Investigation of the Causes of Seedlessness in‘Gongshui Baiyou’Pummelo

doi:10.16420/j.issn.0513-353x.2024-0246

Acta Horticulturae Sinica ›› 2025, Vol. 52 ›› Issue (1): 1-12.doi: 10.16420/j.issn.0513-353x.2024-0246

Investigation of the Causes of Seedlessness in‘Gongshui Baiyou’Pummelo

HU Yu¹, CAO Zonghong¹, SUN Yi¹, LIU Cong¹, WEN Hao¹, LIU Huimin¹, ZHENG Xin¹, XU Mengmeng¹, JIANG Xiangdong³, ZHANG Youhe³, LI Guanghao³, YE Junli¹, DENG Xiuxin¹^,², XIE Zongzhou¹^,^*(), CHAI Lijun¹^,²^,^*()

¹ National Key Laboratory of Germplasm Innovation and Utilization of Fruit and Vegetable Horticulture，College of Horticulture and Forestry，Huazhong Agricultural University，Wuhan 430070，China
² Hubei Hongshan Laboratory，Wuhan 430070，China
³ Agricultural Technology Service Center of Gaoluo Town/Agricultural Technology Service Center of Lijiahe Town/Agricultural Technology Service Center of Shadaogou Town，Xuan’en County，Enshi，Hubei 445000，China

Received:2024-07-03 Revised:2024-11-25 Online:2025-01-25 Published:2025-01-18

Abstract

Abstract:

The seedlessness cause of‘Gongshui Baiyou’pummelo was investigated through morphological and cytological observations. The results showed that both the female and male gametophytes of‘Gongshui Baiyou’developed normally and were fertile. After self-pollination，pollen tube growth stopped at 1/3 of the stigma，showing incompatibility. The fruit setting rates of unpollinated flowers after 7，14，21 and 49 days were 60%，32%，14% and 9%，respectively，and they could develop into seedless fruits，which indicated that they had parthenocarpic ability. Additionally，the sectioning of ovule tissue development in the early stage showed that the embryo sac tissue of seedless‘Gongshui Baiyou’began to shrink 21 days after emasculation. Its cross with‘Shatianyou’and‘Gongshui Baiyou’pummelos showed affinity and could contain full and normal seeds. In conlution，both self-incompatibility and parthenocarpy are the main reasons for the formation of seedless fruit of‘Gongshui Baiyou’.

Key words: pummelo, seedlessness, parthenocarpy, self-incompatibility

HU Yu, CAO Zonghong, SUN Yi, LIU Cong, WEN Hao, LIU Huimin, ZHENG Xin, XU Mengmeng, JIANG Xiangdong, ZHANG Youhe, LI Guanghao, YE Junli, DENG Xiuxin, XIE Zongzhou, CHAI Lijun. Investigation of the Causes of Seedlessness in‘Gongshui Baiyou’Pummelo[J]. Acta Horticulturae Sinica, 2025, 52(1): 1-12.

