Forecasting Analysis of the Grape Flowering Pathway Based on the Grape EST Database

ACTA HORTICULTURAE SINICA ›› 2014, Vol. 41 ›› Issue (1): 26-34.

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Forecasting Analysis of the Grape Flowering Pathway Based on the Grape EST Database

LIU Dan1,*，MU Qian1,*，LI Xiao-peng1，LIU Geng-sen2，LI Yu1，ZHANG Yan-ping1，and FANG Jing-gui1,**

1College of Horticulture，Nanjing Agricultural University，Nanjing 210095，China；2College of Landscape and Horticulture，Qingdao Agricultural University，Qingdao，Shandong 266109，China

Online:2014-01-25 Published:2014-01-25

Abstract

Abstract: Homologous grape EST sequences were searched against the grape EST database using the
corresponding flowering pathway gene sequences from Arabidopsis as the probes. The existence and
expression of related genes involved in the flowering pathway in grape were further studied by
half-qRT-PCR using cDNA，prepared from different grape organs at various developing stages，as PCR
template，bashed on which，the grape flowering pathways were thoroughly forecasted. The study results
also indicated that the expression levels of the grape flowering pathway genes showed some
spatiotemporal differences.

Key words: grape, EST, flowering pathway, genes

CLC Number:

S 663.1

LIU Dan, MU Qian, LI Xiao-Peng, LIU Geng-Sen, LI Yu, ZHANG Yan-苹, FANG Jing-Gui. Forecasting Analysis of the Grape Flowering Pathway Based on the Grape EST Database[J]. ACTA HORTICULTURAE SINICA, 2014, 41(1): 26-34.

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Forecasting Analysis of the Grape Flowering Pathway Based on the Grape EST Database

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