Preliminary Analysis and Selection of Parental Combining Ability of Hybrid Progenies of Betula pendula‘Purple Rain’

doi:10.16420/j.issn.0513-353x.2023-0665

Acta Horticulturae Sinica ›› 2024, Vol. 51 ›› Issue (11): 2565-2574.doi: 10.16420/j.issn.0513-353x.2023-0665

• Genetic & Breeding·Germplasm Resources·Molecular Biology • Previous Articles Next Articles

Preliminary Analysis and Selection of Parental Combining Ability of Hybrid Progenies of Betula pendula‘Purple Rain’

ZHANG Jie¹, WEN Haoyu¹, MA Lixin², ZHU Qingchao², LIU Long³, JIANG Jing¹, and LIU Guifeng¹^,^*()

¹ National Key Laboratory of Forest Tree Genetics and Breeding，College of Forestry，Northeast Forestry University，Harbin 150040，China
² Nei Mongol Alongshan Forestry Bureau，Genhe，Inner Mongolia 022362，China
³ Mohe Dalinhe National Wetland Park Management Bureau，Mohe，Heilongjiang 165399，China

Received:2024-03-18 Revised:2024-08-20 Online:2024-12-12 Published:2024-11-25
Contact: and LIU Guifeng

Abstract

Abstract:

This study focused on the hybrid progeny of Betula pendula‘Purple Rain’from 20 five-year-old hybrid combinations，measuring traits such as growth，leaf color，and survival rate. The combining ability and effect values of these traits were analyzed using WinNCⅡsoftware. A comprehensive evaluation of the traits was conducted by applying the membership function method in conjunction with principal component analysis，leading to the selection of superior parents and hybrid combinations. Variance analysis revealed that traits such as ground diameter，leaf color，and survival rate showed significant（P < 0.05）or highly significant（P < 0.01）differences among the progeny families of different hybrid combinations. Variance analysis of combining ability effect values indicated that the variance of general combining ability（GCA）effect values for maternal traits was significant or highly significant，while only the variance of the paternal trait effect value for leaf color saturation（a^*）was highly significant. The GCA analysis suggested that the additive effects of the maternal parent were generally greater than those of the paternal parent，with variance components ranging from 0.94% to 84.16%. The analysis of specific combining ability（SCA）effects showed that SCA had the strongest influence on a^*，accounting for 82.30%. Based on the combining ability effect values for growth，leaf color，and survival rate traits in the hybrid progeny，M5 and M1-34 were selected as superior paternal parents，S3 as a superior maternal parent，and SZ-4 × M1 and S3 × M5 as superior hybrid combinations.

Key words: Betula, leaf color, growth trait, combining ability

ZHANG Jie, WEN Haoyu, MA Lixin, ZHU Qingchao, LIU Long, JIANG Jing, and LIU Guifeng. Preliminary Analysis and Selection of Parental Combining Ability of Hybrid Progenies of Betula pendula‘Purple Rain’[J]. Acta Horticulturae Sinica, 2024, 51(11): 2565-2574.

Figures/Tables 8

References 33

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母本 Female	父本 Male
母本 Female	M1	M2	M3	M4	M5	M1-34	M2-18
SZ	√（1801）	√（1802）	√（1803）	√（1804）	√（1805）	√（1806）	√（1807）
SZ-4	√（1808）	√（1809）	√（1810）	√（1811）	√（1812）	√（1813）	√（1814）
S3	×（1815）	√（1816）	√（1817）	√（1818）	√（1819）	√（1820）	√（1821）

母本 Female	父本 Male
母本 Female	M1	M2	M3	M4	M5	M1-34	M2-18
SZ	√（1801）	√（1802）	√（1803）	√（1804）	√（1805）	√（1806）	√（1807）
SZ-4	√（1808）	√（1809）	√（1810）	√（1811）	√（1812）	√（1813）	√（1814）
S3	×（1815）	√（1816）	√（1817）	√（1818）	√（1819）	√（1820）	√（1821）

变异来源 Source of variation	自由度 df	均方 MS	期望均方 EMS
母本一般配合力General combining ability of female	n₁-1	M_gk	σ_e² + bσ_s² + bn₂σ_gk²
父本一般配合力General combining ability of male	n₂-1	M_gj	σ_e² + bσ_s² + bn₁σ_gk²
特殊配合力Special combining ability	(n₁-1)( n₂-1)	M_sgj	σ_e² + bσ_s²
机误Error	(b-1)( n₁n₂-1)	M_e	σ_e²

变异来源 Source of variation	自由度 df	均方 MS	期望均方 EMS
母本一般配合力General combining ability of female	n₁-1	M_gk	σ_e² + bσ_s² + bn₂σ_gk²
父本一般配合力General combining ability of male	n₂-1	M_gj	σ_e² + bσ_s² + bn₁σ_gk²
特殊配合力Special combining ability	(n₁-1)( n₂-1)	M_sgj	σ_e² + bσ_s²
机误Error	(b-1)( n₁n₂-1)	M_e	σ_e²

