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Acta Horticulturae Sinica ›› 2024, Vol. 51 ›› Issue (5): 956-970.doi: 10.16420/j.issn.0513-353x.2023-0273

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The Role of BAG1 in the Infection of Citrus with Alternaria Brown Spot

ZHOU Huizhen, ZHANG Jia, HU Junhua*(), LI Baixue, CAO Li, YU Xin, WANG Fusheng, ZOU Xiuping, ZHOU Yan   

  1. Citrus Research Institute,Southwest University,National Citrus Engineering Technology Research Center,Chongqing,400712,China
  • Received:2024-01-03 Revised:2024-05-09 Online:2024-05-25 Published:2024-05-29

Abstract:

In this paper,the regulatory mechanism of citrus BAG1 gene in response to Alternaria alternata infection was explored. BAG1 was amplified from the leaves of‘Chengtuo Hongju’(Citrus tangerina Hort.ex Tanaka),‘Miyagawa Wase Unshiu’(C. unshiu Macf),‘Ehime 38’(C. reticulata Blanco)and‘Jinqiu Shatangju’(C. reticulata Blanco). BAG1 protein was relatively conserved and had Ubiquitin domain and BAG domain. There are only three amino acid changes in the BAG domain of Ehime 38,resulting in changes in the side chain groups. The upstream promoter sequence showed that the four citrus cultivars contained the same hormone and stress-related cis-acting elements. Systematic analysis showed that the four citrus cultivars had the highest similarity to the BAG1 protein of mandarin,and had a distant relationship with sweet oranges and pomelo. qRT-PCR showed that the expression of BAG1 gene was significantly different in the resistant and susceptible cirtus cultivars during infected by A. alternata strain THJ. Hormonal stress treatment showed that BAG1 was up-regulated by exogenous plant hormones MeJA,ABA and SA. Trasient expression of tobacco showed that BAG1 protein was localized to the cell nucleus and cell membrane. BAG1 gene silencing and overexpression showed that BAG1 silencing could enhance the resistance of citrus to A.alternata. Overexpression reduced the resistance to A.alternata. The results showed that citrus BAG1 was involved in the regulation of A. alternata by participating in ROS excitation and ABA and JA signaling pathways.

Key words: Citrus, BAG1, Alternaria alternata, susceptibility gene, reactive oxygen species