Acta Horticulturae Sinica ›› 2022, Vol. 49 ›› Issue (3): 613-621.doi: 10.16420/j.issn.0513-353x.2021-0018

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Prediction of Fruit Color Genes Under the Calyx of Eggplant Based on Genome-wide Resequencing in an Extreme Mixing Pool

QIAO Jun, LIU Jing, LI Suwen, WANG Liying*()   

  1. State Key Laboratory of Vegetable Germplasm Innovation,Tianjin Enterprise Key Laboratory of Vegetable Genetics & Breeding,Tianjin Kernel Vegetable Research Institute,Tianjin 300381,China
  • Received:2021-08-02 Revised:2021-11-25 Online:2022-03-25 Published:2022-03-25
  • Contact: WANG Liying


Stably genetic purple under the calyx,round eggplant inbred line Y5 was crossed with green under the calyx,round eggplant inbred line Y73. The segregation of fruit color under the calyx of F2 progeny had a normal distribution,which could be analyzed by bulk segregant analysis(BSA). In the F2 population,30 purple and 30 green under the calyx plants were selected to construct separated mixed pools. Whole-genome resequencing of 30× and 10× coverage was carried out in the mixed pools and parents to locate the correlation interval of fruit color under the calyx. Candidate genes were predicted according to the collinear region and gene annotation information of the eggplant genome. A total of 7 747 547 single nucleotide polymorphisms(SNP)and 569 796 insertion deletion markers(InDel)were obtained from the hybrid pools and parents,which were used for genome-wide mapping of fruit color traits under the calyx. According to pathway enrichment and gene function annotation,candidate genes EGP21397,EGP21400,EGP21491,EGP22288,and EGP22289 were obtained. The five candidate genes were closely related to cell photosynthesis and encoded calmodulin-binding family proteins,the C2H2 zinc finger protein,carotenoid isomerase,2Fe-2S ferredoxin-like superfamily proteins,and the lipid transfer protein. At the same time,six candidate genes related to cytochrome P450 and phytochrome synthesis were predicted,which were located in the association regions of chromosomes 10. These proteins and pigments were related to photosynthesis and stress development,which revealed the molecular mechanism of fruit color in eggplant.

Key words: eggplant, fruit color under the calyx, gene mapping, extreme mixing pool, whole-genome resequencing

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