACTA HORTICULTURAE SINICA ›› 2006, Vol. 33 ›› Issue (2): 408-410.

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Studies on the Proteoglycans Isolated from the Wild Lactarius deliciosus and Lactarius hatsudak

Dong Aiwen^{1, 2};Fu Xinghui¹; Huang Mei'e¹;Chen Yang¹

(¹College of Urban and Rural Resources and Planning Sciences, Jishou University, Zhangjiajie 427000, China; ²College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China)

Received:2005-06-30 Revised:2005-12-14 Online:2006-04-25 Published:2006-04-25

Abstract

Abstract:
Proteoglycan was extracted from the Lactaricus deliciosus Gray and Lactarius hatsudak Tanake by app rop riate solvent, method and condition. Proteoglycan was further isolated and purified by Sevage, DEAE-cellulose columns and SephdexG-100. After purified proteoglycans was analyzed by IR, UV-spectrum,
the contents of protein and sugar were measured. The result shows that the protein and sugar content in the proteoglycan of Lactarius deliciosus were 24.53% and 68.83% , and that in proteoglycan of Lactarius hatsudak were 15.53% and 66.52%. At the same time two p roteoglycans have antioxidation. TLC analysis of proteoglycan hydrolate showed that those sugars of Lactarius deliciosus were mainly composed of glucose, fructose and mannose while sugars of Lactarius hatsudak were mainly composed of glucose, fructose and xylose.

Key words: Lactarius deliciosus, Lactarius hatsudak, Proteoglycan, Extraction, Isolate, Purify

Dong Aiwen;;Fu Xinghui;Huang Mei'e;Chen Yang. Studies on the Proteoglycans Isolated from the Wild Lactarius deliciosus and Lactarius hatsudak[J]. ACTA HORTICULTURAE SINICA, 2006, 33(2): 408-410.

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Studies on the Proteoglycans Isolated from the Wild Lactarius deliciosus and Lactarius hatsudak

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