Storage with Climacteric Fruits Improves Color and Quality of Citrus Fruit

doi:10.16420/j.issn.0513-353x.2022-1203

Acta Horticulturae Sinica ›› 2024, Vol. 51 ›› Issue (3): 601-615.doi: 10.16420/j.issn.0513-353x.2022-1203

• Cultivation·Physiology & Biochemistry • Previous Articles Next Articles

Storage with Climacteric Fruits Improves Color and Quality of Citrus Fruit

SUN Quan, HE Zhengchen, YE Junli, WEI Ranran, YIN Yingzi, CHAI Lijun, XIE Zongzhou, XU Qiang, XU Juan, GUO Wenwu, CHENG Yunjiang, DENG Xiuxin^**()

Key Laboratory of Horticultural Plant Biology，Ministry of Education，National Citrus Breeding Centre，College of Horticulture & Forestry Science，Huazhong Agricultural University，Wuhan 430070，China

Received:2023-09-25 Revised:2023-11-13 Online:2024-03-25 Published:2024-03-22
Contact: DENG Xiuxin

Abstract

Abstract:

Nine common horticultural fruits were selected for postharvest co-storage with citrus fruits，respectively. The results showed that storage with climacteric fruits（fragrant pear，kiwifruit，tomato，and banana）significantly promoting the citrus peel redden，especially storage with fragrant pear. High performance liquid chromatography（HPLC）analysis revealed that storage with climacteric fruits（SWCF）stimulated an approximately 3-fold increase in the content of total carotenoid，β-citraurin，β-cryptoxanthin，and violaxanthin. Especially，the accumulation of β-citraurin mainly responsible for SWCF-induced citrus peel redden. Real-time fluorescent quantitative PCR（qRT-PCR）results showed that SWCF dramatically activated the expressions of carotenogenic genes，among which，carotenoid cleavage dioxygenase 4b（CsCCD4b）transcript was enhanced about 20 folds，thereby directly resulting in β-citraurin accumulation. Furthermore，SWCF treatment has wide applicability in promoting citrus peel coloration. In addition，SWCF obviously maintained the storage performances of citrus fruit by alleviating the calyx senescence，weight loss，and wax degradation than ethylene treatment. Moreover，the contents of volatile substances in SWCF-treated fruit were higher than control and ethylene treatments. SWCF provides a new idea for green and efficient improvement of citrus fruit quality.

Key words: citrus, climacteric fruits, biological treatment, peel coloration, fruit quality, postharvest storage treatment

SUN Quan, HE Zhengchen, YE Junli, WEI Ranran, YIN Yingzi, CHAI Lijun, XIE Zongzhou, XU Qiang, XU Juan, GUO Wenwu, CHENG Yunjiang, DENG Xiuxin. Storage with Climacteric Fruits Improves Color and Quality of Citrus Fruit[J]. Acta Horticulturae Sinica, 2024, 51(3): 601-615.

