Identification of Melon GolS Genes Family and the Expression Analysis in Response to Low Temperature Stress

doi:10.16420/j.issn.0513-353x.2024-0111

Acta Horticulturae Sinica ›› 2025, Vol. 52 ›› Issue (1): 136-148.doi: 10.16420/j.issn.0513-353x.2024-0111

• Genetic & Breeding·Germplasm Resources·Molecular Biology • Previous Articles Next Articles

Identification of Melon GolS Genes Family and the Expression Analysis in Response to Low Temperature Stress

HE Dandan, HE Hongtai, WANG Wenting, ZHOU Wenmei, LIU Yanmin, LIU Sushuang^*()

Department of Bioengineering，School of Life and Health Sciences，Huzhou College，Huzhou，Zhejiang 313000，China

Received:2024-04-10 Revised:2024-10-24 Online:2025-01-25 Published:2025-01-19
Contact: LIU Sushuang

Abstract

Abstract:

In order to study the characteristics of melon（Cucumis melo L.）GolS family genes and their responses to low temperature stress，members of the melon GolS family of genes were identified using bioinformatics. The chromosomal locations，evolutionary relationships，genetic structure，promoters，cis-acting elements，tissue-specific expressions and expression patterns under low temperature stress were predicted using bioinformatics. After exhaustive analysis，12 CmGolS genes were successfully identified and found to be unevenly distributed across nine chromosomes. The analyses of phylogenetic showed that these genes were divided into 5 groups. Physicochemical analysis showed that all CmGolS proteins were hydrophilic proteins. The CmGolS genes were highly conserved and they have 2-5 introns. These genes contain conserved motifs“DxD”“HxxGxxKPW”and many cis-acting elements，which related to stress and hormones such as gibberellin，auxin and abscisic acid. In addition CmGolS genes have tissue specific expressions，most of them are expressed in male flowers. And the relative expression of most of them show a significant up-regulation trend under low temperature stress that indicated CmGolS genes can actively participate in the processes of responding to low temperature stress.

Key words: melon, galactinol synthase, bioinformatics analysis, cold stress, expression analysis

HE Dandan, HE Hongtai, WANG Wenting, ZHOU Wenmei, LIU Yanmin, LIU Sushuang. Identification of Melon GolS Genes Family and the Expression Analysis in Response to Low Temperature Stress[J]. Acta Horticulturae Sinica, 2025, 52(1): 136-148.

