The Cryopreservation of Potato Pollen

ACTA HORTICULTURAE SINICA ›› 2003, Vol. 30 ›› Issue (6): 683-686.

• 研究论文 • Previous Articles Next Articles

The Cryopreservation of Potato Pollen

Wang Yuping ， Zhang Feng2
， and W an g Di

( D,raame~ of Horticulture，CrollSlZ Agricmurd { ，， ou 730070，China； College ofAgronomy，Cro1~lz Agric~urdUrao~ay，叽 u 730070，China)

Received:2003-02-11 Revised:2003-08-03 Online:2003-12-25 Published:2003-12-25

Abstract

Abstract: Abstract：The factors of afecting the viability of potato (Solanum tuberosum ) pollen stored in liqiud
nitrogen(LN)were investigated in order to improve cuopmse~ation effect．The msuhs indicated that desiccation
duration of 18 h and pre．freezing treatment at varied tempomtum of 0cI二(12 h) 一10cI二(12 h) 一20cI二
(12 h)can greatly increase the viability of polen stored in LN before thrown into LN．111e method of step by step
defrozen of一20oC (12 h)一4oC (12 h)一25oC (12 h)is better than that of defrozing pollen directly in 35
— 4ocI二or in (25±2)℃ ；The cuopmse~ation enhances pollen germination of some varieties．

Key words: Germplasm, Potato, Pollen, Cryopreservation

CLC Number:

s 532

Wang Yuping;Zhang Feng;and W an g Di. The Cryopreservation of Potato Pollen[J]. ACTA HORTICULTURAE SINICA, 2003, 30(6): 683-686.

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The Cryopreservation of Potato Pollen

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