Figures/Tables 10

References 40

[1]	Ali A, Maqbool M, Ramachandran S, Alderson P G. 2010. Gum arabic as a novel edible coating for enhancing shelf-life and improving postharvest quality of tomato(Solanum lycopersicum L.)fruit. Postharvest Biology and Technology,58:42-47.
[2]	Alvillo-Rivera A, Garrido-Hoyos S, Buitrón G, Thangarasu-Sarasvathi P, Rosano-Ortega G. 2021. Biological treatment for the degradation of cyanide:a review. Journal of Materials Research and Technology,12:1418-1433.
[3]	Bai J, Alleyne V, Hagenmaier R D, Mattheis J P, Baldwin E A. 2003. Formulation of zein coatings for apples(Malus domestica Borkh.). Postharvest Biology and Technology,28:259-268.
[4]	Brown G E, Burns J K. 1998. Enhanced activity of abscission enzymes predisposes oranges to invasion by Diplodia natalensis during ethylene degreening. Postharvest Biology and Technology,14:217-227.
[5]	Carvalho C P, Salvador A, Navarro P, Monterde A, Martínez-Jávega J M. 2008. Effect of auxin treatments on calyx senescence in the degreening of four mandarin cultivars. J Am Soc Horticultural Science,43:747-752.
[6]	Deng Xiuxin. 2022. A review and perspective for citrus breeding in China during the last six decades. Acta Horticulturase Sinica, 49 (10):2063-2074. (in Chinese)
	邓秀新. 2022. 中国柑橘育种60年回顾与展望. 园艺学报, 49 (10):2063-2074. doi: 10.16420/j.issn.0513-353x.2021-0701
[7]	Deng Xiu-xin, Peng Shu-ang. 2013. Citrus science. Beijing: China Agriculture Press. (in Chinses)
	邓秀新, 彭抒昂. 2013. 柑橘学. 北京: 中国农业出版社.
[8]	Fraser P D, Bramley P M. 2003. The biosynthesis and nutritional uses of carotenoids. Progress in Lipid Research, 43 (3):228-265.
[9]	Gapper N E, McQuinn R P, Giovannoni J J. 2013. Molecular and genetic regulation of fruit ripening. Plant Mol Biol,82:575-591.
[10]	Huang Yong-hong, Li Chun-yu, Zuo Cun-wu, Mei Mei, Yi Gan-jun. 2011. Suppressive effects of Chinese leek(Allium tuberosum)on Fusarium wilt disease of Baxi banana. Chinese Journal of Biological Control, 27 (3):344-348. (in Chinese)
	黄永红, 李春雨, 左存武, 梅眉, 易干军. 2011. 韭菜对巴西香蕉枯萎病发生的抑制作用. 中国生物防治学报, 27 (3):344-348.
[11]	Ikoma Y, Matsumoto H, Kato M. 2016. Diversity in the carotenoid profiles and the expression of genes related to carotenoid accumulation among citrus genotypes. Breeding Science,66:139-147.
[12]	Jiang Q H, Ye J L, Zhu K J, Wu F F, Chai L J, Xu Q, Deng X X. 2022. Transcriptome and co-expression network analyses provide insights into fruit shading that enhances carotenoid accumulation in pomelo(Citrus grandis). Horticultural Plant Journal, 8 (4):423-434.
[13]	Kato M, Ikoma Y, Matsumoto H, Sugiura M, Hyodo H, Yano M. 2004. Accumulation of carotenoids and expression of carotenoid biosynthetic genes during maturation in citrus fruit. Plant Physiology,134:824-837.
[14]	Koch K, Ensikat H J. 2008. The hydrophobic coatings of plant surfaces:epicuticular wax crystals and their morphologies,crystallinity and molecular self-assembly. Micron, 39 (7):759-772.
[15]	Li F, Min D, Ren C, Dong L, Shu P, Cui X, Zhang X. 2019. Ethylene altered fruit cuticular wax,the expression of cuticular wax synthesis-related genes and fruit. Postharvest Biology and Technology,149:58-65.