Figures/Tables 8

References 41

[1]	Cao Chenji, Fang Qianhai, Xu Fei, He Zhengquan, Yu Lulu. 2022. Bioinformatics analysis and subcellular localization of AtGolS2 protein. Molecular Plant Breeding, https://kns.cnki.net/kcms/detail/46.1068.S.20221007.1549.004.html. (in Chinese)
	曹宸基, 方钱海, 徐飞, 何正权, 余璐璐. 2022. AtGolS2蛋白的生物信息学分析及亚细胞定位. 分子植物育种, https://kns.cnki.net/kcms/detail/46.1068.S.20221007.1549.004.html.
[2]	Chu D H, Melanie B, Le T D. 2018. Functional characterisation of a soybean galactinol synthase gene under various stress conditions. Vietnam Journal of Science. Technology and Engineering, 60 (63):33-36.
[3]	Cong Qing, Cheng Longjun, Yang Ning. 2019. Physiological function and regulation mechanism of galactinol synthase in plants. Chinese Journal of Biochemistry and Molecular Biology, 35 (11):1193-1200. (in Chinese) doi: 10.13865/j.cnki.cjbmb.2019.11.03
	从青, 程龙军, 杨宁. 2019. 植物肌醇半乳糖苷合酶的生理功能和调控机制. 中国生物化学与分子生物学报, 35 (11):1193-1200. doi: 10.13865/j.cnki.cjbmb.2019.11.03
[4]	Cui L H, Byun M Y, Oh H G, Kim S J, Lee J, Park H, Lee H, Kim W T. 2020. Poaceae Type II Galactinol Synthase2 from antarctic flowering plant Deschampsia antarctica and rice improves cold and drought tolerance by accumulation of Raffinose Family Oligosaccharides in transgenic rice plants. Plant & Cell Physiology, 61 (1):88-104.
[5]	Dai H, Zhu Z, Wang Z, Zhang Z, Kong W, Miao M. 2022. Galactinol synthase 1 improves cucumber performance under cold stress by enhancing assimilate translocation. Horticulture Research, 9:unhab063.
[6]	Dai Haibo. 2023. The mechanism of raffinose family oligosaccharide metabolism regulating seed vigor,low-temperature adaptation and source-sink allocation in cucumber[Ph. D. Dissertation]. Yangzhou: Yangzhou University. (in Chinese)
	戴海博. 2023. 黄瓜棉子糖家族寡糖代谢调控种子活力、低温适应和源库分配的机制[博士论文]. 扬州: 扬州大学.
[7]	ElSayed A I, Rafudeen M S, Golldack D. 2014. Physiological aspects of raffinose family oligosaccharides in plants:protection against abiotic stress. Plant Biology(Stuttgart,Germany), 16 (1):1-8.
[8]	Fan Y, Yu M, Liu M, Zhang R, Sun W, Qian M, Duan H, Chang W, Ma J, Qu C, Zhang K, Lei B, Lu K. 2017. Genome-Wide identification,evolutionary and expression analyses of the galactinol synthase gene family in rapeseed and tobacco. International Journal of Molecular Sciences, 18 (12):2768.
[9]	Gao Qinghai, Wang Yakun, Guo Yuanyuan. 2016. Identification for tolerance to low temperature and weak light and selection for morphological indexes of melon at seedling stage. Acta Agriculturae Zhejiangensis, 28 (8):1360-1367. (in Chinese)
	高青海, 王亚坤, 郭远远. 2016. 薄皮甜瓜种质资源苗期耐低温弱光鉴定及形态指标选择. 浙江农业学报, 28 (8):1360-1367.
[10]	Guan Y, Hwarari D, Korboe H M, Ahmad B, Cao Y, Movahedi A, Yang L. 2023. Low temperature stress-induced perception and molecular signaling pathways in plants. Environmental and Experimental Botany,(207):105190.
[11]	Hao J H, Yang R, Fang K F, Wang J L, Zhang Q, Shen Y Y, Li T L. 2014. Low night temperatures inhibit galactinol synthase gene expression and phloem loading in melon leaves during fruit development. Russian Journal of Plant Physiology, 61 (2):178-187.
[12]	He F, Xu J, Jian Y, Duan S, Hu J, Jin L, Li G. 2023. Overexpression of galactinol synthase1 from Solanum commersonii(ScGolS1)confers freezing tolerance in transgenic potato. Horticultural Plant Journal, 9 (3):541-552.
[13]	He Hongyun, Xue Lin, Tian Liping, Chen Yuanliang. 2008. Effect of low temperature stress on root vigor and osmotic adjustment matter in Muskmelon seedlings. Journal of Shihezi University(Natural Science), 26 (5):583-586. (in Chinese)
	和红云, 薛琳, 田丽萍, 陈远良. 2008. 低温胁迫对甜瓜幼苗根系活力及渗透调节物质的影响. 石河子大学学报(自然科学版), 26 (5):583-586.
[14]	Jing Q, Chen A, Lü Z, Dong Z, Wang L, Meng X, Feng Y, Wan Y, Su C, Cui Y, Xu W, Hou H, Zhu X. 2023. Systematic analysis of galactinol synthase and raffinose synthase gene families in potato and their expression patterns in development and abiotic stress responses. Genes, 14 (7):1344.
[15]	Li Lili. 2023. Mining cold-tolerance related genes and functional verification in melon[Ph. D. Dissertation]. Zhengzhou: Henan Agricultural University. (in Chinese)
	李丽丽. 2023. 甜瓜耐冷相关基因的挖掘及功能验证[博士论文]. 郑州: 河南农业大学.
[16]	Li R, Yuan S, He Y, Fan J, Zhou Y, Qiu T, Lin X, Yao Y, Liu J, Fu S, Hu X, Guo J. 2018. Genome-wide identification and expression profiling analysis of the galactinol synthase gene family in Cassava(Manihot esculenta Crantz). Agronomy, 8 (11):250.
[17]	Li Tianjie, Zhang Weihua, Gao Ying, Wang Xiaoyu, Gao Longfei, Liang Xing, Pan Qi, Wu Ying. 2023. Genome-wide identification and abiotic stress expression analysis of GolS gene family in Vaccinium corymbosum. Plant Physiology Journal, 59 (3):543-556. (in Chinese)
	李天杰, 张卫华, 高英, 王晓宇, 高龙飞, 梁兴, 潘祺, 吴颖. 2023. 蓝莓GolS基因家族鉴定及非生物胁迫下的表达模式分析. 植物生理学报, 59 (3):543-556.
[18]	Liu Dan, Wang Keai, Ni Peng, Wang Qiuyan, Zhu Kang, Wei Wenliang. 2022. Identification of soybean GolS gene family and analysis of expression patterns under salt and drought stresses. Chinese Journal of Biotechnology, 38 (10):3757-3772. (in Chinese) doi: 10.13345/j.cjb.220449 pmid: 36305408
	刘丹, 王柯蔼, 倪蓬, 王秋艳, 朱康, 危文亮. 2022. 大豆GolS基因家族鉴定及盐旱胁迫下的表达分析. 生物工程学报, 38 (10):3757-3772. pmid: 36305408
[19]	Liu J J, Odegard W, de Lumen B O. 1995. Galactinol synthase from kidney bean cotyledon and zucchini leaf(purification and N-Terminal sequences). Plant Physiology, 109 (2):505-511. pmid: 7480343
[20]	Ma S, Lv J, Li X, Ji T, Zhang Z, Gao L. 2021. Galactinol synthase gene 4(CsGolS4)increases cold and drought tolerance in Cucumis sativus L. by inducing RFO accumulation and ROS scavenging. Environmental and Experimental Botany, 185:104406.
[21]	Martins C P S, Fernandes D, Guimarães V M, Du D, Silva D C, Almeida A A F, Gmitter J F G, Otoni W C, Costa M G C. 2022. Comprehensive analysis of the galactinol synthase(GolS)gene family in citrus and the function of CsGolS6 in stress tolerance. PLoS ONE, 17 (9):e0274791.
