Abstract: In order to investigate the flowering repressor geneFLC in Chinese cabbage-pak-choi, by homologous cloning and RACE, vernalizationrelated gene BcFLC1 andBcFLC2 were respectively isolated from Brassica campestris ssp. chinensis var. communis ‘Shanghai Qing’ andBrassica campestris ssp. chinensis var. parachinensis ‘Sijiu’. The results of alignment indicated that there was no difference between the coding sequences (CDS) of BcFLC1 and BcFLC2, and the nucleotide sequence of promoter and intron 1 of them ere of no substantial difference. RTPCR analysis showed that the expression of BcFLC1 in the shoot apical meristem of ‘Shanghai Qing’was reduced gradually by the time of artificially vernalization at 4℃, and it got a very low level after 20 days and was undetectable after 30 days. The paraffin section also proved that 20 days cold treatment was enough for the apical meristem transforming to reproductive development. BcFLC2 was strongly expressed in the leaf of flowering Chinese cabbage‘Sijiu’ before floral buds appear; however, the expression of BcFLC2 was undetectable after floral buds’ appearance.

Key words: Chinese cabbage-pak-choi, Brassica campestris L. ssp. chinensis var. communis, Flowering Chinese cabbage, Brassica campestris L.ssp. chinensis var. parachinensis, Vernalization, FLC gene