[16]	Li Hai. 2005. Mechanism of increasing yield of intercropping and mixed cropping patterns of Alfalfa and Gramineous crops[M. D. Dissertation]. Hohhot: Inner Mongolia Agricultural University. (in Chinese)
	李海. 2005. 苜蓿与禾本科作物间混作增产机制[硕士论文]. 呼和浩特: 内蒙古农业大学.
[17]	Li T, Li X, Tan D, Jiang Z, Wei Y, Li J, Du G, Wang A. 2014. Distinct expression profiles of ripening related genes in the‘Nanguo’pear(Pyrus ussuriensis)fruits. Scientia Horticulturae,171:78-82.
[18]	Liu G, Chen Y, He X, Yao F, Guan G, Zhong B, Zhou G. 2020. Seasonal changes of mineral nutrients in the fruit of navel orange plants grafted on trifoliate orange and citrange. Scientia Horticulturae,264:109156.
[19]	Liu H M, Guo J H, Cheng Y J, Luo L, Liu P, Wang B Q, Deng B X, Long C A. 2010. Control of gray mold of grape by Hanseniaspora uvarum and its effects on postharvest quality parameters. Ann Microbiology,60:31-35.
[20]	Lu Chenfei, Gao Yuexia, Huang He, Dai Silan. 2022. Carotenoid metabolism and regulation in plants. Acta Horticulturae Sinica, 49 (12):2559-2578. (in Chinese) doi: 10.16420/j.issn.0513-353x.2021-0531
	陆晨飞, 高月霞, 黄河, 戴思兰. 2022. 植物类胡萝卜素代谢及调控研究进展. 园艺学报, 49 (12):2559-2578. doi: 10.16420/j.issn.0513-353x.2021-0531
[21]	Lu S, Zhang Y, Zhu K, Yang W, Ye J, Chai L, Xu Q, Deng X. 2018. The citrus transcription factor CsMADS6 modulates carotenoid metabolism by directly regulating carotenogenic genes. Plant Physiology,176:2657.
[22]	Ma G, Zhang L, Kato M, Yamawaki K, Kiriiwa Y, Yahata M, Ikoma Y, Matsumoto H. 2012. Effect of blue and red led light irradiation on β-cryptoxanthin accumulation in the flavedo of citrus fruits. Journal of Agricultural and Food Chemistry,60:197-201.
[23]	Martínez-Romero D, Alburquerque N, Valverde J M, Guillén F, Castillo S, Valero D, Serrano M. 2006. Postharvest sweet cherry quality and safety maintenance by aloe vera treatment:a new edible coating. Postharvest Biology and Technology,39:93-100.
[24]	Matsumoto H, Ikoma Y, Kato M, Kuniga T, Nakajima N, Yoshida T. 2007. Quantification of carotenoids in citrus fruit by LC-MS and comparison of patterns of seasonal changes for carotenoids among citrus varieties. Journal of Agricultural and Food Chemistry,55:2356-2368.
[25]	Matsumoto H, Ikoma Y, Kato M, Nakajima N, Hasegawa Y. 2009. Effect of postharvest temperature and ethylene on carotenoid accumulation in the flavedo and juice sacs of satsuma mandarin(Citrus unshiu Marc.) fruit. Journal of Agricultural and Food Chemistry,57:4724-4732.
[26]	Mayuoni L, Tietel Z, Patil B S, Porat R. 2011. Does ethylene degreening affect internal quality of citrus fruit? Postharvest Biol. Technol,62:50-58.
[27]	Meng X, Li B, Liu J, Tian S. 2008. Physiological responses and quality attributes of table grape fruit to chitosan preharvest spray and postharvest coating during storage. Food Chemistry,106:501-508.
[28]	Nisar N, Li L, Lu S, Khin N C, Pogson B J. 2015. Carotenoid metabolism in plants. Molecular Plant, 8 (1):68-82. doi: 10.1016/j.molp.2014.12.007 pmid: 25578273