[22]	Mukherjee S, Sengupta S, Mukherjee A, Basak P, Majumder A L. 2018. Abiotic stress regulates expression of galactinol synthase genes post-transcriptionally through intron retention in rice. Planta, 249 (3):891-912.
[23]	Persson K, Ly H D, Dieckelmann M, Wakarchuk W W, Withers S G, Strynadka N C. 2001. Crystal structure of the retaining galactosyltransferase LgtC from Neisseria meningitidis in complex with donor and acceptor sugar analogs. Nature Structural Biology, 8 (2):166-175. pmid: 11175908
[24]	Saimi Gulisitan, Liu Suiyunhao, Zheng Pei, Chen Yongkun, Huang Gengqing, Zhao Huixin, Zhang Jingbo. 2023. Identification of GolS gene family in Cotton(Gossypium spp.)and its function analysis in drought stress response. Journal of Agricultural Biotechnology, 31 (4):704-717. (in Chinese)
	古丽斯坦 · 赛米, 刘隋赟昊, 郑蓓, 陈永坤, 黄耿青, 赵惠新, 张经博. 2023. 棉花GolS基因家族的鉴定及其在干旱胁迫应答中的功能研究. 农业生物技术学报, 31 (4):704-717.
[25]	Sengupta S, Mukherjee S, Basak P, Majumder A L. 2015. Significance of galactinol and raffinose family oligosaccharide synthesis in plants. Frontiers in Plant Science, 6:656. doi: 10.3389/fpls.2015.00656 pmid: 26379684
[26]	Shen Yang, Jia Bowei, Wang Jinyu, Cai Xiaoxi, Hu Bingshuang, Wang Yan, Chen Yue, Sun Mingzhe, Sun Xiaoli. 2021. Functional analysis of Arabidopsis thaliana galactinol synthase AtGolS2 in response to abiotic stress. Molecular Plant Breeding, 19 (11):3588-3597. (in Chinese)
	沈阳, 贾博为, 王金玉, 才晓溪, 胡冰霜, 王研, 陈悦, 孙明哲, 孙晓丽. 2021. 拟南芥肌醇半乳糖苷酶AtGolS2基因在非生物胁迫应答中的功能分析. 植物分子育种, 19 (11):3588-3597.
[27]	Smith P T, Kuo T M, Crawford C G. 1991. Purification and characterization of galactinol synthase from mature Zucchini Squash Leaves 1. Plant Physiology, 96 (3):693-698.
[28]	Sterling J D, Atmodjo M A, Inwood S E, Kumar K V S, Quigley H F, Hahn M G, Mohnen D. 2006. Functional identification of an Arabidopsis pectin biosynthetic homogalacturonan galacturonosyl transferase. Proceedings of the National Academy of Sciences of the United States of America, 103 (13):5236-5241.
[29]	Taji T, Ohsumi C, Iuchi S, Seki M, Kasuga M, Kobayashi M, Yamaguchi-S K, Shinozaki K. 2002. Important roles of drought- and cold-inducible genes for galactinol synthase in stress tolerance in Arabidopsis thaliana. The Plant Journal:for Cell and Molecular Biology, 29 (4):417-426.
[30]	Volk G M, Haritatos E E, Turgeon R. 2003. Galactinol synthase gene expression in melon. Journal of the American Society for Horticultural Science, 128 (1):8-15.
[31]	Wang Jie. 2023. Comprehensive evaluation of physiologicalresponse and cold resistance of different muskmelon germplasmon chillingstress[M. D. Dissertation]. Yangling: Northwest A & F University. (in Chinese)
	王婕. 2023. 不同甜瓜种质对低温胁迫的生理响应及耐寒性综合评价[硕士论文]. 杨凌: 西北农林科技大学.
[32]	Wiggins C A R, Munro S. 1998. Activity of the yeast MNN1 α-1,3-mannosyltransferase requires a motif conserved in many other families of glycosyltransferases. Proceedings of the National Academy of Sciences, 95 (14):7945-7950.
[33]	Wu Meimei, Zhang Xian, Zheng Junqian, Yang Xiaozhen. 2014. Research progress on cold resistance of watermelon and melon. China Cucurbits and Vegetables, 27 (S1):1-5. (in Chinese)
	吴梅梅, 张显, 郑俊鶱, 杨小振. 2014. 西瓜甜瓜抗寒性研究进展. 中国瓜菜, 27 (S1):1-5.
[34]	Yang J, Ling C, Liu Y, Zhang H, Hussain Q, Lyu S, Wang S, Liu Y. 2022. Genome-wide expression profiling analysis of kiwifruit GolS and RFS genes and identification of AcRFS4 function in raffinose accumulation. International Journal of Molecular Sciences, 23 (16):8836.
[35]	Yi Mengmeng. 2018. Molecular cloning and expression regulatory of AnGolS2 gene from Ammopiptan-thusmongolicus in Xinjiang[M. D. Dissertation]. Shenyang: Shenyang Agricultural University. (in Chinese)
	易萌萌. 2018. 新疆沙冬青AnGolS2基因的克隆及表达调控研究[硕士论文]. 沈阳: 沈阳农业大学.
[36]	Yin Y, Chen H, Hahn M G, Mohnen D, Xu Y. 2010. Evolution and function of the plant cell wall synthesis-related glycosyltransferase family 8. Plant Physiology, 153 (4):1729-1746. doi: 10.1104/pp.110.154229 pmid: 20522722
[37]	You J, Wang Y, Zhang Y, Dossa K, Li D, Zhou R, Wang L, Zhang X. 2018. Genome-wide identification and expression analyses of genes involved in raffinose accumulation in sesame. Scientific Reports, 8 (1):4331. doi: 10.1038/s41598-018-22585-2 pmid: 29531231
[38]	Yu Ruoqian, Wang Fuxiang, Zheng Yanmei, Lin Yuelong, Wei Yidong, Cai Qiuhua, Zhou Yuanchang, Zhang Jianfu. 2022. Research advances on the biological function of raffinose families oligosaccharides in seed vigor and abiotic stress. Fujian Journal of Agricultural Sciences, 37 (1):114-122. (in Chinese)
	余箬芊, 王福祥, 郑燕梅, 林悦龙, 魏毅东, 蔡秋华, 周元昌, 张建福. 2022. 植物棉子糖家族寡糖(RFOs)在种子活力及非生物胁迫中的生物学功能研究进展. 福建农业学报, 37 (1):114-122.
[39]	Zhang Ruiteng, Lü Jianchun, Zhou Mengdi, Liu Jinglin, Li ren, Guo Yangdong, Wang Huaisong, Fu Qiushi. 2018. Expression and function analysis of CmGAS1in melon. Acta Horticulturae Sinica, 45 (10):1929-1940. (in Chinese)
	张瑞腾, 吕建春, 周梦迪, 刘静霖, 李仁, 郭仰东, 王怀松, 付秋实. 2018. 甜瓜肌醇半乳糖苷合成酶基因CmGAS1的表达与功能分析. 园艺学报, 45 (10):1929-1940. doi: 10.16420/j.issn.0513-353x.2017-0766
[40]	Zhang Yongping, Diao Qiannan, Cao Yanyan, Shi Mengya, Zhang Hongmei, Jin Haijun, Jiang Shouyang, Shen Haibin. 2022. Effect of exogenous SNP on the growth and enzymes related to nitrogen metabolism of melon seedlings under cold stress. Molecular Plant Breeding, 20 (5):1692-1698. (in Chinese)
	张永平, 刁倩楠, 曹燕燕, 史梦雅, 张红梅, 金海军, 姜守阳, 沈海斌. 2022. 外源SNP对低温胁迫下甜瓜幼苗生长和氮代谢相关酶的影响. 植物分子育种, 20 (5):1692-1698.
[41]	Zhang Yongping, Xu Shuang, Yang Shaojun, Lu Shijun, Chen Youyuan. 2017. Effect of exogenous spermidine on the growth and antioxidant system of melon seedlings under low temperature stress. Plant Physiology Journal, 53 (6):1087-1096. (in Chinese)
	张永平, 许爽, 杨少军, 陆世钧, 陈幼源. 2017. 外源亚精胺对低温胁迫下甜瓜幼苗生长和抗氧化系统的影响. 植物生理学报, 53 (6):1087-1096.

