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ACTA HORTICULTURAE SINICA ›› 2012, Vol. 39 ›› Issue (7): 1313-.

• Vegetables • Previous Articles     Next Articles

Construction of Genomic Fosmid Library of Brassica juncea and Screening of Cytological Markers for B-genome Chromosomes

PENG  Yuan-Feng, MENG  De-Xuan, HUANG  Yu-Bi, WANG  Gui-Xiang, LIU   Fan   

  1. Maize Research Institute of Sichuan Agricultural University,Wenjiang,Sichuan 611130,China;Beijing VegetableResearch Center,Beijing 100097,China;China National Maize Improvement Center of China Agricultural University,Beijing 100193,Chinan
  • Online:2012-07-25 Published:2012-07-25

Abstract: In this study,an unbiased Fosmid library of Brassica juncea was established,which consisted of 60 000 clones with 100% inserting frequency. In the constructed library,the size of average inserts was approximately 32 kb,corresponding to 1.8 genome equivalents. Subsequently,two types of
B-genome cytological markers were identified by means of library screening and chromosome fluorescence in situ hybridization(FISH). One type was that the signal located on all chromosomes,and the other one was that the signal was detected only on one pair of chromosomes.

Key words: Brassica juncea, fosmid library, cytological marker, molecular marker, FISH

CLC Number: