Acta Horticulturae Sinica ›› 2024, Vol. 51 ›› Issue (5): 956-970.doi: 10.16420/j.issn.0513-353x.2023-0273
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ZHOU Huizhen, ZHANG Jia, HU Junhua*(), LI Baixue, CAO Li, YU Xin, WANG Fusheng, ZOU Xiuping, ZHOU Yan
Received:
2024-01-03
Revised:
2024-05-09
Online:
2024-05-25
Published:
2024-05-29
ZHOU Huizhen, ZHANG Jia, HU Junhua, LI Baixue, CAO Li, YU Xin, WANG Fusheng, ZOU Xiuping, ZHOU Yan. The Role of BAG1 in the Infection of Citrus with Alternaria Brown Spot[J]. Acta Horticulturae Sinica, 2024, 51(5): 956-970.
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URL: https://www.ahs.ac.cn/EN/10.16420/j.issn.0513-353x.2023-0273
名称 Name | 上游引物序列(5′-3′) Forward primer sequence | 下游引物序列(5′-3′) Reverse primer sequence |
---|---|---|
CiBAG1 | ATGCTTTCATTGGCAAGAAGC | TCAATCAAAAGTTTCCCATTTTG |
qCiBAG1 | TAACCGAGAAAGACGGTGCC | TCCGTAACCTGCTGAGCAAA |
TRV-BAG1 | GCTCTAGAGCGGAAATTAGGCCTGGAGGAATG | CGAGCTCGCCAAATCCGTAACCTGCTGAGC |
PLGN-BAG1 | CCCCGGGGATGCTTTCATTGGCAAGAAGC | CGGAATTCCGTCAATCAAAAGTTTCCCATTTTG |
PCX-BAG1 | GCGTCGACGCATGCTTTCATTGGCAAGAAGC | CCCCGGGGTCAATCAAAAGTTTCCCA TTTTG |
TRV1 | TTGGGTTGCTACTGATTCGACT | CTGTAAGGACCATCATACTTCGC |
TRV2 | ATTCACTGGGAGATGATACGCT | GAATCTAAGTCCACTCGTCCGT |
QiBAG1 | GCATGAGACAATTGCTTCATATGG | CCCTTAAAGCCTTCGAGTGG |
Actin | GCCTTCCGTCTTGGGTCT | AGGGCGGTGATTTCCTT |
NtPR1 | CCGCCTTCCCTCAACTCAAC | GCACAACCAAGACGTACTGAG |
NtPR2 | AGGTGTTTGCTATGGAATGC | TCTGTACCCACCATCTTGC |
NtPR4 | GGCCAAGATTCCTGTGGTAGAT | CACTGTTGTTTGAGTTCCTGTTCCT |
NtActin | ACCAGATTAATGAGCCCAAGAG | CCAACAGGGACAGTACCAATAC |
CiNPR1 | GGCCTTTCCCTATAGGCGTT | AAATCAACAGCCGGCCTACA |
CiICS | ATTGATTGGCTCCATGCCCA | TGAGCTTTGGCCATTACCGT |
CiPDF1.2 | CAGTGGCAGAAGCAAAACAA | CCGGGGAAGTCGTAGTGGC |
CiLOX | GCATCCTTTCTTGATCGGTTTC | GGCAGGCTCGCCATGA |
CiAOC | AGATCGTGGCAGTCCAGCTT | GCTAAAAGGGACAAGATCACCAA |
CiAOS | GCTATACACTCCTCGGGTTCGA | TGAAATGGAGGCGGTGATC |
CiJAR1 | GCACCATCAGGCATCAGACA | CAATGCGTTGAGTGTGCGTT |
CiPOD | GACAGCAGAACAGCCAACAGG | GTTCGGCACTGAGCCCTTC |
CiSOD | TGGAAGGGCTGTTGTAGTCC | ATGATGCCGCAAGCTACTCT |
CiCAT | GTCTCAATGTGA GGCCAAGC | TAGGTGTCGGGAGCTTCTCT |
Plant actin | CATCCCTCAGCACCTTCC | CCAACCTTAGCACTTCTCC |
Table 1 Information of primers in this study
名称 Name | 上游引物序列(5′-3′) Forward primer sequence | 下游引物序列(5′-3′) Reverse primer sequence |
---|---|---|
CiBAG1 | ATGCTTTCATTGGCAAGAAGC | TCAATCAAAAGTTTCCCATTTTG |
qCiBAG1 | TAACCGAGAAAGACGGTGCC | TCCGTAACCTGCTGAGCAAA |
TRV-BAG1 | GCTCTAGAGCGGAAATTAGGCCTGGAGGAATG | CGAGCTCGCCAAATCCGTAACCTGCTGAGC |
PLGN-BAG1 | CCCCGGGGATGCTTTCATTGGCAAGAAGC | CGGAATTCCGTCAATCAAAAGTTTCCCATTTTG |
PCX-BAG1 | GCGTCGACGCATGCTTTCATTGGCAAGAAGC | CCCCGGGGTCAATCAAAAGTTTCCCA TTTTG |
TRV1 | TTGGGTTGCTACTGATTCGACT | CTGTAAGGACCATCATACTTCGC |
TRV2 | ATTCACTGGGAGATGATACGCT | GAATCTAAGTCCACTCGTCCGT |
QiBAG1 | GCATGAGACAATTGCTTCATATGG | CCCTTAAAGCCTTCGAGTGG |
Actin | GCCTTCCGTCTTGGGTCT | AGGGCGGTGATTTCCTT |
NtPR1 | CCGCCTTCCCTCAACTCAAC | GCACAACCAAGACGTACTGAG |
NtPR2 | AGGTGTTTGCTATGGAATGC | TCTGTACCCACCATCTTGC |
NtPR4 | GGCCAAGATTCCTGTGGTAGAT | CACTGTTGTTTGAGTTCCTGTTCCT |
NtActin | ACCAGATTAATGAGCCCAAGAG | CCAACAGGGACAGTACCAATAC |
CiNPR1 | GGCCTTTCCCTATAGGCGTT | AAATCAACAGCCGGCCTACA |
CiICS | ATTGATTGGCTCCATGCCCA | TGAGCTTTGGCCATTACCGT |
CiPDF1.2 | CAGTGGCAGAAGCAAAACAA | CCGGGGAAGTCGTAGTGGC |
CiLOX | GCATCCTTTCTTGATCGGTTTC | GGCAGGCTCGCCATGA |
CiAOC | AGATCGTGGCAGTCCAGCTT | GCTAAAAGGGACAAGATCACCAA |
CiAOS | GCTATACACTCCTCGGGTTCGA | TGAAATGGAGGCGGTGATC |
CiJAR1 | GCACCATCAGGCATCAGACA | CAATGCGTTGAGTGTGCGTT |
CiPOD | GACAGCAGAACAGCCAACAGG | GTTCGGCACTGAGCCCTTC |
CiSOD | TGGAAGGGCTGTTGTAGTCC | ATGATGCCGCAAGCTACTCT |
CiCAT | GTCTCAATGTGA GGCCAAGC | TAGGTGTCGGGAGCTTCTCT |
Plant actin | CATCCCTCAGCACCTTCC | CCAACCTTAGCACTTCTCC |
Fig. 1 Comparison of multiple sequences of BAG1 in five citrus cultivars with Arabidopsis BAG1 Ci:Lementina;H:Hongju;a:Ehime 38;J:Jinqiu Shatangju;G:Miyagawa;At:Arabidopsis. On the triangle are key residues that are highly conserved in Hsp70/Hsc70 protein binding.
功能 Function | 顺式元件 cis-Element | 序列 Sequence | 数量 Number | ||
---|---|---|---|---|---|
参与脱落酸响应Involved in the abscisic acid responsiveness | ABRE | ACGTG | 2 | ||
赤霉素响应Gibberellin-responsive element | GARE-motif | TCTGTTG | 1 | ||
厌氧诱导元件Essential for the anaerobic induction | ARE | AAACCA | 2 | ||
厌氧特异性诱导Involved in anoxic specific inducibility | GC-motif | CCCCCG | 1 | ||
干旱诱导性MYB binding site involved in drought-inducibility | MBS | CAACTG | 1 | ||
参与低温响应Involved in low-temperature responsiveness | LTR | CCGAAA | 1 | ||
部分光响应Part of a light responsive element | Box 4 | ATTAAT | 2 | ||
AE-box | AGAAACTT | 3 | |||
TCT-motif | TCTTAC | 4 | |||
LS7 | CAGATTTATTTTTA | 1 | |||
I-box | TGATAATGT | 1 | |||
ACE | CTAACGTATT | 1 | |||
GT1-motif | GGTTAA | 1 | |||
G-Box | CACGTT | 2 |
Table 2 The cis-acting elements prediction of the upstream 2 000 bp promoter region of CiBAG1
功能 Function | 顺式元件 cis-Element | 序列 Sequence | 数量 Number | ||
---|---|---|---|---|---|
参与脱落酸响应Involved in the abscisic acid responsiveness | ABRE | ACGTG | 2 | ||
赤霉素响应Gibberellin-responsive element | GARE-motif | TCTGTTG | 1 | ||
厌氧诱导元件Essential for the anaerobic induction | ARE | AAACCA | 2 | ||
厌氧特异性诱导Involved in anoxic specific inducibility | GC-motif | CCCCCG | 1 | ||
干旱诱导性MYB binding site involved in drought-inducibility | MBS | CAACTG | 1 | ||
参与低温响应Involved in low-temperature responsiveness | LTR | CCGAAA | 1 | ||
部分光响应Part of a light responsive element | Box 4 | ATTAAT | 2 | ||
AE-box | AGAAACTT | 3 | |||
TCT-motif | TCTTAC | 4 | |||
LS7 | CAGATTTATTTTTA | 1 | |||
I-box | TGATAATGT | 1 | |||
ACE | CTAACGTATT | 1 | |||
GT1-motif | GGTTAA | 1 | |||
G-Box | CACGTT | 2 |
Fig. 3 The expression pattern of BAG1 in‘Hongju’and‘Miyagawa’at different time points after Alternaria alternata srain THJ infection * indicates significant difference(P < 0.05),*** indicates very significant difference(P < 0.001).
Fig. 4 The expression pattern of BAG1 gene in‘Hongju’under plant growth regulators stress * indicates significant difference(P < 0.05),** indicates extremely significant difference(P < 0.01),*** indicates very significant difference(P < 0.001).
Fig. 5 ‘Hongju’VIGS transient silence a:BAG1 gene expression of control and silenced plants;b:Symptoms of control and silenced plants(pTRV2-BAG1-1/2/3)inoculated with A. alternata strain THJ;c:Average disease spot diameter of control and silenced plants after A. alternata inoculation. * indicates significant difference(P < 0.05);** indicates extremely significant difference(P < 0.01).
Fig. 6 The expression level of BAG1(a)and the phenotype after inoculation with THJ strain(b),spot diameter(c)and related gene expression of‘Hongju’transient overexpression strain(d) WT:Wild-type;PLGN:Negative control;PLGN-BAG1:Overexpressed strain. ns indicates no significant difference;* indicates significant difference(P < 0.05);** indicates extremely significant difference(P < 0.01).
Fig. 7 Gene expression determination and staining observation of transient overexpression of BAG1 in tobacco leaves a:Expression analysis of BAG1 in Nicotiana benthamiana(tobacco)leaves;b:Quantification of A. alternata biomass in BAG1 transgenic tobacco leaves;c:Relative expression of pathogensis related protein genes in empty vector(PCX)and BAG1 overexpressed tobacco before and after inoculation of A. alternata;d:Staining observation. PCX,infiltrated with Agrobacterium containing empty vector(PCX). PCX-BAG1,infiltrated with Agrobacterium harboring recombinant plasmid(PCX-BAG1). (ⅰ):Leaf injection site;(ⅱ):Diaminobenzidine staining (DAB)of tobacco leaves three days after infiltration;(ⅲ):Typan blue staining(TPB)of tobacco leaves three days after infiltration. * P < 0.05;*** P < 0.01.
[1] |
doi: 10.3389/fpls.2019.00911 pmid: 31428107 |
[2] |
|
[3] |
|
[4] |
|
[5] |
|
[6] |
|
[7] |
doi: 10.1074/jbc.M511794200 pmid: 16636050 |
[8] |
doi: 10.1007/s00109-011-0795-6 pmid: 21818581 |
[9] |
|
[10] |
|
宫金礼. 2021. 红光调控柑橘果实转色的分子及细胞生物学机制研究[博士论文]. 武汉: 华中农业大学.
|
|
[11] |
|
[12] |
|
[13] |
pmid: 16003391 |
[14] |
|
[15] |
|
[16] |
|
[17] |
|
[18] |
|
刘庆霞, 李梦莎, 国静. 2012. 茉莉酸生物合成的调控及其信号通路. 植物生理学报, 48 (9):837-844.
|
|
[19] |
|
龙艳玲, 苏基平, 胡军华, 王帆, 王娟, 姚廷山, 龚桂芝, 周彦. 2018. 柑桔褐斑病菌侵染对不同抗性柑桔种质防御酶活性的影响. 中国南方果树, 47 (1):6-11,16.
|
|
[20] |
|
栾威, 李鑫雨, 代娅, 王艳, 李彩侠, 马欣荣. 2022. 外源ABA对番茄抗性相关基因表达的影响. 应用与环境生物学报, 28:582-587.
|
|
[21] |
doi: 10.1094/PHYTO-95-0241 pmid: 18943116 |
[22] |
|
[23] |
|
[24] |
doi: 10.1002/bit.21075 pmid: 16983697 |
[25] |
|
[26] |
|
[27] |
|
[28] |
|
王佳丽. 2019. 辅助分子伴侣SlBAG蛋白在番茄抗病反应中的功能研究[硕士论文]. 杭州: 浙江大学.
|
|
[29] |
|
魏婧, 徐畅, 李可欣, 贺洪军, 徐启江. 2020. 超氧化物歧化酶的研究进展与植物抗逆性. 植物生理学报, 56 (12):2571-2584.
|
|
[30] |
|
[31] |
|
张庆雨. 2014. 草莓BAG6基因在植物炭疽病抗性中的作用研究[硕士论文]. 杨凌: 西北农林科技大学.
|
|
[32] |
|
赵晓莉, 卢晓鹏, 聂琼, 黄成能, 肖玉明, 谢深喜. 2013. 水分胁迫对柑橘生理指标及JA合成相关酶基因表达的影响. 江西农业大学学报, 35 (3):530-535.
|
|
[33] |
|
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