基因 Gene	引物 Primer	序列（5′-3′） Sequence	大小/bp Size
CmGolS1	MELO3C015870.2.1	F2：GGAATCATCCGAAAACCTACCT；R2：ACACGAGAACACCTCCCCTT	234
CmGolS3	MELO3C010862.2.1	F2：CGAGAGGGAGATTGGTGGTT；R2：AGGAAGTTTGTTCGTCGTGGT	689
CmGolS4	MELO3C012925.2.1-F3	F3：GTCACCTCTCCCACTCCCTTT；R3：TTTGCATGTTCTCTTCTTTCCC	211
CmGolS5	MELO3C008614.2.1-F1	F1：AATTCTGGTGTTATGGTTGTGGA；R1：CTTCTTGTGGTAAGTTTGGCTCA	166
CmGolS7	MELO3C008045.2.1-F2	F2：TCAGGAACCCAAAAGCAGAAA；R2：GCGGCGTAGGTACGTAAAACT	783
CmGolS8	MELO3C022024.2.1	F1：GCCATTCGTCTTCATTCTCACT；R1：TTGGACTCCCTTATCTCCTTCTC	315
CmEFIa	CmEFIa	F：ACTGTGCTGTCCTCATTATTG；R：AGGGTGAAAGCAAGAAGAGC	98

