The Effect of UV-C Irradiation on Spatial and Temporal Accumulation of Flavanols and the Activity，Tissue Localization of LAR in Grape Berry

ACTA HORTICULTURAE SINICA ›› 2013, Vol. 40 ›› Issue (7): 1251-1261.

• Fruit Trees • Previous Articles Next Articles

The Effect of UV-C Irradiation on Spatial and Temporal Accumulation of Flavanols and the Activity，Tissue Localization of LAR in Grape Berry

WEN Peng-Fei, NIU Xing-Yan, XING Yan-Fu, NIU Tie-Quan, GAO Mei-Ying, JI Zheng-Chun, LI Chang-Heng, DU Li-Juan

College of Horticulture，Shanxi Agricultural University，Taigu，Shanxi 030801，China

Received:2013-03-04 Revised:2013-06-18 Online:2013-07-25 Published:2013-07-25

Abstract

Abstract: The 5-year old grapevine（Vitis vinifera L.‘Cabernet Sauvignon’）was subjected to the periodical UV-C irradiation during berry development. The spatial and temporal accumulation of flavanols and the enzyme activity of LAR in the berry were analyzed by spectrophotometer method，and the tissue localization of LAR1 and LAR2 were detected by the immunohistochemical localization. The results indicated that the accumulation of total phenol，flavanols，flavan-3-ols，as well as the LAR enzyme activity in the skin and flesh were induced by UV-C irradiation，which was irradiation dose-，organ/tissue-，and development stage-depend. There were no obvious changes in the tissue localization of LAR1 and LAR2 induced by UV-C，whereas an significant increasing signal was found，especially in the skin and vascular bundle. All the results suggest that UV-C irradiation could induce the accumulation of LAR1 and LAR2 enzyme protein in the skin and vascular bundle，an increasing in LAR activity in the skin during grape berry development，which resulted in the accumulation of total flavan-3-ols and flavanols.

Key words: grape, berries, UV-C, total flavan-3-ols, flavanols, LAR, tissue localization, Pepper mild mottle virus, Capsicum, in situ RT-PCR, digoxin, location in tissue, cDNA probe

CLC Number:

S 663.1

WEN Peng-Fei, NIU Xing-Yan, XING Yan-Fu, NIU Tie-Quan, GAO Mei-Ying, JI Zheng-Chun, LI Chang-Heng, DU Li-Juan. The Effect of UV-C Irradiation on Spatial and Temporal Accumulation of Flavanols and the Activity，Tissue Localization of LAR in Grape Berry[J]. ACTA HORTICULTURAE SINICA, 2013, 40(7): 1251-1261.

[1]	WANG Xiaochen, NIE Ziye, LIU Xianju, DUAN Wei, FAN Peige, and LIANG Zhenchang, . Effects of Abscisic Acid on Monoterpene Synthesis in‘Jingxiangyu’Grape Berries [J]. Acta Horticulturae Sinica, 2023, 50(2): 237-249.
[2]	CUI Jian, ZHONG Xionghui, LIU Zeci, CHEN Denghui, LI Hailong, HAN Rui, YUE Xiangqing, KANG Jungen, WANG Chao. Construction of Cabbage Chromosome Segment Substitution Lines [J]. Acta Horticulturae Sinica, 2023, 50(1): 65-78.
[3]	LIU Yiping, NI Menghui, WU Fangfang, LIU Hongli, HE Dan, KONG Dezheng. Association Analysis of Organ Traits with SSR Markers in Lotus（Nelumbo nucifera） [J]. Acta Horticulturae Sinica, 2023, 50(1): 103-115.
[4]	WANG Baoliang, LIU Fengzhi, JI Xiaohao, WANG Xiaodi, SHI Xiangbin, ZHANG Yican, LI Peng, and WANG Haibo. A New Early Ripening Grape Cultivar‘Huapu Zaoyu’for Table [J]. Acta Horticulturae Sinica, 2022, 49(S2): 33-34.
[5]	WANG Baoliang, WANG Haibo, JI Xiaohao, WANG Xiaodi, SHI Xiangbin, WANG Zhiqiang, WANG Xiaolong, and LIU Fengzhi. A New Middle Ripening Grape Cultivar‘Huapu Huangyu’for Table [J]. Acta Horticulturae Sinica, 2022, 49(S2): 35-36.
[6]	A Late-maturing Seedless Grape Cultivar‘Zilongzhu’. A Late-maturing Seedless Grape Cultivar‘Zilongzhu’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 37-38.
[7]	SHI Xiaoxin, DU Guoqiang, YANG Lili, QIAO Yuelian, HUANG Chengli, WANG Suyue, ZHAO Yuexin, WEI Xiaohui, WANG Li, and QI Xiangli. A Late-ripening Seedless Grape Cultivar‘Hongfeng Wuhe’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 39-40.
[8]	WU Yueyan, CHEN Tianchi, WANG Liru, HAN Shanqi, and FU Tao. A New Table Grape Cultivar‘Yongzaohong’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 41-42.
[9]	DENG Chaojun, CHEN Xiuping, XU Qizhi, JIANG Jimou, ZHENG Shaoquan, HU Wenshun, JIANG Fan, XU Jiahui, SU Wenbing, ZHANG Yaling, and HUANG Jingfeng. A New Longan Cultivar‘Fuxiang’with Rich Aroma [J]. Acta Horticulturae Sinica, 2022, 49(S2): 77-78.
[10]	CAO Yacong, ZHANG Zhenghai, YU Hailong, FENG Xigang, ZHANG Baoxi, and WANG Lihao. A New Spiral Pepper Cultivar‘Zhongjiao 409’ [J]. Acta Horticulturae Sinica, 2022, 49(S2): 111-112.
[11]	LI Zhengli, ZHANG Li, and MA Licang. A New Capsicum frutescens Cultivar‘Huangla Chaotian’with Yellow Fruit [J]. Acta Horticulturae Sinica, 2022, 49(S2): 123-124.
[12]	LIU Tingfu, LI Hanmei, WANG Linlin, and ZHONG Yangmin, . A New Vegetable Broad Bean Cultivar‘Lican 3’with High Yield and Large Grain [J]. Acta Horticulturae Sinica, 2022, 49(S2): 137-138.
[13]	WANG Xiaoyue, YAN Ailing, ZHANG Guojun, WANG Huiling, REN Jiancheng, LIU Zhenhua, SUN Lei, and XU Haiying, . A New Grape Cultivar‘Ruidu Wanhong’ [J]. Acta Horticulturae Sinica, 2022, 49(S1): 29-30.
[14]	QIN Gaihua, LIU Chunyan, LI Jiyu, and XU Yiliu, . A New Pomegranate Cultivar‘Suzi’ [J]. Acta Horticulturae Sinica, 2022, 49(S1): 39-40.
[15]	New Spring Cabbage Cultivars‘Zhonggan ’and‘Zhonggan ’. New Spring Cabbage Cultivars‘Zhonggan 27’and‘Zhonggan 28’ [J]. Acta Horticulturae Sinica, 2022, 49(S1): 63-64.

The Effect of UV-C Irradiation on Spatial and Temporal Accumulation of Flavanols and the Activity，Tissue Localization of LAR in Grape Berry

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments