Identification of Molecular Markers Linked to Foliage Non-bitterness Gene（bi）in Cucumis sativus L.

ACTA HORTICULTURAE SINICA ›› 2007, Vol. 34 ›› Issue (5): 1177-1182.

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Identification of Molecular Markers Linked to Foliage Non-bitterness Gene（bi）in Cucumis sativus L.

CHI Xiu-rong;GU Xing-fang^*;ZHANG Sheng-ping; WANG Xiao-wu;WANG Ye

(Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China)

Received:1900-01-01 Revised:1900-01-01 Online:2007-10-25 Published:2007-10-25

Abstract

Abstract: In this study, AFLP technique and the method of bulked segregant analysis were used to study the F₂ progeny of purified cucumber line 9110Gt（bibi）×03828（BiBi）.One AFLP molecular markers TG/GCT₁₅₀ linked to non-bitterness gene in cucumber with a genetic distance 6.43 cM were identified from 256 primer combinations. This band was collected and sequenced to synthesize a sequence characterized amplified region (SCAR) primer, and TG/GCT₁₅₀ was converted to SCAR marker SC₈₇ successfully.

Key words: Cucumber, Bitterness, Molecular marker, AFLP, SCAR

CHI Xiu-rong;GU Xing-fang;ZHANG Sheng-ping;WANG Xiao-wu;WANG Ye. Identification of Molecular Markers Linked to Foliage Non-bitterness Gene（bi）in Cucumis sativus L.[J]. ACTA HORTICULTURAE SINICA, 2007, 34(5): 1177-1182.

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Identification of Molecular Markers Linked to Foliage Non-bitterness Gene（bi）in Cucumis sativus L.

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