Identification of SCAR Markers Linked to Orange Head Leaf Gene inChinese Cabbage ( Brassica rapa L. ssp. pekinensis)

ACTA HORTICULTURAE SINICA ›› 2007, Vol. 34 ›› Issue (1): 217-220.

• 研究简报 • Previous Articles Next Articles

Identification of SCAR Markers Linked to Orange Head Leaf Gene inChinese Cabbage ( Brassica rapa L. ssp. pekinensis)

WANG Guo-chen^1,2; ZHANG Feng-lan^1*
;YU Yang-jun¹; ZHANG De-shuang¹ ; ZHAO Xiu-yun¹

( ¹ National Engineering Research Center for Vegetables, Beijing 100097, China; ² College of Life Science, CapitalNormal University, Beijing 100037, China)

Received:1900-01-01 Revised:1900-01-01 Online:2007-02-25 Published:2007-02-25

Abstract

Abstract: Bulked segregant analysis was emp loyed to identify molecular markers linked to orange head
leaf gene ( or) trait based on RAPD and AFLP in Chinese cabbage with a doubled hap loid (DH) population
derived from the F1 between the two parents 91-112 (white head leaf) and T12-19 ( orange head leaf) via
microspore culture. One RAPD marker S1338₆₅₆ and one AFLP marker P67M54₁₇₂ were identified to be linked
to or gene with the distance of 8 cM and 13 cM respectively. These two markerswere successfully transformed
into SCAR markers SCOR₂₀₄ and SCOR₁₂₇. The accuracy of 90% and 89.1% were obtained when 110 differ
ent incompatibility breeding lines of Chinese cabbage were used to be investigated with two SCAR markers, in
dicating that these two SCAR makers could be used in marker2assisted2selection in orange head leaf breeding
p rograms and positional cloning of or gene in Chinese cabbage.

Key words: Chinese cabbage, Brassica rapa L. ssp. pekinensis, Orange head leaf gene (or), BSA, SCAR, Marker-assisted selection

WANG Guo-chen;ZHANG Feng-lan;YU Yang-jun;ZHANG De-shuang;ZHAO Xiu-yun. Identification of SCAR Markers Linked to Orange Head Leaf Gene inChinese Cabbage ( Brassica rapa L. ssp. pekinensis)[J]. ACTA HORTICULTURAE SINICA, 2007, 34(1): 217-220.

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Identification of SCAR Markers Linked to Orange Head Leaf Gene inChinese Cabbage ( Brassica rapa L. ssp. pekinensis)

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