基因 Gene	引物 Primer	序列（5′-3′） Sequence	大小/bp Size
CmGolS1	MELO3C015870.2.1	F2：GGAATCATCCGAAAACCTACCT；R2：ACACGAGAACACCTCCCCTT	234
CmGolS3	MELO3C010862.2.1	F2：CGAGAGGGAGATTGGTGGTT；R2：AGGAAGTTTGTTCGTCGTGGT	689
CmGolS4	MELO3C012925.2.1-F3	F3：GTCACCTCTCCCACTCCCTTT；R3：TTTGCATGTTCTCTTCTTTCCC	211
CmGolS5	MELO3C008614.2.1-F1	F1：AATTCTGGTGTTATGGTTGTGGA；R1：CTTCTTGTGGTAAGTTTGGCTCA	166
CmGolS7	MELO3C008045.2.1-F2	F2：TCAGGAACCCAAAAGCAGAAA；R2：GCGGCGTAGGTACGTAAAACT	783
CmGolS8	MELO3C022024.2.1	F1：GCCATTCGTCTTCATTCTCACT；R1：TTGGACTCCCTTATCTCCTTCTC	315
CmEFIa	CmEFIa	F：ACTGTGCTGTCCTCATTATTG；R：AGGGTGAAAGCAAGAAGAGC	98