[29]	Rehman M, Singh Z, Khurshid T. 2018. Pre-harvest spray application of abscisic acid(S-ABA)regulates fruit colour development and quality in early maturing M7 navel orange. Scientia Horticulturae,229:1-9.
[30]	Rodrigo M J, Alquézar B, Alós E, Lado J, Zacarías L. 2013. Biochemical bases and molecular regulation of pigmentation in the peel of citrus fruit. Scientia Horticulturae,163:46-62.
[31]	Rodrigo M J, Zacarias L. 2007. Effect of postharvest ethylene treatment on carotenoid accumulation and the expression of carotenoid biosynthetic genes in the flavedo of orange(Citrus sinensis L. Osbeck)fruit. Postharvest Biology and Technology, 43 (1):14-22.
[32]	Sun Q, He Y, Ye J, Zheng X, Zhou C, Fu A, Wei R, Yin Y, Chai L, Xu Q, Cheng Y, Deng X. 2021. Storage with apple fruit to improve peel color and maintain freshness of‘Newhall’navel orange. Scientia Horticulturae,287:110246.
[33]	Sun Quan. 2022. Research on the mechanism of biotechnology treatment improving citrus peel color and ethylene regulating carotenoid metabolism in citrus[Ph. D. Dissertation]. Wuhan: Huazhong Agricultural University. (in Chinese)
	孙权. 2022. 生物处理改善柑橘果皮色泽及乙烯调控柑橘类胡萝卜素代谢的机制研究[博士论文]. 武汉: 华中农业大学.
[34]	Wang J, Hao H, Liu R, Ma Q, Xu J, Chen F, Cheng Y, Deng X. 2014. Comparative analysis of surface wax in mature fruits between Satsuma mandarin(Citrus unshiu)and‘Newhall’navel orange(Citrus sinensis)from the perspective of crystal morphology,chemical composition and key gene expression. Food Chemistry,153:177-185.
[35]	Wang J M, Zhao M Y, Gao T, Feng Y Y, Wang F Q, PanY T, JinJ Y, JingT T, Lu M Q, Zhang M T, Guo D Y, WanX C, Schwab W, Song C K. 2023. Promoter and coding sequence diversity of CsCCD1may contribute to the differential accumulation of floral β‐ionone in fresh tea leaves. Horticultural Plant Journal, 9 (4):837-847.
[36]	Yuan H, Zhang J, Nageswaran D, Li L. 2015. Carotenoid metabolism and regulation in horticultural crops. Horticulture Research,(2):15036.
[37]	Zhang H, Xie Y, Liu C, Chen S, Hu S, Xie Z, Deng X, Xu J. 2017. Comprehensive comparative analysis of volatile compounds in citrus fruits of different species. Food Chemistry,230:316-326.
[38]	Zheng Tianyi, Hu Yujie, Zhang Xuehao, Li Wanping, Fang Yulin, Zhang Kekun, Chen Keqin. 2023. Advances in the regulation of anthocyanin biosynthesis by hormone signaling in fruit trees. Acta Horticulturae Sinica, 50 (11):2516-2536. (in Chinese) doi: 10.16420/j.issn.0513-353x.2022-1038
	郑天一, 胡玉杰, 张学昊, 李婉平, 房玉林, 张克坤, 陈可钦. 2023. 激素信号调控果树果实花色苷合成机制研究进展. 园艺学报, 50 (11):2516-2536. doi: 10.16420/j.issn.0513-353x.2022-1038
[39]	Zheng X, Zhu K, Sun Q, Zhang W, Wang X, Cao H, Tan M, Xie Z, Zeng Y, Ye J, Chai L, Xu Q, Pan Z, Xiao S, Fraser P D, Deng X. 2019. Natural variation in CCD4 promoter underpins species-specific evolution of red coloration in citrus peel. Molecular Plant.,12:1294-1307.
[40]	Zhou J Y, Sun C D, Zhang L L, Dai X, Xu C J, Chen K S. 2010. Preferential accumulation of orange-colored carotenoids in ponkan(Citrus reticulata)fruit peel following postharvest application of ethylene or ethephon. Scientia Horticulturae,126:229-235.

