Preliminary Study on the Prevention and Control Effect of Bacillus amyloliquefaciens Strain 5 on Cucumber Green Mottled Mosaic Virus Disease on Watermelon

doi:10.16420/j.issn.0513-353x.2023-0798

Acta Horticulturae Sinica ›› 2024, Vol. 51 ›› Issue (10): 2427-2438.doi: 10.16420/j.issn.0513-353x.2023-0798

• Plant Protection • Previous Articles Next Articles

Preliminary Study on the Prevention and Control Effect of Bacillus amyloliquefaciens Strain 5 on Cucumber Green Mottled Mosaic Virus Disease on Watermelon

WU Qiong¹, ZHANG Tiantian², LI Maoying¹^,^*(), WU Huiling³, GUO Shaogui¹, ZHANG Jie¹, REN Yi¹, ZHANG Haiying¹, GONG Guoyi¹

¹ National Vegetable Engineering Technology Research Center，Vegetable Research Institute of Beijing Academy of Agriculture and Forestry，Beijing 100097，China
² School of Plant Science and Technology，Beijing Agricultural University，Beijing 102206，China
³ Institute of Plant Protection and Environmental Protection，Beijing Academy of Agricultural and Forestry Sciences，Beijing 100097，China

Received:2024-04-08 Revised:2024-07-19 Online:2024-10-25 Published:2024-10-21
Contact: LI Maoying

Abstract

Abstract:

In order to screen for effective microbial agents against cucumber green mottled mosaic virus（CGMMV）disease on watermelon，Paenibacillus polymyxa 246-1，Bacillus velezensis 4-2，and B. amyloliquefaciens 5 microbial agents were applied in advance on watermelon seedlings inoculated with CGMMV. The results showed that P. polymyxa 246-1，B. velezensis 4-2，and B. amyloliquefaciens 5 control effects of CGMMV disease reached 59.89%，46.71%，and 65.77%，respectively. Among them，B. amyloliquefaciens 5 had the best prevention and control effect. Further determination of physiological and biochemical indicators and phenotypic changes in watermelon seedlings on the 21st day was conducted to verify the prevention and control of CGMMV disease by pre-treatment with B. amyloliquefaciens. The results showed that the application of B. amyloliquefaciens 5 microbial agent in advance significantly increased the plant height，root length，fresh weight，and dry weight of watermelon seedlings by 5.93 cm，2.92 cm，2.48 g，and 2.09 g compared to the control group；the net photosynthetic rate，stomatal conductance，and transpiration rate of watermelon seedlings significantly increased by 351.00%，133.33%，and 91.30%，while the intercellular CO₂ concentration significantly decreased by 25.88%；simultaneously，the activities of SOD，POD，PAL，and CAT in watermelon seedlings were significantly increased by 100.37%，126.46%，98.45%，and 98.99%. In conclusion，B. amyloliquefaciens 5 can improve the resistance of watermelon to CGMMV by improving the growth and development of plants，the photosynthesis ability of leaves and the activities of resistant enzymes．

Key words: watermelon, cucumber green mottle mosaic virus, Bacillus amyloliquefaciens, biological prevention and control

WU Qiong, ZHANG Tiantian, LI Maoying, WU Huiling, GUO Shaogui, ZHANG Jie, REN Yi, ZHANG Haiying, GONG Guoyi. Preliminary Study on the Prevention and Control Effect of Bacillus amyloliquefaciens Strain 5 on Cucumber Green Mottled Mosaic Virus Disease on Watermelon[J]. Acta Horticulturae Sinica, 2024, 51(10): 2427-2438.

Figures/Tables 6

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菌剂 Microbial agent	发芽势/% Germinating energy	发芽率/% Germination percentage	根长/cm Root length	下胚轴长/cm Hypocotyledonary axis length
清水（对照） Water（control）	97.33 ± 0.94 a	100.00 ± 0.00 a	3.71 ± 0.06 c	3.39 ± 0.04 c
多粘芽孢杆菌246-1号 Paenibacillus polymyxa 246-1	95.67 ± 0.47 b	99.00 ± 0.82 b	3.78 ± 0.02 c	3.38 ± 0.02 c
贝莱斯芽孢杆菌4-2号 Bacillus velezensis 4-2	95.00 ± 1.41 b	99.33 ± 0.47 b	4.04 ± 0.01 b	3.62 ± 0.02 b
解淀粉芽孢杆菌5号 Bacillus amyloliquefaciens 5	98.33 ± 0.47 a	100.00 ±0.00 a	4.33 ± 0.00 a	3.80 ± 0.03 a

菌剂 Microbial agent	发芽势/% Germinating energy	发芽率/% Germination percentage	根长/cm Root length	下胚轴长/cm Hypocotyledonary axis length
清水（对照） Water（control）	97.33 ± 0.94 a	100.00 ± 0.00 a	3.71 ± 0.06 c	3.39 ± 0.04 c
多粘芽孢杆菌246-1号 Paenibacillus polymyxa 246-1	95.67 ± 0.47 b	99.00 ± 0.82 b	3.78 ± 0.02 c	3.38 ± 0.02 c
贝莱斯芽孢杆菌4-2号 Bacillus velezensis 4-2	95.00 ± 1.41 b	99.33 ± 0.47 b	4.04 ± 0.01 b	3.62 ± 0.02 b
解淀粉芽孢杆菌5号 Bacillus amyloliquefaciens 5	98.33 ± 0.47 a	100.00 ±0.00 a	4.33 ± 0.00 a	3.80 ± 0.03 a

菌剂 Microbial agent	发病率/% Rate of the disease	病情指数 Disease index	防控效果/% Prevention and control effects
单独接种CGMMV（对照） Inoculation with CGMMV individually（control）	100.00 ± 0.00 a	77.41 ± 0.85 a	—
多粘芽孢杆菌246-1 Paenibacillus polymyxa 246-1	71.63 ± 0.75 c	31.05 ± 0.59 c	59.89 ± 0.36 b
贝莱斯芽孢杆菌4-2 Bacillus velezensis 4-2	79.42 ± 0.67 b	33.75 ± 0.33 b	46.71 ± 0.42 c
解淀粉芽孢杆菌5 Bacillus amyloliquefaciens 5	67.50 ± 1.61 d	20.42 ± 0.83 d	65.77 ± 0.85 a

菌剂 Microbial agent	发病率/% Rate of the disease	病情指数 Disease index	防控效果/% Prevention and control effects
单独接种CGMMV（对照） Inoculation with CGMMV individually（control）	100.00 ± 0.00 a	77.41 ± 0.85 a	—
多粘芽孢杆菌246-1 Paenibacillus polymyxa 246-1	71.63 ± 0.75 c	31.05 ± 0.59 c	59.89 ± 0.36 b
贝莱斯芽孢杆菌4-2 Bacillus velezensis 4-2	79.42 ± 0.67 b	33.75 ± 0.33 b	46.71 ± 0.42 c
解淀粉芽孢杆菌5 Bacillus amyloliquefaciens 5	67.50 ± 1.61 d	20.42 ± 0.83 d	65.77 ± 0.85 a

Preliminary Study on the Prevention and Control Effect of Bacillus amyloliquefaciens Strain 5 on Cucumber Green Mottled Mosaic Virus Disease on Watermelon

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