名称 Name	ID	氨基酸数 Animo acid number	分子质量/kD	等电点pI	不稳定系数Coefficient of instability	平均亲水指数 Grand average of hydropathicity	亚细胞定位 Prediction of subcellular localization
CmGolS1	MELO3C015870.2.1	367	42.13	8.70	41.22	-0.274	叶绿体Chloroplast
CmGolS2	MELO3C008407.2.1	655	76.19	6.64	45.07	-0.368	叶绿体Chloroplast
CmGolS3	MELO3C010862.2.1	520	61.73	8.18	53.33	-0.482	细胞质Cytoplasm
CmGolS4	MELO3C012925.2.1	335	38.51	4.97	44.32	-0.291	细胞质Cytoplasm
CmGolS5	MELO3C008614.2.1	414	46.95	6.53	40.70	-0.033	质膜Plasma membrane
CmGolS6	MELO3C004270.2.1	614	71.32	8.32	57.16	-0.332	叶绿体Chloroplast
CmGolS7	MELO3C008045.2.1	567	65.91	6.37	40.38	-0.351	细胞质Cytoplasm
CmGolS8	MELO3C022024.2.1	534	61.53	8.53	43.13	-0.179	液泡Vacuole
CmGolS9	MELO3C012034.2.1	377	42.76	6.86	52.29	-0.156	液泡Vacuole
CmGolS10	MELO3C011991.2.1	332	38.00	5.47	40.36	-0.241	细胞质Cytoplasm
CmGolS11	MELO3C021249.2.1	331	38.29	4.78	42.58	-0.303	细胞质Cytoplasm
CmGolS12	MELO3C025521.2.1	325	37.79	5.60	40.54	-0.343	细胞质Cytoplasm

名称 Name	ID	氨基酸数 Animo acid number	分子质量/kD	等电点pI	不稳定系数Coefficient of instability	平均亲水指数 Grand average of hydropathicity	亚细胞定位 Prediction of subcellular localization
CmGolS1	MELO3C015870.2.1	367	42.13	8.70	41.22	-0.274	叶绿体Chloroplast
CmGolS2	MELO3C008407.2.1	655	76.19	6.64	45.07	-0.368	叶绿体Chloroplast
CmGolS3	MELO3C010862.2.1	520	61.73	8.18	53.33	-0.482	细胞质Cytoplasm
CmGolS4	MELO3C012925.2.1	335	38.51	4.97	44.32	-0.291	细胞质Cytoplasm
CmGolS5	MELO3C008614.2.1	414	46.95	6.53	40.70	-0.033	质膜Plasma membrane
CmGolS6	MELO3C004270.2.1	614	71.32	8.32	57.16	-0.332	叶绿体Chloroplast
CmGolS7	MELO3C008045.2.1	567	65.91	6.37	40.38	-0.351	细胞质Cytoplasm
CmGolS8	MELO3C022024.2.1	534	61.53	8.53	43.13	-0.179	液泡Vacuole
CmGolS9	MELO3C012034.2.1	377	42.76	6.86	52.29	-0.156	液泡Vacuole
CmGolS10	MELO3C011991.2.1	332	38.00	5.47	40.36	-0.241	细胞质Cytoplasm
CmGolS11	MELO3C021249.2.1	331	38.29	4.78	42.58	-0.303	细胞质Cytoplasm
CmGolS12	MELO3C025521.2.1	325	37.79	5.60	40.54	-0.343	细胞质Cytoplasm

Identification of Melon GolS Genes Family and the Expression Analysis in Response to Low Temperature Stress