类型 Type	物种 Species	品种 Cultivar	乙烯释放速率/ （μg · kg^-1 · h^-1）	来源地 Origin
呼吸跃变型 Climacteric	香梨 Korla pear	库尔勒香梨 Korla	18.25	新疆 Xinjiang
	猕猴桃 Kiwifruit	翠香猕猴桃Cuixiang	70.05	陕西 Shaanxi
	番茄Tomato	奥美拉番茄 Omega	18.16	湖北 Hubei
	香蕉 Banana	巴西蕉 Cavendish	8.85	海南 Hainan
非呼吸跃变型 Non-climacteric	鸭梨 Yali pear	冠县鸭梨 Guanxian Yali	2.42	山东 Shandong
	草莓 Strawberry	宁玉草莓Ningyu	1.32	江苏 Jiangsu
	枣 Jujube	阜平大枣Fuping Dazao	1.55	河北 Hebei
	葡萄 Grape	巨峰葡萄 Kyoho	0.87	山东 Shandong
	枇杷 Loquat	白玉枇杷 Baiyu	2.34	江苏 Jiangsu

类型 Type	物种 Species	品种 Cultivar	乙烯释放速率/ （μg · kg^-1 · h^-1）	来源地 Origin
呼吸跃变型 Climacteric	香梨 Korla pear	库尔勒香梨 Korla	18.25	新疆 Xinjiang
	猕猴桃 Kiwifruit	翠香猕猴桃Cuixiang	70.05	陕西 Shaanxi
	番茄Tomato	奥美拉番茄 Omega	18.16	湖北 Hubei
	香蕉 Banana	巴西蕉 Cavendish	8.85	海南 Hainan
非呼吸跃变型 Non-climacteric	鸭梨 Yali pear	冠县鸭梨 Guanxian Yali	2.42	山东 Shandong
	草莓 Strawberry	宁玉草莓Ningyu	1.32	江苏 Jiangsu
	枣 Jujube	阜平大枣Fuping Dazao	1.55	河北 Hebei
	葡萄 Grape	巨峰葡萄 Kyoho	0.87	山东 Shandong
	枇杷 Loquat	白玉枇杷 Baiyu	2.34	江苏 Jiangsu

用途 Usage	基因名 Gene name	正向引物序列（5′-3′） Forward primer sequence	反向引物序列（5′-3′） Reverse primer sequence
内参 Reference gene	ACTIN	CCAAGCAGCATGAAGATCAA	ATCTGCTGGAAGGTGCTGAG
类胡萝卜素代谢 Carotenoid metabolism	CsPSY	CCCGGACTGCTGTGTTTAAT	GAGCAAGGATGCCTCAAATC
	CsPDS	ATAATTGGCGGACAGGCATA	CCTCTGTCGTCACTCGATCA
	CsHYD	TTTGGGATGGCCTACATGTTC	GGCACGTCGGCAATGG
	CsZEP	GAAGCAATTCTTCGACGTGACA	ACCGAGTCCCCAAGCAAAGT
	CsCCD4b	CAGCAAGAAATTTGGAGTTG	CGTAAAATCTTCTTGAGAC
	CsNCED2	GCCCAACAAATCCTTGAAAGTAGA	GGGTAAGGCTGTTTAGGGAAATG
蜡质代谢 Wax metabolism	CsCER1	TCGCTATTTATGCCAATTTATG	TGATTCTTCTTCTTCTTCACCTGAT
	CsCER2	GGCCCTGGAGTCGTGACA	CGATGCCGTCTCGTTTTCTC
	CsCER3	CAAGCAGCTCAACATTCCAA	ATCGTCAGGGAATCTCATGG
	CsCER4	TTGATATGGTGGTGAATGCTATGA	TTGATATGGTGGTGAATGCTATGA

用途 Usage	基因名 Gene name	正向引物序列（5′-3′） Forward primer sequence	反向引物序列（5′-3′） Reverse primer sequence
内参 Reference gene	ACTIN	CCAAGCAGCATGAAGATCAA	ATCTGCTGGAAGGTGCTGAG
类胡萝卜素代谢 Carotenoid metabolism	CsPSY	CCCGGACTGCTGTGTTTAAT	GAGCAAGGATGCCTCAAATC
	CsPDS	ATAATTGGCGGACAGGCATA	CCTCTGTCGTCACTCGATCA
	CsHYD	TTTGGGATGGCCTACATGTTC	GGCACGTCGGCAATGG
	CsZEP	GAAGCAATTCTTCGACGTGACA	ACCGAGTCCCCAAGCAAAGT
	CsCCD4b	CAGCAAGAAATTTGGAGTTG	CGTAAAATCTTCTTGAGAC
	CsNCED2	GCCCAACAAATCCTTGAAAGTAGA	GGGTAAGGCTGTTTAGGGAAATG
蜡质代谢 Wax metabolism	CsCER1	TCGCTATTTATGCCAATTTATG	TGATTCTTCTTCTTCTTCACCTGAT
	CsCER2	GGCCCTGGAGTCGTGACA	CGATGCCGTCTCGTTTTCTC
	CsCER3	CAAGCAGCTCAACATTCCAA	ATCGTCAGGGAATCTCATGG
	CsCER4	TTGATATGGTGGTGAATGCTATGA	TTGATATGGTGGTGAATGCTATGA

Storage with Climacteric Fruits Improves Color and Quality of Citrus Fruit

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