Hormonal and Transcriptome Analysis of Ripe Fruits
of Non-Climacteric and Climacteric Melon

doi:10.16420/j.issn.0513-353x.2024-0371

Acta Horticulturae Sinica ›› 2025, Vol. 52 ›› Issue (4): 883-896.doi: 10.16420/j.issn.0513-353x.2024-0371

• Genetic & Breeding · Germplasm Resources · Molecular Biology • Previous Articles Next Articles

Hormonal and Transcriptome Analysis of Ripe Fruits of Non-Climacteric and Climacteric Melon

TANG Lingli^1,2，HE Yuhua¹，WANG Qingtao¹，AN Lulu¹，XU Yongyang¹，ZHANG Jian¹，KONG Weihu¹，HU Keyun¹，and ZHAO Guangwei^1,2,*(tanglingli@caas.cn)

¹Zhengzhou Fruit Research Institute，Chinese Academy of Agricultural Sciences，Zhengzhou 450009，China ²Zhongyuan Research Center，Chinese Academy of Agricultural Sciences，Xinxiang，Henan 453500，China

Received:2024-12-18 Revised:2025-02-19 Online:2025-04-25 Published:2025-04-25
Contact: ZHAO Guangwei

Abstract

Abstract: Melon can be divided into non-climacteric and climacteric according to mature habituation，but the most important hormones，sugar metabolites and genetic regulatory mechanisms remain elusive between non-climacteric and climacteric melon. Plant hormone metabolism detection revealed that abscisic acid（ABA），and the content of 1-aminocyclop ropanecarboxylic acid（ACC），trans-Zeatin Riboside-5′-monophosphate Disodium Salt（tZRMP）and auxin were higher in mature non-climacteric melon. The KEGG pathway enrichment analysis of these differentially expressed genes（DEGs）revealed“metabolic pathways”，“plant hormone signal transduction”and“biosynthesis of various alkaloids” were significantly different in the ripe fruits of non-climacteric and climacteric melons. The RNA-seq and qRT-PCR analyses were performed to identify important genes，enzymes and pathways that are involved in sugar and hormone metabolites. CmARF，CmARR2-like，CmGAI-like，CmPP2C，CmACO and CmEIL associated with plant hormone metabolism were identified in mature melon. ABA，Indole，ACC and GA pathways play key roles in non-climacteric melon. While besides ABA，IAA，GA，and ethylene（ETH）signaling pathways were also involved in regulating the maturation of climacteric melon.

Key words: melon, fruit ripening, phytohormone, transcriptome, gene expression

CLC Number:

S 652

TANG Lingli, HE Yuhua, WANG Qingtao, AN Lulu, XU Yongyang, ZHANG Jian, KONG Weihu, HU Keyun, ZHAO Guangwei. Hormonal and Transcriptome Analysis of Ripe Fruits of Non-Climacteric and Climacteric Melon[J]. Acta Horticulturae Sinica, 2025, 52(4): 883-896.

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Hormonal and Transcriptome Analysis of Ripe Fruits of Non-Climacteric and Climacteric Melon

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