RichHTML

PDF

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 8

References 41

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	TONG Zongjun, HAN Xing, DUAN Xinlian, LIU Yuanyuan, LIN Junbin, GAN Ying, CHEN Jie, XIE Baogui, GAN Bingcheng, and YAN Junjie, . Sequence Characterization of PRX Family and Expression Analysis in the Process of Fruiting Body Development in Flammulina filiformis [J]. Acta Horticulturae Sinica, 2025, 52(2): 337-348.
[2]	LIANG Guoping, ZENG Baozhen, LIU Ming, BIAN Zhiyuan, CHEN Baihong, MAO Juan. Identification of VaSR Gene Family in Vitis amurensis，Verification of Cold Resistance Function of VaSR1 and Screening of Interacting Proteins [J]. Acta Horticulturae Sinica, 2025, 52(1): 37-50.
[3]	GUO Changquan, LI Danqi, HUI Xinran, ZHENG Jingya, HOU Menglu, ZHU Yongxing. Identification and Expression Pattern Analysis of DUF966 Gene Family Members in Ginger [J]. Acta Horticulturae Sinica, 2024, 51(9): 2031-2047.
[4]	LIU Gangyun, DUAN Shixiang, XU Nana, GUO Yaomiao, DOU Junling, YANG Sen, NIU Huanhuan, LIU Dongming, YANG Luming, HU Jianbin, ZHU Huayu. Molecular Markers Assisted Construction of Cmerecta Near-Isogenic Line of Melon Dwarfing Gene [J]. Acta Horticulturae Sinica, 2024, 51(9): 2048-2062.
[5]	ZHAO Jiaying, ZENG Zhouting, CEN Xinying, SHI Jiaoqi, LI Xiaoxian, SHEN Xiaoxia, YU Zhenming. Genome-Wide Identification and Expression Analysis of CCO Gene Family in Dendrobium officinale During Flower Development [J]. Acta Horticulturae Sinica, 2024, 51(9): 2075-2088.
[6]	ZHANG Yong, MA Jianxiang, WEI Chunhua, LI Hao, YANG Jianqiang, ZHANG Xian. A New Muskmelon Cultivar‘Nongdatian 10’ [J]. Acta Horticulturae Sinica, 2024, 51(9): 2225-2226.
[7]	LI Ken, ZHANG Wei, WU Yunpeng, PENG Dongxiu, ZHANG Ruowei. Development and Utilization of KASP Marker for Identification of Pulp Firmness in Melon [J]. Acta Horticulturae Sinica, 2024, 51(4): 773-786.
[8]	LIU Yanyan, DING Ying, LIU Xinghua, ZHENG Jiaqiu, LIU Zhiqin. Identification of Pepper CaSYT1 and Its Function in Phytophthora capsici Infection [J]. Acta Horticulturae Sinica, 2024, 51(3): 533-544.
[9]	ZHANG Yongping, DIAO Qiannan, CAO Yanyan, LU Shijun, YAO Dongwei. A New Netted Melon Cultivar‘Lühua 18’ [J]. Acta Horticulturae Sinica, 2024, 51(3): 699-700.
[10]	YAN Chaofan, SUN Xuemei, ZHONG Qiwen, SHAO Dengkui, DENG Changrong, WEN Junqin. Identification and Bioinformatics Analysis of 20S Proteasome Gene Family in Tomato [J]. Acta Horticulturae Sinica, 2024, 51(2): 266-280.
[11]	LIU Liming, GENG Yanfei, HAO Fangmin, KANG Baoshan, WU Huijie, GU Qinsheng. Construction of Tomato Leaf Curl New Delhi Virus Infectious Clone and Its Application in Evaluation for Melon Disease Resistance [J]. Acta Horticulturae Sinica, 2024, 51(11): 2555-2564.
[12]	FENG Yiqing, QIU Shengnan, WU Yue, XIE Yang, ZHANG Xiaowei, BI Huangai, AI Xizhen. Effect of Exogenous Melatonin on the Cold Tolerance of Cucumber in Solar-Greenhouse [J]. Acta Horticulturae Sinica, 2024, 51(11): 2633-2644.
[13]	WU Qiong, ZHANG Tiantian, LI Maoying, WU Huiling, GUO Shaogui, ZHANG Jie, REN Yi, ZHANG Haiying, GONG Guoyi. Preliminary Study on the Prevention and Control Effect of Bacillus amyloliquefaciens Strain 5 on Cucumber Green Mottled Mosaic Virus Disease on Watermelon [J]. Acta Horticulturae Sinica, 2024, 51(10): 2427-2438.
[14]	HUANG Jinyan, HE Yi, LI Guifen, YE Yunfeng, XIE Huayun, LI Tianyan, HONG Rixin, QIN Sihua, LIU Tangjing, WEI Zhengguang, LI Zhi. A New Mid-Early Maturing Watermelon Cultivar‘Guixi 10’ [J]. Acta Horticulturae Sinica, 2024, 51(10): 2479-2480.
[15]	ZHANG Jie, GONG Guoyi, ZHANG Haiying, GUO Shaogui, REN Yi, LI Maoying, and XU Yong . A New Watermelon Hybrid‘Jingmei 10K03’ [J]. Acta Horticulturae Sinica, 2023, 50(S1): 101-102.