Promoter of Vegetable Soybean GmDi19-3 Responds to Salt Stress and Exogenous ABA and MeJA

doi:10.16420/j.issn.0513-353x.2023-0934

Acta Horticulturae Sinica ›› 2024, Vol. 51 ›› Issue (12): 2791-2799.doi: 10.16420/j.issn.0513-353x.2023-0934

• Genetic & Breeding·Germplasm Resources·Molecular Biology • Previous Articles Next Articles

Promoter of Vegetable Soybean GmDi19-3 Responds to Salt Stress and Exogenous ABA and MeJA

FENG Zhijuan, LIU Na, ZHANG Guwen, BU Yuanpeng, WANG Bin, GONG Yaming()

Key Laboratory of Vegetable Legumes Germplasm Enhancement and Molecular Breeding in Southern China of Ministry of Agriculture and Rural Affairs，State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Vegetables，Zhejiang Academy of Agricultural Sciences，Hangzhou 310021，China

Received:2024-10-08 Revised:2024-11-13 Online:2024-12-25 Published:2024-12-13
Contact: GONG Yaming

Abstract

Abstract:

To clarify the regulation function of vegetable soybean GmDi19-3 promoter，the expression patterns of GmDi19-3 gene in different tissues and in response to salt stress and exogenous plant growth regulators were analyzed. The promoter of GmDi19-3 was cloned and cis-acting element composition was analyzed. The promoter of GmDi19-3 was fused with the GUS gene in the expression vector which was stably expressed in Arabidopsis. The promoter activity of GmDi19-3 was detected in the transgenic plants. Typical salt stress and exogenous ABA and MeJA signal inducible expression patterns of GmDi19-3 were observed. The promoter of GmDi19-3 harbored abiotic stress and hormone signal responsive elements. Based on the GUS staining and activity detection assays，it was verified that salt stress and exogenous ABA and MeJA signals obviously enhanced the promoter activity of GmDi19-3 in the leaf，stem and root of transgenic plants. These results suggested that GmDi19-3 promoter played key roles in response to salt stress，and exogenous ABA and MeJA signals.

Key words: vegetable soybean, Di19-3, promoter, GUS activity, salt stress, ABA, MeJA

FENG Zhijuan, LIU Na, ZHANG Guwen, BU Yuanpeng, WANG Bin, GONG Yaming. Promoter of Vegetable Soybean GmDi19-3 Responds to Salt Stress and Exogenous ABA and MeJA[J]. Acta Horticulturae Sinica, 2024, 51(12): 2791-2799.

Figures/Tables 7

Table 1 Distribution of cis-acting elements in the GmDi19-3 promoter of vegetable soybean

元件名称 Element name	核心序列 Core sequence	数量 Number	位置/bp Location		功能 Function
元件名称 Element name	核心序列 Core sequence	数量 Number	（+）链（+）Strand	（-）链（-）Strand	功能 Function
ABRE	ACGTG	2	452 757		脱落酸响应ABA responsiveness
ARE	AAACCA	2	498 958		厌氧诱导 Anaerobic induction
CGTCA基序 GTCA-motif	CGTCA	1		755	茉莉酸响应MeJA responsiveness
ERE	ATTTTAAA	1		236	乙烯响应ET responsiveness
LTR	CCGAAA	1		947	低温响应Low temperature responsiveness
MYB	CAACCA	2	502	312	激素信号诱导和非生物胁迫响应 Hormone signal induction andabiotic stress responsiveness
MYB识别位点 MYB recognition site	CCGTTG	1	605
MYC	GTTTAC	1		133	植物生长和防御响应Plant growth and defense responsiveness
TCA元件 TCA-element	CCATCTTTTT	1		831	水杨酸响应SA responsiveness
TGA元件 TGA-element	AACGAC	1		485	生长素响应Auxin responsiveness
TGACG基序 TGACG-motif	TGACG	1		191	茉莉酸响应MeJA responsiveness

Fig. 1 Expression patterns of GmDi19-3 in different tissues of vegetable soybean **：Significant differences in comparison between the leaf and stem，root and stem（t-test，P < 0.01）.

Fig. 2 GUS staining of GmDi19-3 promoter in young seedling, primary and lateral roots of transgenic Arabidopsis

Fig. 3 Expression patterns of GmDi19-3 in vegetable soybean after salt，exogenous ABA and MeJA treatments **：Significant differences in comparison between the treatment and control seedlings（t-test，P < 0.01）. The same below.

Fig. 4 GUS staining and activity of proGmDi19-3::GUS promoter in transgenic Arabidopsis seedlings after salt treatment

Fig. 5 GUS staining and activity of proGmDi19-3::GUS promoter in transgenic Arabidopsis seedlings after exogenous ABA treatment

Fig. 6 GUS staining and activity of proGmDi19-3::GUS promoter in transgenic Arabidopsis seedlings after exogenous MeJA treatment

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Promoter of Vegetable Soybean GmDi19-3 Responds to Salt Stress and Exogenous ABA and MeJA

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