变异来源 Source of variation	df	树高 Hight	地径 Diameter	花色苷相对含量 Relative content of anthocyanin	叶绿素相对含量 Relative content of chlorophyll	a^*	b^*	存活率 Survival rate
区组 Block	3	11.021	692.493^**	4.844^**	1.777	5.879^**	0.063	0.570
父本 Male（P1）	6	8.249	431.400	0.655	1.330	7.942^**	1.708	0.887
母本 Female（P2）	2	4.953	314.331^*	5.688^**	15.743^**	5.362^**	10.737^**	5.034^*
P2 × P1	12	19.320	468.721^*	2.132^*	1.906^*	6.581^**	1.590	0.849

Preliminary Analysis and Selection of Parental Combining Ability of Hybrid Progenies of Betula pendula‘Purple Rain’

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配合力组分 Component of combining ability	地径 Diameter	花色苷相对含量 Relative content of anthocyanin	叶绿素相对含量 Relative content of chlorophyll	a^*	b^*	存活率 Survival rate
父本一般配合力方差 Male variance of general combining ability	2.44	0.54	0.70	1.45	0.07	1.21
父本一般配合力方差 Male variance of general combining ability	（33.89%）	（8.34%）	（8.82%）	（16.75%）	（30.30%）	（5.00%）
母本一般配合力方差 Female variance of general combining ability	4.09	3.17	2.58	0.08	0.16	20.30
母本一般配合力方差 Female variance of general combining ability	（56.80%）	（48.76%）	（32.26%）	（0.94%）	（67.96%）	（84.16%）
一般配合力方差 Variance of general combining ability	6.53	3.71	3.28	1.53	0.24	21.50
一般配合力方差 Variance of general combining ability	（90.68%）	（57.10%）	（41.08%）	（17.70%）	（98.27%）	（89.16%）
父本 × 母本特殊配合力方差 Male × female variance of specific combining ability	0.67	2.79	4.70	7.11	0.00	2.62
	（9.32%）	（42.90%）	（58.92%）	（82.30%）	（1.73%）	（10.84%）
环境方差 Environmental variance	7.20	14.83	5.22	6.38	0.28	93.47
家系遗传力 H²	（15.28%）	（30.47%）	（60.47%）	57.53%	46.29%	20.51%

亲本Parents	材料代号 Material code	地径 Diameter	花色苷相对含量 Relative content of anthocyanin	叶绿素相对含量 Relative content of chlorophyll	a^*	b^*	存活率 Survival rate
父本 Male	M1	-0.797 abc	-0.072 a	-0.027 ab	2.618 a	0.084 ab	-1.364 a
	M2	-1.067 bc	0.294 a	-0.076 ab	-0.569 b	-0.362 b	4.552 a
	M3	1.129 ab	-1.566 a	0.533 ab	-0.851 b	0.170 ab	0.315 a
	M4	0.518 abc	-1.040 a	-1.040 b	-0.760 b	0.574 a	-6.363 a
	M5	-2.174 c	2.712 a	1.748 a	-0.666 b	-0.194 b	-2.424 a
	M1-34	0.067 abc	0.329 a	0.103 ab	-0.027 b	-0.114 b	4.462 a
	M2-18	2.324 a	-0.655 a	-1.241 b	0.255 b	-0.160 b	0.823 a
母本 Famale	SZ	-0.758 b	0.085 a	-2.110 b	-0.174 b	-0.372 b	3.253 b
	SZ-4	-0.788 b	3.143 a	1.633 a	0.848 a	-0.154 b	-8.626 b
	S3	1.546 a	-3.228 b	0.477 a	-0.674 b	0.525 a	5.373 a

亲本Parents		地径 Diameter	花色苷相对含量Relative content of anthocyanin	叶绿素相对含量Relative content of chlorophyll	a^*	b^*	存活率 Survival rate	均值 Mean	排名 Ranking
父本 Male	M1	0.028	0.078	0.080	0.209	0.018	0.110	0.087	3
	M2	0.022	0.097	0.077	0.017	0.000	0.241	0.076	4
	M3	0.066	0	0.117	0	0.022	0.148	0.059	6
	M4	0.054	0.027	0.013	0.005	0.038	0.000	0.023	7
	M5	0	0.224	0.198	0.011	0.007	0.087	0.088	2
	M1-34	0.045	0.099	0.089	0.050	0.010	0.239	0.089	1
	M2-18	0.090	0.048	0	0.067	0.008	0.159	0.062	5
母本 Famale	SZ	0.003	0.024	0	0.021	0	0.171	0.037	3
	SZ-4	0	0.046	0.178	0.063	0.062	0.000	0.058	2
	S3	0.255	0	0.123	0	0.255	0.202	0.139	1

