Abstract: OPR is a key enzyme in jasmonic acid biosynthesis pathway，a full-length cDNA named
GhOPR3 was cloned in Gladiolus hybridus‘Rose Supreme’corms by RT-PCR and RACE. The results of
quantitative RT-PCR showed that GhOPR3 gene was expressed in leaf，flower，root，stolon，corm and
cormel，and the relatively high expression level of GhOPR3 was observed in cormel and stolon.
Meanwhile，the expression level and the endogenous MJ content in corms steadily increased under MJ
treatment with a raising concentration gradients from 0.1 mmol · L-1 to 0.5 mmol · L-1. GhOPR3 was
overexpressed in Arabidopsis，which increased the salt tolerance and drought-resistant in Arabidopsis.
After mechanical damage，the expression level of related resistance genes and the content of endogenous
MJ were increased.

Key words: Gladiolus hybridus, JA, OPR, heterologous expression, stress resistance