Acta Horticulturae Sinica ›› 2024, Vol. 51 ›› Issue (9): 2048-2062.doi: 10.16420/j.issn.0513-353x.2023-0989
• Genetic & Breeding · Germplasm Resources · Molecular Biology • Previous Articles Next Articles
LIU Gangyun, DUAN Shixiang, XU Nana, GUO Yaomiao, DOU Junling, YANG Sen, NIU Huanhuan, LIU Dongming, YANG Luming, HU Jianbin, ZHU Huayu*()
Received:
2024-01-19
Revised:
2024-06-30
Online:
2024-09-25
Published:
2024-09-19
Contact:
ZHU Huayu
LIU Gangyun, DUAN Shixiang, XU Nana, GUO Yaomiao, DOU Junling, YANG Sen, NIU Huanhuan, LIU Dongming, YANG Luming, HU Jianbin, ZHU Huayu. Molecular Markers Assisted Construction of Cmerecta Near-Isogenic Line of Melon Dwarfing Gene[J]. Acta Horticulturae Sinica, 2024, 51(9): 2048-2062.
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URL: https://www.ahs.ac.cn/EN/10.16420/j.issn.0513-353x.2023-0989
Fig. 2 dCAPS2 distribution of foreground selection markers and 102 pairs of polymorphic SSR markers on the melon chromosome group The number on the left side represents the actual physical position of the background selection marker on the chromosome(Mb).
染色体 Chromosome | 多态性标记数量 Number of polymorphism markers | 平均距离/Mb Average distance | 标记间最大距离/Mb The maximum distance between markers |
---|---|---|---|
Chr1 | 7 | 5.5 | 17.6 |
Chr2 | 8 | 3.4 | 12.1 |
Chr3 | 9 | 3.0 | 10.0 |
Chr4 | 9 | 2.7 | 14.5 |
Chr5 | 11 | 2.5 | 8.7 |
Chr6 | 8 | 3.8 | 11.7 |
Chr7 | 9 | 3.0 | 12.3 |
Chr8 | 7 | 3.7 | 14.6 |
Chr9 | 9 | 2.9 | 13.5 |
Chr10 | 8 | 2.0 | 3.7 |
Chr11 | 7 | 4.2 | 10.5 |
Chr12 | 10 | 2.7 | 8.5 |
总计 Total | 102 | 3.1 | 11.5 |
Table 1 Distribution of background selection markers in melon near isogenic lines on the chromosome
染色体 Chromosome | 多态性标记数量 Number of polymorphism markers | 平均距离/Mb Average distance | 标记间最大距离/Mb The maximum distance between markers |
---|---|---|---|
Chr1 | 7 | 5.5 | 17.6 |
Chr2 | 8 | 3.4 | 12.1 |
Chr3 | 9 | 3.0 | 10.0 |
Chr4 | 9 | 2.7 | 14.5 |
Chr5 | 11 | 2.5 | 8.7 |
Chr6 | 8 | 3.8 | 11.7 |
Chr7 | 9 | 3.0 | 12.3 |
Chr8 | 7 | 3.7 | 14.6 |
Chr9 | 9 | 2.9 | 13.5 |
Chr10 | 8 | 2.0 | 3.7 |
Chr11 | 7 | 4.2 | 10.5 |
Chr12 | 10 | 2.7 | 8.5 |
总计 Total | 102 | 3.1 | 11.5 |
Fig. 3 BC2F1 population foreground selection and partial background selection results A:Digestion results of dCAPS2 in prospect selection. B:Amplification of background selection marker SSR07253.
Fig. 4 Graphrepresentation of candidate genotypes of individual strains of the BC2F1 population The red and blue regions of the genotype represent the genetic background of the reincarnation parent and the chromosome fragments introduced by the donor parent. Gray represents heterozygous fragment,and green represents the individual plant with the highest background recovery rate.
Fig. 5 Distribution of individual plant background selection markers across chromosomes with the highest BC2F1 background response rate The red and blue regions of the genotype represent the genetic background of the reincarnation parent and the chromosome fragments introduced by the donor parent. Gray represents heterozygous fragment.
Fig. 6 Graphrepresentation of candidate genotypes of individual strains of the BC2F2 population The red and blue regions of the genotype represent the genetic background of the reincarnation parent and the chromosome fragments introduced by the donor parent. Gray represents heterozygous fragment,and green represents the individual plant with the highest background recovery rate.
Fig. 7 Genotype distribution on the chromosome with the highest BC2F2 background response rate The red and blue regions of the genotype represent the genetic background of the reincarnation parent and the chromosome fragments introduced by the donor parent. Gray represents heterozygous fragment.
Fig. 8 Cmerecta-NIL phenotype observation(A,B)and trait statistics(C)at 30 days The statistical method of the histogram is ANOVA. The difference lowercase letters indicate significant differences at 0.05 level.
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