Antifungal Activity of Pseudomonas protegens FD6 Against Botrytis cinerea and Optimization of Its Culture Conditions

doi:10.16420/j.issn.0513-353x.2024-0829

Acta Horticulturae Sinica ›› 2025, Vol. 52 ›› Issue (9): 2545-2553.doi: 10.16420/j.issn.0513-353x.2024-0829

• Plant Protection • Previous Articles Next Articles

Antifungal Activity of Pseudomonas protegens FD6 Against Botrytis cinerea and Optimization of Its Culture Conditions

SONG Qi¹, SONG Xiaoya¹, JIAO Yongxin¹, MAO Ziyue¹, WU Xiaogang², ZHANG Qingxia¹^,^*()

¹ College of Plant Protection， Yangzhou University，Yangzhou， Jiangsu 225009， China
² Guangxi Key Laboratory of Agro-environment and Agro-product Safety， College of Agriculture，Guangxi University， Nanning 530004， China

Received:2024-11-27 Revised:2025-05-30 Online:2025-09-25 Published:2025-09-24
Contact: ZHANG Qingxia

Abstract

Abstract:

Pseudomonas protegens FD6 can effectively control tomato gray mold by producing antibiotics pyoluteorin and 2,4-diacetylphloroglucinol. The yield of bacterial secondary metabolites depends not only on the strain，but also the fermentation conditions. In order to improve the antifungal activity of FD6，the antagonistic ability of culture filtrates from six different media and LB filtrate displayed the best antifungal activity were assayed. Then LB were used as the basic fermentation medium to optimize the medium composition and fermentation conditions of FD6 through single-factor test and response surface method. The results showed that the optimal fermentation conditions of strain FD6 were as follows：sodium citrate 10.81 g · L^-1，peptone 10.53 g · L^-1，valine 5.28 g · L^-1，pH 7.2，28 ℃，cultivation time 120 h. After optimization，the average inhibition rate of the sterile filtrate of FD6 against Botrytis cinerea was 80.26%，representing a 39.39% increase compared to pre-optimization levels. The quantitative analysis of antibiotics revealed that the production of PLT reached 14.85 μg · mL^-1 after optimization，and caused a 4.71-fold increase compared with pre-optimization levels. Our results provide a valuable reference for the development of P. protegens FD6 bacterial agent and its application in biological control of tomato gray mold.

Key words: tomato, Pseudomonas protegens, culture condition, optimization, single factor experiment, response surface, Botrytis cinerea

SONG Qi, SONG Xiaoya, JIAO Yongxin, MAO Ziyue, WU Xiaogang, ZHANG Qingxia. Antifungal Activity of Pseudomonas protegens FD6 Against Botrytis cinerea and Optimization of Its Culture Conditions[J]. Acta Horticulturae Sinica, 2025, 52(9): 2545-2553.

Figures/Tables 5

Fig. 1 The inhibitory effect of the fermentation supernatant of Pseudomonas protegens FD6 on Botrytis cinerea in six media Different lowercase letters indicate significant differences（P < 0.05），the same below

Fig. 2 Effects of different carbon source（A），nitrogen sources（B）and amino acid（C）on the inhibition of Botrytis cinerea by Pseudomonas protegens FD6

Fig. 3 Effects of different inorganic ions on the inhibition of Botrytis cinerea by Pseudomonas protegens FD6

Fig. 4 Effects of different culture conditions on the inhibition of Botrytis cinerea by Pseudomonas protegens FD6

Fig. 5 Response surface diagram of the interaction effect of sodium citrate and peptone，sodium citrate and valine，peptone and valine on the inhibition rate

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Antifungal Activity of Pseudomonas protegens FD6 Against Botrytis cinerea and Optimization of Its Culture Conditions

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