Evaluation of Fruit Segmental Membrane Development and Mastication in Two Citrus Triploids

doi:10.16420/j.issn.0513-353x.2023-0493

Acta Horticulturae Sinica ›› 2024, Vol. 51 ›› Issue (6): 1179-1188.doi: 10.16420/j.issn.0513-353x.2023-0493

• Genetic & Breeding·Germplasm Resources·Molecular Biology • Next Articles

Evaluation of Fruit Segmental Membrane Development and Mastication in Two Citrus Triploids

CAO Huixiang¹^,², LUO Xin¹, LIU Wanrong¹, GUAN Shuping¹, WANG Tingting¹, WU Xiaomeng¹, GUO Wenwu¹, XIE Kaidong¹^,^*()

¹ National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops，College of Horticulture & Forestry Sciences，Huazhong Agricultural University，Wuhan 430070，China
² Xiangyang Academy of Agricultural Science，Xiangyang，Hubei 441100，China

Received:2024-02-04 Revised:2024-04-24 Online:2024-12-18 Published:2024-06-21
Contact: XIE Kaidong

Abstract

Abstract:

To investigate the reason for the fruit mastication difference between citrus triploid progeny with their diploid parents，two triploid hybrids derived from the crosses with Bendizao tangerine and Red tangerine as female parent were selected as materials to investigate the effect of segmental membrane structure and its components on the mastication of triploid fruits comparing with their diploid parents. In this study，using the two diploid parents as the control，the fruit mastication of the two triploid hybrids was firstly determined with texture analyzer at mature stage. It showed that the hardness and shear force of the segmental membrane of the two triploid progenies were significantly higher than that of their diploid parents，indicating that the segmental membranes of the two triploid hybrids might thicker than that of their corresponding diploid parents. It showed that the thickness and cell layer number of the segmental membranes of the two triploid hybrids were significantly higher than that of their corresponding diploid parents at the same developmental stage started from 120 days after flowering（DAF）. The ultrastructure of the segmental membrane showed that the cell walls in the two triploid hybrids were thicker and the cellulose microfibrils were denser than those of their diploid parents. The content of water-soluble pectin，protopectin，cellulose and hemicellulose in the segment membrane of the two triploid hybrids were significantly higher than that of their diploid parents，while the lignin content was significantly lower at some developmental stage. In conclusion，segmental membrane thickness，water-soluble pectin，protopectin，cellulose and hemicellulose contents are important factors affecting the mastication of some citrus triploid hybrids，providing some useful evaluation indexes to screen superior triploid lines with good fruit mastication quickly from a large-scale triploid population.

Key words: Citrus, triploid hybrids, segmental membrane, mastication, cellulose content

CAO Huixiang, LUO Xin, LIU Wanrong, GUAN Shuping, WANG Tingting, WU Xiaomeng, GUO Wenwu, XIE Kaidong. Evaluation of Fruit Segmental Membrane Development and Mastication in Two Citrus Triploids[J]. Acta Horticulturae Sinica, 2024, 51(6): 1179-1188.

Figures/Tables 5

Fig. 1 Capsule hardness and shear force determination of the segmental membranes in two triploid hybrids（BDZSD-3x，HJSD-3x）and their corresponding diploid parents（BDZ-2x，HJ-2x） t-test，* α = 0.05，** α = 0.01.

Fig. 2 Cytological observation of the segmental membranes in the two triploid hybrids（BDZSD-3x，HJSD-3x）and their corresponding diploid parents（BDZ-2x，HJ-2x）60-120 d after anthesis

Fig. 3 Thickness and cell layer number determination of the segmental membranes in the two triploid hybrids（BDZSD-3x，HJSD-3x）with their corresponding diploid parents（BDZ-2x，HJ-2x）120-210 d after anthesis t-test，* α = 0.05，** α = 0.01.

Fig. 4 Ultrastructural comparison of the segmental membranes in the two triploid hybrids（BDZSD-3x，HJSD-3x）and their corresponding diploid parents（BDZ-2x，HJ-2x）90，150 and 210 d after anthesis

Fig. 5 Cellulose，hemicellulose，lignin，water-soluble pectin and protopectin contents in the segmental membranes in the two triploid hybrids（BDZSD-3x，HJSD-3x）and their corresponding diploid parents（BDZ-2x，HJ-2x）90-210 after anthesis t-test，* α = 0.05，** α = 0.01.

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Evaluation of Fruit Segmental Membrane Development and Mastication in Two Citrus Triploids

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