杂交组合 Hybridized combination	地径 Diameter	花色苷相对含量 Relative content of anthocyanin	叶绿素相对含量 Relative content of chlorophyll	a^*	b^*	存活率 Survival rate
SZ × M1	1.008 ab	-0.904 bcd	-1.366 bc	-2.997 f	0.358 abc	-4.345 b
SZ-4 × M1	-1.008 b	0.904 bcd	1.366 abc	2.997 a	-0.358 cde	4.345 ab
SZ × M2	-1.519 b	2.150 abc	0.958 abc	0.843 bc	-0.063 abcde	-5.345 b
SZ-4 × M2	0.374 ab	2.638 ab	0.410 abc	-0.729 e	-0.442 de	-3.285 ab
S3 × M2	1.145 ab	-4.788 de	-1.369 bc	-0.114 cde	0.505 a	8.630 a
SZ × M3	1.292 ab	1.392 abcd	-1.944 bc	1.062 b	0.167 abcde	1.507 ab
SZ-4 × M3	0.402 ab	-3.492 cde	0.419 abc	-1.361 e	0.621 a	7.053 ab
S3 × M3	-1.693 b	2.100 abcd	1.524 ab	0.299 bcde	-0.788 e	-8.560 b
SZ × M4	-1.447 b	1.007 bcd	-0.642 abc	1.162 b	-0.489 de	-0.241 ab
SZ-4 × M4	0.941 ab	-1.229 bcde	-0.820 abc	-0.297 cde	-0.011 abcde	-1.953 ab
S3 × M4	0.506 ab	0.222 bcd	1.462 abc	-0.866 e	0.499 ab	2.193 ab
SZ × M5	2.793 a	-6.497 e	-0.647 abc	0.153 bcde	0.245 abcd	-3.267 ab
SZ-4 × M5	-2.978 b	7.415 a	-0.153 abc	-0.153 cde	-0.409 cde	-0.461 ab
S3 × M5	0.185 ab	-0.918 bcd	0.800 abc	0.000 bcde	0.163 abcde	3.728 ab
SZ × M1-34	-0.844 b	-0.176 bcd	1.488 ab	-0.108 bcde	-0.130 abcde	4.642 ab
SZ-4 × M1-34	0.841 ab	-2.028 bcde	0.566 abc	-0.539 de	0.381 abc	-4.862 b
S3 × M1-34	0.003 ab	2.203 ab	-2.054 c	0.648 bcd	-0.251 bcde	0.220 ab
SZ × M2-18	-1.283 b	3.027 ab	2.153 a	-0.115 cde	-0.089 abcde	7.050 ab
SZ-4 × M2-18	1.428 ab	-4.209 cde	-1.790 bc	0.082 bcde	0.218 abcde	-0.838 ab
S3 × M2-18	-0.145 ab	1.181 abcd	-0.364 abc	0.033 bcde	-0.129 abcde	-6.212 b

杂交组合 Hybridized combination	地径 Diameter	花色苷相对含量Relative content of anthocyanin	叶绿素相对含量Relative content of chlorophyll	a^*	b^*	存活率 Survival rate	均值 Mean	排名 Ranking
SZ × M1	0.017	0.153	0.043	0.000	0.024	0.053	0.073	20
SZ-4 × M1	0.009	0.203	0.212	0.184	0.016	0.163	0.197	2
SZ × M2	0.006	0.237	0.187	0.118	0.051	0.041	0.160	7
SZ-4 × M2	0.015	0.251	0.153	0.070	0.011	0.067	0.141	9
S3 × M2	0.018	0.047	0.043	0.089	0.043	0.217	0.114	16
SZ × M3	0.019	0.216	0.007	0.125	0.038	0.127	0.133	12
SZ-4 × M3	0.015	0.082	0.153	0.050	0.047	0.197	0.136	11
S3 × M3	0.006	0.236	0.222	0.101	0.000	0.000	0.141	8
SZ × M4	0.007	0.206	0.088	0.128	0.028	0.105	0.140	10
SZ-4 × M4	0.017	0.145	0.077	0.083	0.020	0.083	0.106	17
S3 × M4	0.015	0.184	0.218	0.066	0.047	0.136	0.167	5
SZ × M5	0.025	0.000	0.087	0.097	0.037	0.067	0.078	19
SZ-4 × M5	0.000	0.382	0.118	0.087	0.026	0.102	0.179	3
S3 × M5	0.014	0.153	0.177	0.092	0.035	0.155	0.156	6
SZ × M1-34	0.009	0.173	0.220	0.089	0.013	0.166	0.168	4
SZ-4 × M1-34	0.017	0.123	0.163	0.076	0.024	0.047	0.112	15
S3 × M1-34	0.013	0.239	0.000	0.112	0.014	0.111	0.122	13
SZ × M2-18	0.007	0.261	0.261	0.089	0.042	0.197	0.214	1
SZ-4 × M2-18	0.019	0.063	0.016	0.095	0.026	0.097	0.079	18
S3 × M2-18	0.012	0.211	0.105	0.093	0.035	0.030	0.121	14

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