Figures/Tables 7

References 29

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试验材料 Plant material	单果质量/g Fruit weight	纵径/mm Vertical diameter	横径/mm Transverse diameter	皮厚/mm Thick skin	可溶性固形物/% Total soluble solids	可滴定酸/% Titratable acidity	固酸比 TSS/acidity ratio	维生素C/ （mg · kg^-1） Vitamin C
贡水白柚 Gongshui Baiyou	1 158.0 ± 178.2 b	155.5 ± 15.2 b	183.2 ± 11.5 a	28.3 ± 1.8 a	12.1 ± 0.4 c	0.6 ± 0.1 a	18.9 ± 1.4 b	545.0 ± 2.4 a
广安柚 Guanganyou	1 007.7 ± 137.6 c	132.3 ± 5.8 c	158.9 ± 12.2 bc	18.6 ± 1.1 c	11.0 ± 0.2 de	0.6 ± 0.1 a	17.7 ± 2.9 c	574.0 ± 4.3 a
红肉蜜柚 Hongrou Miyou	1 189.6 ± 87.8 bc	138.8 ± 5.2 bc	154.1 ± 16.6 bc	13.8 ± 1.3 d	14.5 ± 0.5 a	0.6 ± 0.04 ab	22.8 ± 1.6 b	421.0 ± 5.0 c
水晶柚 Shuijingyou	919.4 ± 50.8 c	138.2 ± 0.6 bc	147.3 ± 5.0 c	19.4 ± 0.5 c	14.9 ± 0.2 a	0.5 ± 0.1 b	32.5 ± 7.74 a	343.0 ± 0.4 d
玉环柚 Yuhuanyou	2 015.6 ± 267.3 a	184.5 ± 4.5 a	180.6 ± 13.9 a	24.3 ± 2.8 b	10.2 ± 0.2 e	0.4 ± 0.1 c	29.0 ± 10.0 ab	423.0 ± 1.2 c
黄金蜜柚 Huangjin Miyou	1 352.6 ± 40.9 b	152.5 ± 3.2 b	165.6 ± 9.8 b	13.2 ± 2.8 d	13.1 ± 0.6 b	0.4 ± 0.1 c	32.5 ± 5.9 a	446.0 ± 4.4 bc
琯溪蜜柚 Guanxi Miyou	1 093.3 ± 217.6 bc	147.4 ± 2.6 bc	163.8 ± 9.4 b	21.6 ± 2.7 b	13.4 ± 1.0 b	0.6 ± 0.2 ab	21.8 ± 4.1 bc	426.0 ± 1.0 c
西施柚 Xishiyou	1 025.4 ± 89.6 c	143.2 ± 5.6 bc	169.5 ± 7.5 b	17.4 ± 1.1 c	11.5 ± 0.8 cd	0.6 ± 0.2 ab	21.8 ± 8.2 bc	487.0 ± 2.8 b

试验材料 Plant material	单果质量/g Fruit weight	纵径/mm Vertical diameter	横径/mm Transverse diameter	皮厚/mm Thick skin	可溶性固形物/% Total soluble solids	可滴定酸/% Titratable acidity	固酸比 TSS/acidity ratio	维生素C/ （mg · kg^-1） Vitamin C
贡水白柚 Gongshui Baiyou	1 158.0 ± 178.2 b	155.5 ± 15.2 b	183.2 ± 11.5 a	28.3 ± 1.8 a	12.1 ± 0.4 c	0.6 ± 0.1 a	18.9 ± 1.4 b	545.0 ± 2.4 a
广安柚 Guanganyou	1 007.7 ± 137.6 c	132.3 ± 5.8 c	158.9 ± 12.2 bc	18.6 ± 1.1 c	11.0 ± 0.2 de	0.6 ± 0.1 a	17.7 ± 2.9 c	574.0 ± 4.3 a
红肉蜜柚 Hongrou Miyou	1 189.6 ± 87.8 bc	138.8 ± 5.2 bc	154.1 ± 16.6 bc	13.8 ± 1.3 d	14.5 ± 0.5 a	0.6 ± 0.04 ab	22.8 ± 1.6 b	421.0 ± 5.0 c
水晶柚 Shuijingyou	919.4 ± 50.8 c	138.2 ± 0.6 bc	147.3 ± 5.0 c	19.4 ± 0.5 c	14.9 ± 0.2 a	0.5 ± 0.1 b	32.5 ± 7.74 a	343.0 ± 0.4 d
玉环柚 Yuhuanyou	2 015.6 ± 267.3 a	184.5 ± 4.5 a	180.6 ± 13.9 a	24.3 ± 2.8 b	10.2 ± 0.2 e	0.4 ± 0.1 c	29.0 ± 10.0 ab	423.0 ± 1.2 c
黄金蜜柚 Huangjin Miyou	1 352.6 ± 40.9 b	152.5 ± 3.2 b	165.6 ± 9.8 b	13.2 ± 2.8 d	13.1 ± 0.6 b	0.4 ± 0.1 c	32.5 ± 5.9 a	446.0 ± 4.4 bc
琯溪蜜柚 Guanxi Miyou	1 093.3 ± 217.6 bc	147.4 ± 2.6 bc	163.8 ± 9.4 b	21.6 ± 2.7 b	13.4 ± 1.0 b	0.6 ± 0.2 ab	21.8 ± 4.1 bc	426.0 ± 1.0 c
西施柚 Xishiyou	1 025.4 ± 89.6 c	143.2 ± 5.6 bc	169.5 ± 7.5 b	17.4 ± 1.1 c	11.5 ± 0.8 cd	0.6 ± 0.2 ab	21.8 ± 8.2 bc	487.0 ± 2.8 b

Investigation of the Causes of Seedlessness in‘Gongshui Baiyou’Pummelo

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